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A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology?

Aims: To correlate the HPV genotypes with recurrence of disease and malignant transformation. Methods: A prevalence cross-sectional study. The tumour tissue was isolated from the paraffin-embedded tissue (PET). The DNA was extracted from the tissue using the QiAamp DNA Mini Kit (Qiagen, Germany). Ge...

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Main Authors: Husain, Salina, Ishak, Noor Liza, Gendeh, Balwant, Syed Husain Shahabuddin, Sharifah Noor Akmal
Format: Article
Language:English
Published: Wiley 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87589/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/87589/
https://www.authorea.com/users/310539/articles/441386-a-cross-sectional-study-on-sinonasal-inverted-papilloma-does-human-papilloma-virus-play-a-role-in-its-etiology
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spelling my.upm.eprints.875892022-07-06T07:47:53Z http://psasir.upm.edu.my/id/eprint/87589/ A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology? Husain, Salina Ishak, Noor Liza Gendeh, Balwant Syed Husain Shahabuddin, Sharifah Noor Akmal Aims: To correlate the HPV genotypes with recurrence of disease and malignant transformation. Methods: A prevalence cross-sectional study. The tumour tissue was isolated from the paraffin-embedded tissue (PET). The DNA was extracted from the tissue using the QiAamp DNA Mini Kit (Qiagen, Germany). Gel electrophoresis was performed to determine the presence of genomic DNA. HPV detection and genotyping were carried out using SACACE HPV High Risk and Low Risk Typing Real-TM kit (SACACE, Italy). Two different types of kits were used, that is HPV 6,11 Real-TM and HPV 16,18 Real-TM kits. Results: A total of 44 patients, 36 were male and 8 were female with a ratio of 5:1. 61.4% was Malay, 22.7% was Chinese, 11.4% Indian 4.5% other races. 15 out of 44 patients had HPV positive (34%). The recurrence rate of positive HPV infection compared to negative HPV was not statistically significant (p>0.05). There was a significant correlation of HPV 16 and 18 infection with malignant transformation (p<0.05). A high detection rate of a high-risk HPV type (67%) was observed in patients with inverted papilloma with malignant transformation. Conclusion: The prevalence of HPV in inverted papilloma is 34%. Our result supports that HPV infection is an aetiological factor in sinonasal inverted papilloma. A high-risk HPV plays a role in the oncogenesis of sinonasal inverted papilloma. Further studies should be conducted to further elaborate this relationship. Wiley 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87589/1/ABSTRACT.pdf Husain, Salina and Ishak, Noor Liza and Gendeh, Balwant and Syed Husain Shahabuddin, Sharifah Noor Akmal (2020) A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology? Authorea. pp. 1-6. ISSN 2373-146X https://www.authorea.com/users/310539/articles/441386-a-cross-sectional-study-on-sinonasal-inverted-papilloma-does-human-papilloma-virus-play-a-role-in-its-etiology 10.22541/au.158678550.00655404
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Aims: To correlate the HPV genotypes with recurrence of disease and malignant transformation. Methods: A prevalence cross-sectional study. The tumour tissue was isolated from the paraffin-embedded tissue (PET). The DNA was extracted from the tissue using the QiAamp DNA Mini Kit (Qiagen, Germany). Gel electrophoresis was performed to determine the presence of genomic DNA. HPV detection and genotyping were carried out using SACACE HPV High Risk and Low Risk Typing Real-TM kit (SACACE, Italy). Two different types of kits were used, that is HPV 6,11 Real-TM and HPV 16,18 Real-TM kits. Results: A total of 44 patients, 36 were male and 8 were female with a ratio of 5:1. 61.4% was Malay, 22.7% was Chinese, 11.4% Indian 4.5% other races. 15 out of 44 patients had HPV positive (34%). The recurrence rate of positive HPV infection compared to negative HPV was not statistically significant (p>0.05). There was a significant correlation of HPV 16 and 18 infection with malignant transformation (p<0.05). A high detection rate of a high-risk HPV type (67%) was observed in patients with inverted papilloma with malignant transformation. Conclusion: The prevalence of HPV in inverted papilloma is 34%. Our result supports that HPV infection is an aetiological factor in sinonasal inverted papilloma. A high-risk HPV plays a role in the oncogenesis of sinonasal inverted papilloma. Further studies should be conducted to further elaborate this relationship.
format Article
author Husain, Salina
Ishak, Noor Liza
Gendeh, Balwant
Syed Husain Shahabuddin, Sharifah Noor Akmal
spellingShingle Husain, Salina
Ishak, Noor Liza
Gendeh, Balwant
Syed Husain Shahabuddin, Sharifah Noor Akmal
A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology?
author_facet Husain, Salina
Ishak, Noor Liza
Gendeh, Balwant
Syed Husain Shahabuddin, Sharifah Noor Akmal
author_sort Husain, Salina
title A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology?
title_short A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology?
title_full A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology?
title_fullStr A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology?
title_full_unstemmed A cross-sectional study on Sinonasal inverted Papilloma: does human Papilloma virus play a role in its etiology?
title_sort cross-sectional study on sinonasal inverted papilloma: does human papilloma virus play a role in its etiology?
publisher Wiley
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87589/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/87589/
https://www.authorea.com/users/310539/articles/441386-a-cross-sectional-study-on-sinonasal-inverted-papilloma-does-human-papilloma-virus-play-a-role-in-its-etiology
_version_ 1738511958221520896
score 13.211869

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