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A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol

A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilizati...

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Main Authors: Mat Zaid, Mohd Hazani, Abdullah, Jaafar, Rozi, Normazida, Mohamad Rozlan, Aliff Aiman, Abu Hanifah, Sharina
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87683/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/87683/
https://www.mdpi.com/2079-4991/10/7/1346
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spelling my.upm.eprints.876832022-07-06T03:48:20Z http://psasir.upm.edu.my/id/eprint/87683/ A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol Mat Zaid, Mohd Hazani Abdullah, Jaafar Rozi, Normazida Mohamad Rozlan, Aliff Aiman Abu Hanifah, Sharina A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10−7 to 1.0 × 10 −12 M, with a detection limit of 0.5 × 10−12 M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87683/1/ABSTRACT.pdf Mat Zaid, Mohd Hazani and Abdullah, Jaafar and Rozi, Normazida and Mohamad Rozlan, Aliff Aiman and Abu Hanifah, Sharina (2020) A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol. Nanomaterials, 10 (7). pp. 1-14. ISSN 2079-4991 https://www.mdpi.com/2079-4991/10/7/1346 10.3390/nano10071346
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10−7 to 1.0 × 10 −12 M, with a detection limit of 0.5 × 10−12 M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples.
format Article
author Mat Zaid, Mohd Hazani
Abdullah, Jaafar
Rozi, Normazida
Mohamad Rozlan, Aliff Aiman
Abu Hanifah, Sharina
spellingShingle Mat Zaid, Mohd Hazani
Abdullah, Jaafar
Rozi, Normazida
Mohamad Rozlan, Aliff Aiman
Abu Hanifah, Sharina
A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol
author_facet Mat Zaid, Mohd Hazani
Abdullah, Jaafar
Rozi, Normazida
Mohamad Rozlan, Aliff Aiman
Abu Hanifah, Sharina
author_sort Mat Zaid, Mohd Hazani
title A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol
title_short A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol
title_full A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol
title_fullStr A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol
title_full_unstemmed A sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-Estradiol
title_sort sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ß-estradiol
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87683/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/87683/
https://www.mdpi.com/2079-4991/10/7/1346
_version_ 1738511966771609600
score 13.211869

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