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Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review

5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and en...

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Main Authors: Semail, Nadhiratul farihin, Abdul Keyon, Aemi Syazwani, Saad, Bahruddin, Noordin, Siti Salmah, Nik Mohamed Kamal, Nik Nur Syazni, Mohamad Zain, Nur Nadhirah, Azizi, Juzaili, Kamaruzaman, Sazlinda, Yahaya, Noorfatimah
Format: Article
Language:English
Published: Taylor and Francis 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87877/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/87877/
https://www.tandfonline.com/doi/abs/10.1080/10826076.2020.1781654
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spelling my.upm.eprints.878772022-06-01T00:56:52Z http://psasir.upm.edu.my/id/eprint/87877/ Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review Semail, Nadhiratul farihin Abdul Keyon, Aemi Syazwani Saad, Bahruddin Noordin, Siti Salmah Nik Mohamed Kamal, Nik Nur Syazni Mohamad Zain, Nur Nadhirah Azizi, Juzaili Kamaruzaman, Sazlinda Yahaya, Noorfatimah 5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and encourages fundamental investigations of this medication. The development of accurate and reliable sample preparation, as well as analytical methods, is critical to isolate targeted analytes from complex matrices, apart from increasing detection sensitivity of analytes. With that, this paper presents a review of prior studies pertaining to chromatographic and electrophoretic methods that focused on the analysis of 5-FU and its metabolites in biological matrices such as plasma and urine. This paper concentrates on HPLC, GC and CE systems, which are the most commonly used strategies for analytical separation of 5-FU and its metabolites from samples. Detection of these antineoplastic agents at trace level demands highly sensitive and selective analytical methodologies. Application of these analytical techniques to biological matrices is reviewed with a focus on method development strategies, including types of mobile phases and background electrolytes employed in LC and CE systems. Taylor and Francis 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87877/1/ABSTRACT.pdf Semail, Nadhiratul farihin and Abdul Keyon, Aemi Syazwani and Saad, Bahruddin and Noordin, Siti Salmah and Nik Mohamed Kamal, Nik Nur Syazni and Mohamad Zain, Nur Nadhirah and Azizi, Juzaili and Kamaruzaman, Sazlinda and Yahaya, Noorfatimah (2020) Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review. Journal of Liquid Chromatography and Related Technologies, 43 (15-16). 562 - 579. ISSN 1082-6076; ESSN: 1520-572X https://www.tandfonline.com/doi/abs/10.1080/10826076.2020.1781654 10.1080/10826076.2020.1781654
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description 5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and encourages fundamental investigations of this medication. The development of accurate and reliable sample preparation, as well as analytical methods, is critical to isolate targeted analytes from complex matrices, apart from increasing detection sensitivity of analytes. With that, this paper presents a review of prior studies pertaining to chromatographic and electrophoretic methods that focused on the analysis of 5-FU and its metabolites in biological matrices such as plasma and urine. This paper concentrates on HPLC, GC and CE systems, which are the most commonly used strategies for analytical separation of 5-FU and its metabolites from samples. Detection of these antineoplastic agents at trace level demands highly sensitive and selective analytical methodologies. Application of these analytical techniques to biological matrices is reviewed with a focus on method development strategies, including types of mobile phases and background electrolytes employed in LC and CE systems.
format Article
author Semail, Nadhiratul farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Noordin, Siti Salmah
Nik Mohamed Kamal, Nik Nur Syazni
Mohamad Zain, Nur Nadhirah
Azizi, Juzaili
Kamaruzaman, Sazlinda
Yahaya, Noorfatimah
spellingShingle Semail, Nadhiratul farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Noordin, Siti Salmah
Nik Mohamed Kamal, Nik Nur Syazni
Mohamad Zain, Nur Nadhirah
Azizi, Juzaili
Kamaruzaman, Sazlinda
Yahaya, Noorfatimah
Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
author_facet Semail, Nadhiratul farihin
Abdul Keyon, Aemi Syazwani
Saad, Bahruddin
Noordin, Siti Salmah
Nik Mohamed Kamal, Nik Nur Syazni
Mohamad Zain, Nur Nadhirah
Azizi, Juzaili
Kamaruzaman, Sazlinda
Yahaya, Noorfatimah
author_sort Semail, Nadhiratul farihin
title Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
title_short Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
title_full Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
title_fullStr Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
title_full_unstemmed Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
title_sort analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
publisher Taylor and Francis
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87877/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/87877/
https://www.tandfonline.com/doi/abs/10.1080/10826076.2020.1781654
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score 13.211869

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