Staff View: In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)

In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)

Clinacanthus nutans, commonly known as Sabah snake grass, is one of the more important medicinal plants in Malaysia’s herbal industry. C. nutans has gained the attention of medical practitioners due to its wide range of bioactive compounds responsible for various biological activities, such as anti-...

Full description

Saved in:

Bibliographic Details
Main Authors:	Zainol, Haida, Jaafar, Juju Nakasha, Awang @ Mansor, Mohd Hakiman
Format:	Article
Language:	English
Published:	Multidisciplinary Digital Publishing Institute 2020
Online Access:	http://psasir.upm.edu.my/id/eprint/89334/1/NUTAN.pdf http://psasir.upm.edu.my/id/eprint/89334/ https://www.mdpi.com/2223-7747/9/8/1030
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.upm.eprints.89334
record_format	eprints
spelling	my.upm.eprints.893342021-09-03T05:38:09Z http://psasir.upm.edu.my/id/eprint/89334/ In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass) Zainol, Haida Jaafar, Juju Nakasha Awang @ Mansor, Mohd Hakiman Clinacanthus nutans, commonly known as Sabah snake grass, is one of the more important medicinal plants in Malaysia’s herbal industry. C. nutans has gained the attention of medical practitioners due to its wide range of bioactive compounds responsible for various biological activities, such as anti-cancer, anti-venom and anti-viral activities. Due to its high pharmacological properties, the species has been overexploited to meet the demands of the pharmaceutical industry. The present study was conducted to establish a suitable in vitro culture procedure for the mass propagation of C. nutans. Murashige and Skoog (MS) basal medium, supplemented with different types of cytokinins, auxins, basal medium strength and sucrose concentrations, were tested. Based on the results, a full-strength MS basal medium supplemented with 12 µM 6-benzylaminopurine (BAP) and 30 g/L sucrose was recorded as the best outcome for all the parameters measured including the regeneration percentage, number of shoots, length of shoots, number of leaves and fresh weight of leaves. In the analysis of the phenolics content and antioxidant activities, tissue-cultured leaf extracts assayed at 100 °C exhibited the highest phenolic content and antioxidant activities. The propagation of C. nutans via a plant tissue culture technique was recorded to be able to produce high phenolic contents as well as exhibit high antioxidant activities. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89334/1/NUTAN.pdf Zainol, Haida and Jaafar, Juju Nakasha and Awang @ Mansor, Mohd Hakiman (2020) In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass). Plants-Basel, 9 (8). art. no. 1030. pp. 1-17. ISSN 2223-7747 https://www.mdpi.com/2223-7747/9/8/1030 10.3390/plants9081030
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Clinacanthus nutans, commonly known as Sabah snake grass, is one of the more important medicinal plants in Malaysia’s herbal industry. C. nutans has gained the attention of medical practitioners due to its wide range of bioactive compounds responsible for various biological activities, such as anti-cancer, anti-venom and anti-viral activities. Due to its high pharmacological properties, the species has been overexploited to meet the demands of the pharmaceutical industry. The present study was conducted to establish a suitable in vitro culture procedure for the mass propagation of C. nutans. Murashige and Skoog (MS) basal medium, supplemented with different types of cytokinins, auxins, basal medium strength and sucrose concentrations, were tested. Based on the results, a full-strength MS basal medium supplemented with 12 µM 6-benzylaminopurine (BAP) and 30 g/L sucrose was recorded as the best outcome for all the parameters measured including the regeneration percentage, number of shoots, length of shoots, number of leaves and fresh weight of leaves. In the analysis of the phenolics content and antioxidant activities, tissue-cultured leaf extracts assayed at 100 °C exhibited the highest phenolic content and antioxidant activities. The propagation of C. nutans via a plant tissue culture technique was recorded to be able to produce high phenolic contents as well as exhibit high antioxidant activities.
format	Article
author	Zainol, Haida Jaafar, Juju Nakasha Awang @ Mansor, Mohd Hakiman
spellingShingle	Zainol, Haida Jaafar, Juju Nakasha Awang @ Mansor, Mohd Hakiman In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)
author_facet	Zainol, Haida Jaafar, Juju Nakasha Awang @ Mansor, Mohd Hakiman
author_sort	Zainol, Haida
title	In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)
title_short	In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)
title_full	In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)
title_fullStr	In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)
title_full_unstemmed	In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)
title_sort	in vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of clinacanthus nutans (sabah snake grass)
publisher	Multidisciplinary Digital Publishing Institute
publishDate	2020
url	http://psasir.upm.edu.my/id/eprint/89334/1/NUTAN.pdf http://psasir.upm.edu.my/id/eprint/89334/ https://www.mdpi.com/2223-7747/9/8/1030
_version_	1710677180922462208
score	13.211869

In vitro responses of plant growth factors on growth, yield, phenolics content and antioxidant activities of Clinacanthus nutans (Sabah snake grass)

Similar Items