Staff View: Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker

Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in...

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Main Authors:	Mohd Zuki, Zakiah, Yusop, Mohd Rafii, Ramli, Asfaliza, Yusuff, Oladosu, Latif, Mohammad Abdul, Sijam, Kamaruzaman, Ismail, Mohd Razi, Mohd Sarif, Hamidah
Format:	Article
Published:	Instituto de Investigaciones Agropecuarias 2020
Online Access:	http://psasir.upm.edu.my/id/eprint/87547/ https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200227
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spelling	my.upm.eprints.875472022-11-23T03:15:06Z http://psasir.upm.edu.my/id/eprint/87547/ Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker Mohd Zuki, Zakiah Yusop, Mohd Rafii Ramli, Asfaliza Yusuff, Oladosu Latif, Mohammad Abdul Sijam, Kamaruzaman Ismail, Mohd Razi Mohd Sarif, Hamidah Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in rice. Based on this background, this study was conducted to analyze segregation pattern of simple-sequence repeat (SSR) markers associated with BLB resistance genes in an F2 bulk population derived from a resistant variety (IRBB60) and a susceptible variety (MR219). Out of 129 simple sequence repeat (SSR) markers screened, 18 distinct polymorphism markers including R-gene based markers were used to screen 345 F2 progenies for resistance to BLB. Among the polymorphic markers, the chi-square analyses showed that 15 SSR markers (i.e. RM13 (xa5), RM21 (Xa21), RM122 (xa5), RM153 (xa5), RM164 (Xa13), RM206 (Xa10), RM5509, RM20B, RM25, RM163, RM169, RM218, RM267, RM276, and RM334) had a segregation ratio of 1:2:1 for a single gene model (df = 2.0, p ≤ 0.05). For phenotypic ratio, the F2 population segregated in ratio 3:1 (R:S) for resistant and susceptible plants, respectively. This indicated that resistance to BLB caused by pathotype X. oryzae pv. oryzae (Xoo) in the ‘MR219’ × ‘IRBB60’ F2 population is controlled by single dominant genes. The result presented in this study will help breeders to further breeding research in rice by enabling selection based on the genotype rather than on the phenotype. Similarly, the markers reported in this study will serve as a valuable tool for marker-aided selection for BLB resistance gene. Instituto de Investigaciones Agropecuarias 2020-06 Article PeerReviewed Mohd Zuki, Zakiah and Yusop, Mohd Rafii and Ramli, Asfaliza and Yusuff, Oladosu and Latif, Mohammad Abdul and Sijam, Kamaruzaman and Ismail, Mohd Razi and Mohd Sarif, Hamidah (2020) Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker. Chilean Journal of Agricultural Research, 80 (2). 227 - 233. ISSN 0718-5839 https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200227 10.4067/S0718-58392020000200227
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in rice. Based on this background, this study was conducted to analyze segregation pattern of simple-sequence repeat (SSR) markers associated with BLB resistance genes in an F2 bulk population derived from a resistant variety (IRBB60) and a susceptible variety (MR219). Out of 129 simple sequence repeat (SSR) markers screened, 18 distinct polymorphism markers including R-gene based markers were used to screen 345 F2 progenies for resistance to BLB. Among the polymorphic markers, the chi-square analyses showed that 15 SSR markers (i.e. RM13 (xa5), RM21 (Xa21), RM122 (xa5), RM153 (xa5), RM164 (Xa13), RM206 (Xa10), RM5509, RM20B, RM25, RM163, RM169, RM218, RM267, RM276, and RM334) had a segregation ratio of 1:2:1 for a single gene model (df = 2.0, p ≤ 0.05). For phenotypic ratio, the F2 population segregated in ratio 3:1 (R:S) for resistant and susceptible plants, respectively. This indicated that resistance to BLB caused by pathotype X. oryzae pv. oryzae (Xoo) in the ‘MR219’ × ‘IRBB60’ F2 population is controlled by single dominant genes. The result presented in this study will help breeders to further breeding research in rice by enabling selection based on the genotype rather than on the phenotype. Similarly, the markers reported in this study will serve as a valuable tool for marker-aided selection for BLB resistance gene.
format	Article
author	Mohd Zuki, Zakiah Yusop, Mohd Rafii Ramli, Asfaliza Yusuff, Oladosu Latif, Mohammad Abdul Sijam, Kamaruzaman Ismail, Mohd Razi Mohd Sarif, Hamidah
spellingShingle	Mohd Zuki, Zakiah Yusop, Mohd Rafii Ramli, Asfaliza Yusuff, Oladosu Latif, Mohammad Abdul Sijam, Kamaruzaman Ismail, Mohd Razi Mohd Sarif, Hamidah Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
author_facet	Mohd Zuki, Zakiah Yusop, Mohd Rafii Ramli, Asfaliza Yusuff, Oladosu Latif, Mohammad Abdul Sijam, Kamaruzaman Ismail, Mohd Razi Mohd Sarif, Hamidah
author_sort	Mohd Zuki, Zakiah
title	Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_short	Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_full	Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_fullStr	Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_full_unstemmed	Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
title_sort	segregation analysis for bacterial leaf blight disease resistance genes in rice ‘mr219’ using ssr marker
publisher	Instituto de Investigaciones Agropecuarias
publishDate	2020
url	http://psasir.upm.edu.my/id/eprint/87547/ https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200227
_version_	1751538215548878848
score	13.211869

Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker

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