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In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound

Background: Malaria is a serious disease that is causing huge toll on human health. The parasites causing this disease have developed resistance toward the current mainstream drugs. Tinospora crispa Miers is a well-known ethnopharmacological plant of Malaysia and has been traditionally used to treat...

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Main Authors: Lee, Wei Cai, Mahmud, Roziahanim, Perumal, Shanmugapriya, Ismail, Sabariah, Basir, Rusliza
Format: Article
Language:English
Published: Medknow Publications 2020
Online Access:http://psasir.upm.edu.my/id/eprint/89336/1/MIER.pdf
http://psasir.upm.edu.my/id/eprint/89336/
https://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=67;spage=76;epage=82;aulast=Lee
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spelling my.upm.eprints.893362021-09-20T22:31:51Z http://psasir.upm.edu.my/id/eprint/89336/ In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound Lee, Wei Cai Mahmud, Roziahanim Perumal, Shanmugapriya Ismail, Sabariah Basir, Rusliza Background: Malaria is a serious disease that is causing huge toll on human health. The parasites causing this disease have developed resistance toward the current mainstream drugs. Tinospora crispa Miers is a well-known ethnopharmacological plant of Malaysia and has been traditionally used to treat malaria, fever, and other parasite-related illnesses. Objective: The present study investigated the potential of the crude methanol extract and antiparasitic fractions of T. crispa stem to exert antimalarial activity against Plasmodium berghei. Materials and Methods: The potent antiparasitic fractions of T. crispa, F4, and F5 were isolated in the previous study against Toxoplasma gondii. The same antiparasitic fractions along with crude methanol extract of different doses (10, 50, 100 mg/kg b. w.) were employed in the present study to determine the antimalarial activity against P. berghei ANKA strain. The survival curves of the treated mice were plotted by employing the log-rank (Mantel–Cox) test. The chemical composition of the most potent fraction F5 was determined spectrometrically using electrospray ionization-mass spectrometry (MS). Results: In a murine P. berghei model, fraction F5 displayed the highest parasitemia suppression effects compared to the crude methanol extract and other fraction. Subsequent chemical analysis by MS on fraction F5 has led to the tentative identification of 13-hydroperoxyoctadeca-9, 11-dienoic acid (13[S]-HPODE) compound. Conclusion: The crude methanol extract of T. crispa and its fraction F5 possess potent antimalarial activities, and the tentative discovery of the 13(S)-HPODE bioactive compound may serve as a precursor for developing a semisynthetic antiparasitic drug with enhanced efficacy and low toxicity. Medknow Publications 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89336/1/MIER.pdf Lee, Wei Cai and Mahmud, Roziahanim and Perumal, Shanmugapriya and Ismail, Sabariah and Basir, Rusliza (2020) In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound. Pharmacognosy Magazine, 16 (67). 76 - 82. ISSN 0973-1296; ESSN: 0976-4062 https://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=67;spage=76;epage=82;aulast=Lee 10.4103/pm.pm_10_19
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Malaria is a serious disease that is causing huge toll on human health. The parasites causing this disease have developed resistance toward the current mainstream drugs. Tinospora crispa Miers is a well-known ethnopharmacological plant of Malaysia and has been traditionally used to treat malaria, fever, and other parasite-related illnesses. Objective: The present study investigated the potential of the crude methanol extract and antiparasitic fractions of T. crispa stem to exert antimalarial activity against Plasmodium berghei. Materials and Methods: The potent antiparasitic fractions of T. crispa, F4, and F5 were isolated in the previous study against Toxoplasma gondii. The same antiparasitic fractions along with crude methanol extract of different doses (10, 50, 100 mg/kg b. w.) were employed in the present study to determine the antimalarial activity against P. berghei ANKA strain. The survival curves of the treated mice were plotted by employing the log-rank (Mantel–Cox) test. The chemical composition of the most potent fraction F5 was determined spectrometrically using electrospray ionization-mass spectrometry (MS). Results: In a murine P. berghei model, fraction F5 displayed the highest parasitemia suppression effects compared to the crude methanol extract and other fraction. Subsequent chemical analysis by MS on fraction F5 has led to the tentative identification of 13-hydroperoxyoctadeca-9, 11-dienoic acid (13[S]-HPODE) compound. Conclusion: The crude methanol extract of T. crispa and its fraction F5 possess potent antimalarial activities, and the tentative discovery of the 13(S)-HPODE bioactive compound may serve as a precursor for developing a semisynthetic antiparasitic drug with enhanced efficacy and low toxicity.
format Article
author Lee, Wei Cai
Mahmud, Roziahanim
Perumal, Shanmugapriya
Ismail, Sabariah
Basir, Rusliza
spellingShingle Lee, Wei Cai
Mahmud, Roziahanim
Perumal, Shanmugapriya
Ismail, Sabariah
Basir, Rusliza
In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound
author_facet Lee, Wei Cai
Mahmud, Roziahanim
Perumal, Shanmugapriya
Ismail, Sabariah
Basir, Rusliza
author_sort Lee, Wei Cai
title In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound
title_short In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound
title_full In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound
title_fullStr In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound
title_full_unstemmed In vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound
title_sort in vivo antimalarial potential of tinospora crispa miers in mice and identification of the bioactive compound
publisher Medknow Publications
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/89336/1/MIER.pdf
http://psasir.upm.edu.my/id/eprint/89336/
https://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=67;spage=76;epage=82;aulast=Lee
_version_ 1712286761910009856
score 13.211869

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