Staff View: Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis

Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis

Strongyloides stercoralis is the intestinal nematode in humans, and it infects millions of people worldwide but thrives in warm countries with poor sanitation conditions. Clinical manifestations of the infection may range from asymptomatic to chronic. Strongyloides cause hyperinfection syndrome and...

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Main Authors:	Al-Zihiry, Khalid Jameel Kadhim, Abdulhaleem, Noor, Atshan, Salman Sahab, Kadhim, Amal Jameel, Ibraheem, Zaid Osamah, Unyah, Ngah Zasmy
Format:	Article
Published:	Medknow Publications 2020
Online Access:	http://psasir.upm.edu.my/id/eprint/87376/ https://www.atmph.org/
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id	my.upm.eprints.87376
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spelling	my.upm.eprints.873762023-09-18T04:02:48Z http://psasir.upm.edu.my/id/eprint/87376/ Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis Al-Zihiry, Khalid Jameel Kadhim Abdulhaleem, Noor Atshan, Salman Sahab Kadhim, Amal Jameel Ibraheem, Zaid Osamah Unyah, Ngah Zasmy Strongyloides stercoralis is the intestinal nematode in humans, and it infects millions of people worldwide but thrives in warm countries with poor sanitation conditions. Clinical manifestations of the infection may range from asymptomatic to chronic. Strongyloides cause hyperinfection syndrome and dissemination in individuals with impaired cell-mediated immunity due to its ability to proliferate within the host that may increase the mortality rate up to 87%. The diagnosis of hyperinfection syndrome is difficult to establish and entails a high level of suspicion. The objective of the present study was to measure the expression level of C-type lectin gene coding to protein biomarker candidates from the excretory/secretory (ES) products of the infective filariform larva that can be used as diagnostic indicators for early hyperinfection syndrome in strongyloidiasis. An experimental study was carried out to induce hyperinfection of L3 larvae of S. ratti in experimentally immunosuppressed Wistar rats using prednisolone, a corticosteroid immunosuppressive drug. Prednisolone treatment resulted in a significant increase in the parasitic intensities. Relative semi-quantitative real-time PCR was performed to compare the expression level of the C-type lectin’s gene between treated and nontreated groups with this drug. C-type lectin gene showed significantly higher expression levels in the treated samples. The study concluded that C-type lectin expression level was successfully measured and could be used as a diagnostic biomarker during early hyperinfection syndrome in strongyloidiasis. Medknow Publications 2020-01 Article PeerReviewed Al-Zihiry, Khalid Jameel Kadhim and Abdulhaleem, Noor and Atshan, Salman Sahab and Kadhim, Amal Jameel and Ibraheem, Zaid Osamah and Unyah, Ngah Zasmy (2020) Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis. Annals of Tropical Medicine and Public Health, 23 (4). pp. 1-8. ISSN 1755-6783 https://www.atmph.org/ 10.36295/ASRO.2020.23414
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Strongyloides stercoralis is the intestinal nematode in humans, and it infects millions of people worldwide but thrives in warm countries with poor sanitation conditions. Clinical manifestations of the infection may range from asymptomatic to chronic. Strongyloides cause hyperinfection syndrome and dissemination in individuals with impaired cell-mediated immunity due to its ability to proliferate within the host that may increase the mortality rate up to 87%. The diagnosis of hyperinfection syndrome is difficult to establish and entails a high level of suspicion. The objective of the present study was to measure the expression level of C-type lectin gene coding to protein biomarker candidates from the excretory/secretory (ES) products of the infective filariform larva that can be used as diagnostic indicators for early hyperinfection syndrome in strongyloidiasis. An experimental study was carried out to induce hyperinfection of L3 larvae of S. ratti in experimentally immunosuppressed Wistar rats using prednisolone, a corticosteroid immunosuppressive drug. Prednisolone treatment resulted in a significant increase in the parasitic intensities. Relative semi-quantitative real-time PCR was performed to compare the expression level of the C-type lectin’s gene between treated and nontreated groups with this drug. C-type lectin gene showed significantly higher expression levels in the treated samples. The study concluded that C-type lectin expression level was successfully measured and could be used as a diagnostic biomarker during early hyperinfection syndrome in strongyloidiasis.
format	Article
author	Al-Zihiry, Khalid Jameel Kadhim Abdulhaleem, Noor Atshan, Salman Sahab Kadhim, Amal Jameel Ibraheem, Zaid Osamah Unyah, Ngah Zasmy
spellingShingle	Al-Zihiry, Khalid Jameel Kadhim Abdulhaleem, Noor Atshan, Salman Sahab Kadhim, Amal Jameel Ibraheem, Zaid Osamah Unyah, Ngah Zasmy Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis
author_facet	Al-Zihiry, Khalid Jameel Kadhim Abdulhaleem, Noor Atshan, Salman Sahab Kadhim, Amal Jameel Ibraheem, Zaid Osamah Unyah, Ngah Zasmy
author_sort	Al-Zihiry, Khalid Jameel Kadhim
title	Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis
title_short	Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis
title_full	Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis
title_fullStr	Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis
title_full_unstemmed	Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis
title_sort	quantification of c-type lectin gene expression during hyperinfection in strongyloidiasis
publisher	Medknow Publications
publishDate	2020
url	http://psasir.upm.edu.my/id/eprint/87376/ https://www.atmph.org/
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score	13.211869

Quantification of C-type lectin gene expression during hyperinfection in strongyloidiasis

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