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Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model

Vibrio harveyi causes vibriosis in various commercial marine fish species. The infection leads to significant economic losses for aquaculture farms, and vaccination is an alternative approach for the prevention and control of fish diseases for aquaculture sustainability. This study describes the use...

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Main Authors: Abu Nor, Nadirah, Saad, Mohd Zamri, Md Yasin, Ina Salwany, Salleh, Annas, Mustaffa Kamal, Farina, Matori, Mohd Fuad, Azmai, Mohammad Noor Amal
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88748/1/RED.pdf
http://psasir.upm.edu.my/id/eprint/88748/
https://www.mdpi.com/2076-393X/8/4/734
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spelling my.upm.eprints.887482021-10-06T08:02:16Z http://psasir.upm.edu.my/id/eprint/88748/ Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model Abu Nor, Nadirah Saad, Mohd Zamri Md Yasin, Ina Salwany Salleh, Annas Mustaffa Kamal, Farina Matori, Mohd Fuad Azmai, Mohammad Noor Amal Vibrio harveyi causes vibriosis in various commercial marine fish species. The infection leads to significant economic losses for aquaculture farms, and vaccination is an alternative approach for the prevention and control of fish diseases for aquaculture sustainability. This study describes the use of formalin-killed Vibrio harveyi (FKVh) strain Vh1 as a vaccine candidate to stimulate innate and adaptive immunities against vibriosis in a marine red hybrid tilapia model. Tilapia are fast growing; cheap; resistant to diseases; and tolerant to adverse environmental conditions of fresh water, brackish water, and marine water and because of these advantages, marine red hybrid tilapia is a suitable candidate as a model to study fish diseases and vaccinations against vibriosis. A total of 180 healthy red hybrid tilapias were gradually adapted to the marine environment before being divided into two groups, with 90 fish in each group and were kept in triplicate with 30 fish per tank. Group 1 was vaccinated intraperitoneally with 100 µL of FKVh on week 0, and a booster dose was similarly administered on week 2. Group 2 was similarly injected with PBS. Skin mucus, serum, and gut lavage were collected weekly for enzyme-linked immunosorbent assay (ELISA) and a lysozyme activity assay from a total of 30 fish of each group. On week 4, the remaining 60 fish of Groups 1 and 2 were challenged with 108 cfu/fish of live Vibrio harveyi. The clinical signs were monitored while the survival rate was recorded for 48 h post-challenge. Vaccination with FKVh resulted in a significantly (p < 0.05) higher rate of survival (87%) compared to the control (20%). The IgM antibody titer and lysozyme activities of Group 1 were significantly (p < 0.05) higher than the unvaccinated Groups 2 in most weeks throughout the experiment. Therefore, the intraperitoneal exposure of marine red hybrid tilapia to killed V. harveyi enhanced the resistance and antibody response of the fish against vibriosis. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88748/1/RED.pdf Abu Nor, Nadirah and Saad, Mohd Zamri and Md Yasin, Ina Salwany and Salleh, Annas and Mustaffa Kamal, Farina and Matori, Mohd Fuad and Azmai, Mohammad Noor Amal (2020) Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model. Vaccines, 8 (4). pp. 1-14. ISSN 2076-393X https://www.mdpi.com/2076-393X/8/4/734 10.3390/vaccines8040734
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Vibrio harveyi causes vibriosis in various commercial marine fish species. The infection leads to significant economic losses for aquaculture farms, and vaccination is an alternative approach for the prevention and control of fish diseases for aquaculture sustainability. This study describes the use of formalin-killed Vibrio harveyi (FKVh) strain Vh1 as a vaccine candidate to stimulate innate and adaptive immunities against vibriosis in a marine red hybrid tilapia model. Tilapia are fast growing; cheap; resistant to diseases; and tolerant to adverse environmental conditions of fresh water, brackish water, and marine water and because of these advantages, marine red hybrid tilapia is a suitable candidate as a model to study fish diseases and vaccinations against vibriosis. A total of 180 healthy red hybrid tilapias were gradually adapted to the marine environment before being divided into two groups, with 90 fish in each group and were kept in triplicate with 30 fish per tank. Group 1 was vaccinated intraperitoneally with 100 µL of FKVh on week 0, and a booster dose was similarly administered on week 2. Group 2 was similarly injected with PBS. Skin mucus, serum, and gut lavage were collected weekly for enzyme-linked immunosorbent assay (ELISA) and a lysozyme activity assay from a total of 30 fish of each group. On week 4, the remaining 60 fish of Groups 1 and 2 were challenged with 108 cfu/fish of live Vibrio harveyi. The clinical signs were monitored while the survival rate was recorded for 48 h post-challenge. Vaccination with FKVh resulted in a significantly (p < 0.05) higher rate of survival (87%) compared to the control (20%). The IgM antibody titer and lysozyme activities of Group 1 were significantly (p < 0.05) higher than the unvaccinated Groups 2 in most weeks throughout the experiment. Therefore, the intraperitoneal exposure of marine red hybrid tilapia to killed V. harveyi enhanced the resistance and antibody response of the fish against vibriosis.
format Article
author Abu Nor, Nadirah
Saad, Mohd Zamri
Md Yasin, Ina Salwany
Salleh, Annas
Mustaffa Kamal, Farina
Matori, Mohd Fuad
Azmai, Mohammad Noor Amal
spellingShingle Abu Nor, Nadirah
Saad, Mohd Zamri
Md Yasin, Ina Salwany
Salleh, Annas
Mustaffa Kamal, Farina
Matori, Mohd Fuad
Azmai, Mohammad Noor Amal
Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model
author_facet Abu Nor, Nadirah
Saad, Mohd Zamri
Md Yasin, Ina Salwany
Salleh, Annas
Mustaffa Kamal, Farina
Matori, Mohd Fuad
Azmai, Mohammad Noor Amal
author_sort Abu Nor, Nadirah
title Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model
title_short Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model
title_full Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model
title_fullStr Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model
title_full_unstemmed Efficacy of whole cell inactivated Vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (Oreochromic niloticus x Oreo mossambicus) model
title_sort efficacy of whole cell inactivated vibrio harveyi vaccine against vibriosis in a marine red hybrid tilapia (oreochromic niloticus x oreo mossambicus) model
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/88748/1/RED.pdf
http://psasir.upm.edu.my/id/eprint/88748/
https://www.mdpi.com/2076-393X/8/4/734
_version_ 1713201301783838720
score 13.211869

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