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Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract

M. Candidum has been frequently used as a traditional medicine to treat various diseases such as diarrhea, dysentery, haemorrhoids, cuts and wounds, toothache, and stomach ache. This research was aimed to identify the activity of M. candidum acetone extract as an antibacterial, antioxidant, anticanc...

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Main Authors: Juwitaningsih, Tita, Jahro, Iis Siti, Dumariris, Ida, Hermawati, Elvira, Rukayadi, Yaya
Format: Article
Language:English
Published: Rasayan Journal 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87191/1/Phytochemical%2C%20antibacterial%2C%20antioxidant%20and%20anticancer%20activity%20study.pdf
http://psasir.upm.edu.my/id/eprint/87191/
http://rasayanjournal.co.in/archiveissue.php?issueid=20
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spelling my.upm.eprints.871912022-01-13T07:38:16Z http://psasir.upm.edu.my/id/eprint/87191/ Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract Juwitaningsih, Tita Jahro, Iis Siti Dumariris, Ida Hermawati, Elvira Rukayadi, Yaya M. Candidum has been frequently used as a traditional medicine to treat various diseases such as diarrhea, dysentery, haemorrhoids, cuts and wounds, toothache, and stomach ache. This research was aimed to identify the activity of M. candidum acetone extract as an antibacterial, antioxidant, anticancer and phytochemical. Antibacterial activity test was performed in vitro against each of the two Gram-positive and Gram-negative bacteria by paper disc diffusion method followed by determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The antioxidant activity of the extract was tested against 2,2-diphenyl1- picrylhydrazyl (DPPH), while the cytotoxic activity of the extract was evaluated against MCF-7 cells. Furthermore, the identification of secondary metabolite content was determined by 1H-NMR spectroscopy. Activity test results revealed that acetone extract of M.Candidum leaf was activite against four pathogenic bacteria, such as P. acne ATCC (27853), S. saprophyticus ATCC (49907), S. Mutans ATCV (35668), C. freundii ATCC 8090) with inhibition diameter of 5.70 ± 0.17 - 11.23 ± 0.23 with MIC values of 1250 - 2500 μg /mL and MBC between 1250 - > 5000 μg /mL. M.candidum acetone extract has antioxidant and cytotoxic activity with IC50 value = 22.4761 µg / mL and IC50 = 601.09 ug / mL respectively. Also, the results of phytochemical tests indicated that M. candidum acetone extract contained terpenoids and aromatic compounds. Rasayan Journal 2020-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87191/1/Phytochemical%2C%20antibacterial%2C%20antioxidant%20and%20anticancer%20activity%20study.pdf Juwitaningsih, Tita and Jahro, Iis Siti and Dumariris, Ida and Hermawati, Elvira and Rukayadi, Yaya (2020) Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract. Rasayan Journal of Chemistry, 13 (2). 1096 - 1103. ISSN 0974-1496; ESSN:0976-0083 http://rasayanjournal.co.in/archiveissue.php?issueid=20 10.31788/RJC.2020.1325614
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description M. Candidum has been frequently used as a traditional medicine to treat various diseases such as diarrhea, dysentery, haemorrhoids, cuts and wounds, toothache, and stomach ache. This research was aimed to identify the activity of M. candidum acetone extract as an antibacterial, antioxidant, anticancer and phytochemical. Antibacterial activity test was performed in vitro against each of the two Gram-positive and Gram-negative bacteria by paper disc diffusion method followed by determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The antioxidant activity of the extract was tested against 2,2-diphenyl1- picrylhydrazyl (DPPH), while the cytotoxic activity of the extract was evaluated against MCF-7 cells. Furthermore, the identification of secondary metabolite content was determined by 1H-NMR spectroscopy. Activity test results revealed that acetone extract of M.Candidum leaf was activite against four pathogenic bacteria, such as P. acne ATCC (27853), S. saprophyticus ATCC (49907), S. Mutans ATCV (35668), C. freundii ATCC 8090) with inhibition diameter of 5.70 ± 0.17 - 11.23 ± 0.23 with MIC values of 1250 - 2500 μg /mL and MBC between 1250 - > 5000 μg /mL. M.candidum acetone extract has antioxidant and cytotoxic activity with IC50 value = 22.4761 µg / mL and IC50 = 601.09 ug / mL respectively. Also, the results of phytochemical tests indicated that M. candidum acetone extract contained terpenoids and aromatic compounds.
format Article
author Juwitaningsih, Tita
Jahro, Iis Siti
Dumariris, Ida
Hermawati, Elvira
Rukayadi, Yaya
spellingShingle Juwitaningsih, Tita
Jahro, Iis Siti
Dumariris, Ida
Hermawati, Elvira
Rukayadi, Yaya
Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract
author_facet Juwitaningsih, Tita
Jahro, Iis Siti
Dumariris, Ida
Hermawati, Elvira
Rukayadi, Yaya
author_sort Juwitaningsih, Tita
title Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract
title_short Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract
title_full Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract
title_fullStr Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract
title_full_unstemmed Phytochemical, antibacterial, antioxidant and anticancer activity study of M. candidum leaf aceton extract
title_sort phytochemical, antibacterial, antioxidant and anticancer activity study of m. candidum leaf aceton extract
publisher Rasayan Journal
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87191/1/Phytochemical%2C%20antibacterial%2C%20antioxidant%20and%20anticancer%20activity%20study.pdf
http://psasir.upm.edu.my/id/eprint/87191/
http://rasayanjournal.co.in/archiveissue.php?issueid=20
_version_ 1724075492730994688
score 13.211869

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