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Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring

Ectopic implantation of donor testis cell aggregates in recipient mice results in de novo formation or regeneration of testis tissue and, as such, provides a unique in vivo model for the study of testis development. However, currently the results are inconsistent and the efficiency of the model rema...

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Main Authors: Awang Junaidi, Awang Hazmi, Singh, Jaswant, Honaramooz, Ali
Format: Article
Published: CSIRO Publishing 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87430/
https://www.publish.csiro.au/rd/RD19043
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spelling my.upm.eprints.874302023-05-18T01:30:04Z http://psasir.upm.edu.my/id/eprint/87430/ Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring Awang Junaidi, Awang Hazmi Singh, Jaswant Honaramooz, Ali Ectopic implantation of donor testis cell aggregates in recipient mice results in de novo formation or regeneration of testis tissue and, as such, provides a unique in vivo model for the study of testis development. However, currently the results are inconsistent and the efficiency of the model remains low. This study was designed to: (1) examine several factors that can potentially improve the consistency and efficiency of this model and (2) explore the use of ultrasound biomicroscopy (UBM) for the non-invasive in vivo evaluation of implants. Testis cell aggregates, containing ~40% gonocytes, from 1-week-old donor piglets were implanted under the back skin of immunodeficient mice through skin incisions using gel matrices or through subcutaneous injection without using gel matrices. The addition of gel matrices led to inconsistent tissue development; gelatin had the greatest development, followed by collagen, whereas agarose resulted in poor development. The results also depended on the implanted cell numbers since implants with 100 × 106 cells were larger than those with 50 × 106 cells. The injection approach for cell implantation was less invasive and resulted in more consistent and efficient testis tissue development. UBM provided promising results as a means of non-invasive monitoring of implants. CSIRO Publishing 2020-02 Article PeerReviewed Awang Junaidi, Awang Hazmi and Singh, Jaswant and Honaramooz, Ali (2020) Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring. Reproduction, Fertility and Development, 32 (6). 594 - 609. ISSN 1031-3613; ESSN: 1448-5990 https://www.publish.csiro.au/rd/RD19043 10.1071/RD19043
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Ectopic implantation of donor testis cell aggregates in recipient mice results in de novo formation or regeneration of testis tissue and, as such, provides a unique in vivo model for the study of testis development. However, currently the results are inconsistent and the efficiency of the model remains low. This study was designed to: (1) examine several factors that can potentially improve the consistency and efficiency of this model and (2) explore the use of ultrasound biomicroscopy (UBM) for the non-invasive in vivo evaluation of implants. Testis cell aggregates, containing ~40% gonocytes, from 1-week-old donor piglets were implanted under the back skin of immunodeficient mice through skin incisions using gel matrices or through subcutaneous injection without using gel matrices. The addition of gel matrices led to inconsistent tissue development; gelatin had the greatest development, followed by collagen, whereas agarose resulted in poor development. The results also depended on the implanted cell numbers since implants with 100 × 106 cells were larger than those with 50 × 106 cells. The injection approach for cell implantation was less invasive and resulted in more consistent and efficient testis tissue development. UBM provided promising results as a means of non-invasive monitoring of implants.
format Article
author Awang Junaidi, Awang Hazmi
Singh, Jaswant
Honaramooz, Ali
spellingShingle Awang Junaidi, Awang Hazmi
Singh, Jaswant
Honaramooz, Ali
Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring
author_facet Awang Junaidi, Awang Hazmi
Singh, Jaswant
Honaramooz, Ali
author_sort Awang Junaidi, Awang Hazmi
title Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring
title_short Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring
title_full Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring
title_fullStr Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring
title_full_unstemmed Regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring
title_sort regeneration of testis tissue after ectopic implantation of porcine testis cell aggregates in mice: improved consistency of outcomes and in situ monitoring
publisher CSIRO Publishing
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87430/
https://www.publish.csiro.au/rd/RD19043
_version_ 1768009369021054976
score 13.211869

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