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Isolation and characterization of chromobacterium sp. from lake water at Manipal International University

Introduction: The violet-pigmented Chromobacterium spp. are vastly located in soil and surface water of subtropical regions. Majority of the species have been identified as highly potential in bio-industries; however, the bacterial pathogenicity is largely understudied. These bacteria are resistant...

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Main Authors: Sandrasaigaran, Pratheep, Rajandrai, Pavitraa, Mak, Woei Loon, Hasan, Hanan
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2021
Online Access:http://psasir.upm.edu.my/id/eprint/90355/1/2021040613124821_MJMHS_0643.pdf
http://psasir.upm.edu.my/id/eprint/90355/
https://medic.upm.edu.my/upload/dokumen/2021040613124821_MJMHS_0643.pdf
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spelling my.upm.eprints.903552021-09-10T00:58:07Z http://psasir.upm.edu.my/id/eprint/90355/ Isolation and characterization of chromobacterium sp. from lake water at Manipal International University Sandrasaigaran, Pratheep Rajandrai, Pavitraa Mak, Woei Loon Hasan, Hanan Introduction: The violet-pigmented Chromobacterium spp. are vastly located in soil and surface water of subtropical regions. Majority of the species have been identified as highly potential in bio-industries; however, the bacterial pathogenicity is largely understudied. These bacteria are resistant to multiple-drugs and infections may cause sepsis and liver abscissions. Thus, this study aimed to characterize the violet-pigmented bacteria isolated from the lake in Manipal International University and further examine its antibiotic susceptibility. Methods: The isolated violet bacteria (Dyh27s2016) were subjected to the morphology, physiology, biochemical and antibiotic susceptibility tests. Also, the species were scrutinized via the 16S rRNA and phylogenetic analysis. In addition, the lake water physicochemical properties were examined to understand the bacterial adaptability in this region. Results: Dyh27s2016 strain was found to exhibit similar morphology and physiology characteristics to Chromobacterium spp. and be closely related to Chromobacterium amazonense (98% sequence-homology). However, the biochemical analysis indicated that this strain was capable of indole production; contrarily, Chromobacterium spp. were found mostly indole negative. On top of that, this strain also tested resistant to most β-lactam and aminoglycoside antibiotics. The adaptability of the Dyh27s2016 strain in this region might be supported by the satisfactory physiochemical properties of the lake water and mainly by the low dissolve oxygen concentration. Conclusion: The morphology, physiology, biochemical and molecular characterizations of Dyh27s2016 isolate show high similarity to Chromobacterium spp. and the multi-drug resistance of this strain can potentially harbour a threat to public health if contacted by humans or animals via food or water. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2021-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/90355/1/2021040613124821_MJMHS_0643.pdf Sandrasaigaran, Pratheep and Rajandrai, Pavitraa and Mak, Woei Loon and Hasan, Hanan (2021) Isolation and characterization of chromobacterium sp. from lake water at Manipal International University. Malaysian Journal of Medicine and Health Sciences, 17 (2). pp. 154-161. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2021040613124821_MJMHS_0643.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: The violet-pigmented Chromobacterium spp. are vastly located in soil and surface water of subtropical regions. Majority of the species have been identified as highly potential in bio-industries; however, the bacterial pathogenicity is largely understudied. These bacteria are resistant to multiple-drugs and infections may cause sepsis and liver abscissions. Thus, this study aimed to characterize the violet-pigmented bacteria isolated from the lake in Manipal International University and further examine its antibiotic susceptibility. Methods: The isolated violet bacteria (Dyh27s2016) were subjected to the morphology, physiology, biochemical and antibiotic susceptibility tests. Also, the species were scrutinized via the 16S rRNA and phylogenetic analysis. In addition, the lake water physicochemical properties were examined to understand the bacterial adaptability in this region. Results: Dyh27s2016 strain was found to exhibit similar morphology and physiology characteristics to Chromobacterium spp. and be closely related to Chromobacterium amazonense (98% sequence-homology). However, the biochemical analysis indicated that this strain was capable of indole production; contrarily, Chromobacterium spp. were found mostly indole negative. On top of that, this strain also tested resistant to most β-lactam and aminoglycoside antibiotics. The adaptability of the Dyh27s2016 strain in this region might be supported by the satisfactory physiochemical properties of the lake water and mainly by the low dissolve oxygen concentration. Conclusion: The morphology, physiology, biochemical and molecular characterizations of Dyh27s2016 isolate show high similarity to Chromobacterium spp. and the multi-drug resistance of this strain can potentially harbour a threat to public health if contacted by humans or animals via food or water.
format Article
author Sandrasaigaran, Pratheep
Rajandrai, Pavitraa
Mak, Woei Loon
Hasan, Hanan
spellingShingle Sandrasaigaran, Pratheep
Rajandrai, Pavitraa
Mak, Woei Loon
Hasan, Hanan
Isolation and characterization of chromobacterium sp. from lake water at Manipal International University
author_facet Sandrasaigaran, Pratheep
Rajandrai, Pavitraa
Mak, Woei Loon
Hasan, Hanan
author_sort Sandrasaigaran, Pratheep
title Isolation and characterization of chromobacterium sp. from lake water at Manipal International University
title_short Isolation and characterization of chromobacterium sp. from lake water at Manipal International University
title_full Isolation and characterization of chromobacterium sp. from lake water at Manipal International University
title_fullStr Isolation and characterization of chromobacterium sp. from lake water at Manipal International University
title_full_unstemmed Isolation and characterization of chromobacterium sp. from lake water at Manipal International University
title_sort isolation and characterization of chromobacterium sp. from lake water at manipal international university
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/90355/1/2021040613124821_MJMHS_0643.pdf
http://psasir.upm.edu.my/id/eprint/90355/
https://medic.upm.edu.my/upload/dokumen/2021040613124821_MJMHS_0643.pdf
_version_ 1712286775812030464
score 13.211869

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