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Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics

Genotype VII Newcastle disease viruses are associated with huge economic losses in the global poultry industry. Despite the intensive applications of vaccines, disease outbreaks caused by those viruses continue to occur frequently even among the vaccinated poultry farms. An important factor in the s...

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Main Authors: Bello, Muhammad Bashir, Mahamud, Siti Nor Azizah, Yusoff, Khatijah, Ideris, Aini, Bejo, Mohd Hair, Peeters, Ben P. H., Omar, Abdul Rahman
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88425/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/88425/
https://www.mdpi.com/2076-393X/8/2/270
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spelling my.upm.eprints.884252022-11-24T01:31:04Z http://psasir.upm.edu.my/id/eprint/88425/ Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics Bello, Muhammad Bashir Mahamud, Siti Nor Azizah Yusoff, Khatijah Ideris, Aini Bejo, Mohd Hair Peeters, Ben P. H. Omar, Abdul Rahman Genotype VII Newcastle disease viruses are associated with huge economic losses in the global poultry industry. Despite the intensive applications of vaccines, disease outbreaks caused by those viruses continue to occur frequently even among the vaccinated poultry farms. An important factor in the suboptimal protective efficacy of the current vaccines is the genetic mismatch between the prevalent strains and the vaccine strains. Therefore, in the present study, an effective and stable genotype-matched live attenuated Newcastle disease virus (NDV) vaccine was developed using reverse genetics, based on a recently isolated virulent naturally recombinant NDV IBS025/13 Malaysian strain. First of all, the sequence encoding the fusion protein (F) cleavage site of the virus was modified in silico from virulent polybasic (RRQKRF) to avirulent monobasic (GRQGRL) motif. The entire modified sequence was then chemically synthesized and inserted into pOLTV5 transcription vector for virus rescue. A recombinant virus termed mIBS025 was successfully recovered and shown to be highly attenuated based on OIE recommended pathogenicity assessment indices. Furthermore, the virus was shown to remain stably attenuated and retain the avirulent monobasic F cleavage site after 15 consecutive passages in specific-pathogen-free embryonated eggs and 12 passages in one-day-old chicks. More so, the recombinant virus induced a significantly higher hemagglutination inhibition antibody titre than LaSota although both vaccines fully protected chicken against genotype VII NDV induced mortality and morbidity. Finally, mIBS025 was shown to significantly reduce both the duration and quantity of cloacal and oropharyngeal shedding of the challenged genotype VII virus compared to the LaSota vaccine. These findings collectively indicate that mIBS025 provides a better protective efficacy than LaSota and therefore can be used as a promising vaccine candidate against genotype VII NDV strains. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88425/1/ABSTRACT.pdf Bello, Muhammad Bashir and Mahamud, Siti Nor Azizah and Yusoff, Khatijah and Ideris, Aini and Bejo, Mohd Hair and Peeters, Ben P. H. and Omar, Abdul Rahman (2020) Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics. Vaccines, 8 (2). art. no. 270. 14 - 17. ISSN 2076-393X https://www.mdpi.com/2076-393X/8/2/270 10.3390/vaccines8020270
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Genotype VII Newcastle disease viruses are associated with huge economic losses in the global poultry industry. Despite the intensive applications of vaccines, disease outbreaks caused by those viruses continue to occur frequently even among the vaccinated poultry farms. An important factor in the suboptimal protective efficacy of the current vaccines is the genetic mismatch between the prevalent strains and the vaccine strains. Therefore, in the present study, an effective and stable genotype-matched live attenuated Newcastle disease virus (NDV) vaccine was developed using reverse genetics, based on a recently isolated virulent naturally recombinant NDV IBS025/13 Malaysian strain. First of all, the sequence encoding the fusion protein (F) cleavage site of the virus was modified in silico from virulent polybasic (RRQKRF) to avirulent monobasic (GRQGRL) motif. The entire modified sequence was then chemically synthesized and inserted into pOLTV5 transcription vector for virus rescue. A recombinant virus termed mIBS025 was successfully recovered and shown to be highly attenuated based on OIE recommended pathogenicity assessment indices. Furthermore, the virus was shown to remain stably attenuated and retain the avirulent monobasic F cleavage site after 15 consecutive passages in specific-pathogen-free embryonated eggs and 12 passages in one-day-old chicks. More so, the recombinant virus induced a significantly higher hemagglutination inhibition antibody titre than LaSota although both vaccines fully protected chicken against genotype VII NDV induced mortality and morbidity. Finally, mIBS025 was shown to significantly reduce both the duration and quantity of cloacal and oropharyngeal shedding of the challenged genotype VII virus compared to the LaSota vaccine. These findings collectively indicate that mIBS025 provides a better protective efficacy than LaSota and therefore can be used as a promising vaccine candidate against genotype VII NDV strains.
format Article
author Bello, Muhammad Bashir
Mahamud, Siti Nor Azizah
Yusoff, Khatijah
Ideris, Aini
Bejo, Mohd Hair
Peeters, Ben P. H.
Omar, Abdul Rahman
spellingShingle Bello, Muhammad Bashir
Mahamud, Siti Nor Azizah
Yusoff, Khatijah
Ideris, Aini
Bejo, Mohd Hair
Peeters, Ben P. H.
Omar, Abdul Rahman
Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics
author_facet Bello, Muhammad Bashir
Mahamud, Siti Nor Azizah
Yusoff, Khatijah
Ideris, Aini
Bejo, Mohd Hair
Peeters, Ben P. H.
Omar, Abdul Rahman
author_sort Bello, Muhammad Bashir
title Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics
title_short Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics
title_full Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics
title_fullStr Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics
title_full_unstemmed Development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant Malaysian isolate using reverse genetics
title_sort development of an effective and stable genotype-matched live attenuated newcastle disease virus vaccine based on a novel naturally recombinant malaysian isolate using reverse genetics
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/88425/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/88425/
https://www.mdpi.com/2076-393X/8/2/270
_version_ 1751538224074850304
score 13.211869

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