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Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides

Free biocatalyst such as lipase cannot be recovered and reused; and is extremely unstable in harsh reaction condition. Present study aimed to synthesize hierarchical ordered macro-microporous ZIF-8 structures (MAC-ZIF-8) for encapsulation of nano-Burkholderia cepacia lipase (nano BCL). The synthesiz...

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Main Authors: Cheng, Yongfa, Ma, Baokai, Tan, Chin Ping, Lai, Oi Ming, Panpipat, Worawan, Cheong, Ling Zhi, Shen, Cai
Format: Article
Language:English
Published: Elsevier 2020
Online Access:http://psasir.upm.edu.my/id/eprint/89177/1/LIPASE.pdf
http://psasir.upm.edu.my/id/eprint/89177/
https://www.sciencedirect.com/science/article/abs/pii/S0925400520308224
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spelling my.upm.eprints.891772021-09-03T23:28:24Z http://psasir.upm.edu.my/id/eprint/89177/ Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides Cheng, Yongfa Ma, Baokai Tan, Chin Ping Lai, Oi Ming Panpipat, Worawan Cheong, Ling Zhi Shen, Cai Free biocatalyst such as lipase cannot be recovered and reused; and is extremely unstable in harsh reaction condition. Present study aimed to synthesize hierarchical ordered macro-microporous ZIF-8 structures (MAC-ZIF-8) for encapsulation of nano-Burkholderia cepacia lipase (nano BCL). The synthesized MAC-ZIF-8 has good crystallinity, thermal stability and high surface area which made it suitable for immobilization of nano-BCL. The MAC-ZIF-8 immobilized nano-BCL (nano-BCL@MAC-ZIF-8) demonstrated high enzyme activity (4.3778 U) and can be recovered and reused for consecutive hydrolysis reaction. It is found that the nano-BCL is well encapsulated with the macropores of the MAC-ZIF-8 structures and they are able to preserve their native configuration with high affinity for substrates (lower Km of 1.068 mM as compared to that of nano-BCL@ZIF-8). The nano-BCL@MAC-ZIF-8 can also be used to fabricate electrochemical biosensors for detection of nitrogenous diphenyl ether pesticides (nitrofen) in real food systems (apricots). The fabricated biosensors demonstrated wide linear range (0−114µM), low limit of detection (0.46µM) and good recovery rate. In comparison to conventional detection method, the fabricated biosensor also has added advantages in the form of easy and convenient operation, low cost, and better linear range. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89177/1/LIPASE.pdf Cheng, Yongfa and Ma, Baokai and Tan, Chin Ping and Lai, Oi Ming and Panpipat, Worawan and Cheong, Ling Zhi and Shen, Cai (2020) Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides. Sensors and Actuators B: Chemical, 321. art. no. 128477. pp. 1-9. ISSN 0925-4005 https://www.sciencedirect.com/science/article/abs/pii/S0925400520308224 10.1016/j.snb.2020.128477
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Free biocatalyst such as lipase cannot be recovered and reused; and is extremely unstable in harsh reaction condition. Present study aimed to synthesize hierarchical ordered macro-microporous ZIF-8 structures (MAC-ZIF-8) for encapsulation of nano-Burkholderia cepacia lipase (nano BCL). The synthesized MAC-ZIF-8 has good crystallinity, thermal stability and high surface area which made it suitable for immobilization of nano-BCL. The MAC-ZIF-8 immobilized nano-BCL (nano-BCL@MAC-ZIF-8) demonstrated high enzyme activity (4.3778 U) and can be recovered and reused for consecutive hydrolysis reaction. It is found that the nano-BCL is well encapsulated with the macropores of the MAC-ZIF-8 structures and they are able to preserve their native configuration with high affinity for substrates (lower Km of 1.068 mM as compared to that of nano-BCL@ZIF-8). The nano-BCL@MAC-ZIF-8 can also be used to fabricate electrochemical biosensors for detection of nitrogenous diphenyl ether pesticides (nitrofen) in real food systems (apricots). The fabricated biosensors demonstrated wide linear range (0−114µM), low limit of detection (0.46µM) and good recovery rate. In comparison to conventional detection method, the fabricated biosensor also has added advantages in the form of easy and convenient operation, low cost, and better linear range.
format Article
author Cheng, Yongfa
Ma, Baokai
Tan, Chin Ping
Lai, Oi Ming
Panpipat, Worawan
Cheong, Ling Zhi
Shen, Cai
spellingShingle Cheng, Yongfa
Ma, Baokai
Tan, Chin Ping
Lai, Oi Ming
Panpipat, Worawan
Cheong, Ling Zhi
Shen, Cai
Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides
author_facet Cheng, Yongfa
Ma, Baokai
Tan, Chin Ping
Lai, Oi Ming
Panpipat, Worawan
Cheong, Ling Zhi
Shen, Cai
author_sort Cheng, Yongfa
title Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides
title_short Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides
title_full Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides
title_fullStr Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides
title_full_unstemmed Hierarchical macro-microporous ZIF-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides
title_sort hierarchical macro-microporous zif-8 nanostructures as efficient nano-lipase carriers for rapid and direct electrochemical detection of nitrogenous diphenyl ether pesticides
publisher Elsevier
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/89177/1/LIPASE.pdf
http://psasir.upm.edu.my/id/eprint/89177/
https://www.sciencedirect.com/science/article/abs/pii/S0925400520308224
_version_ 1710677170215452672
score 13.211869

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