Effective refolding of a cysteine rich glycoside hydrolase family 19 recombinant chitinase from Streptomyces griseus by reverse dilution and affinity chromatography
Conventional refolding methods are associated with low yields due to misfolding and high aggregation rates or very dilute proteins. In this study, we describe the optimization of the conventional methods of reverse dilution and affinity chromatography for obtaining high yields of a cysteine rich rec...
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| Main Authors: | , , , , , |
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| 格式: | Article |
| 语言: | English |
| 出版: |
Public Library of Science
2020
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| 在线阅读: | http://psasir.upm.edu.my/id/eprint/88662/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/88662/ https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0241074 |
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