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Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique

In this study, the phase behavior of medium-chain triglyceride (MCT)/surfactant/water containing gemcitabine (GEM) was investigated. Ternary phase diagrams (TPD) were constructed with different surfactants (Span 20, Span 60, Span 80, Tween 80) and a mixture of surfactant (Tween 80:Span 80, at rati...

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Main Authors: Wahgiman, Nadiatul Atiqah, Salim, Norazlinaliza, Abdul Rahman, Mohd Basyaruddin
Format: Article
Published: Malaysian Analytical Sciences Society 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87141/
https://mjas.analis.com.my/mjas/v24_n3/html/24_3_7.html
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spelling my.upm.eprints.871412023-01-25T08:42:36Z http://psasir.upm.edu.my/id/eprint/87141/ Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique Wahgiman, Nadiatul Atiqah Salim, Norazlinaliza Abdul Rahman, Mohd Basyaruddin In this study, the phase behavior of medium-chain triglyceride (MCT)/surfactant/water containing gemcitabine (GEM) was investigated. Ternary phase diagrams (TPD) were constructed with different surfactants (Span 20, Span 60, Span 80, Tween 80) and a mixture of surfactant (Tween 80:Span 80, at ratio 1:1). Two observations were recorded, isotropic and multilayer regions. The isotropic region was classified as a transparent, translucent or homogeneous mixture, while the multilayer region was classified as an opaque, milky or heterogeneous mixture. From the ternary phase diagrams constructed, it showed that MCT/(Tween 80: Span 80, ratio 1:1)/ GEM in deionized water system gave the largest isotropic region with the minimum usage of mixed surfactant, where 25% area of the isotropic region was formed with 1% of the usage of surfactant. The composition of F1 (selection based on the ternary phase diagram) was 0.02 % w/w of GEM, 19.98 % w/w of deionized water, 5.00 % w/w of Tween 80:Span 80 at ratio 1:1 and 75 % w/w of MCT. Nanoemulsion formulation was prepared by low energy emulsification method (i.e. phase inversion composition (PIC) technique). As the formulation was prepared in large scale with PIC technique, sodium chloride solution (0.1 M NaCl solution) was added to form a stable formulation as stabilizer. Effect of different volume of NaCl solution (0.5, 1.0 and 1.5 mL) added into formulation was investigated. The results showed that the particle size increased as the amount of NaCl increased, the effect of the amount of NaCl solution is significant towards particles size on F1. The F1 was stable in normal storage condition as it remained a homogeneous formulation against centrifugation test and it was also stable in storage stability test at different temperature (4, 25 and 45 °C) within three months. Malaysian Analytical Sciences Society 2020-06-09 Article PeerReviewed Wahgiman, Nadiatul Atiqah and Salim, Norazlinaliza and Abdul Rahman, Mohd Basyaruddin (2020) Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique. Malaysian Journal of Analytical Sciences, 24 (3). 363 - 372. ISSN 1394-2506 https://mjas.analis.com.my/mjas/v24_n3/html/24_3_7.html
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description In this study, the phase behavior of medium-chain triglyceride (MCT)/surfactant/water containing gemcitabine (GEM) was investigated. Ternary phase diagrams (TPD) were constructed with different surfactants (Span 20, Span 60, Span 80, Tween 80) and a mixture of surfactant (Tween 80:Span 80, at ratio 1:1). Two observations were recorded, isotropic and multilayer regions. The isotropic region was classified as a transparent, translucent or homogeneous mixture, while the multilayer region was classified as an opaque, milky or heterogeneous mixture. From the ternary phase diagrams constructed, it showed that MCT/(Tween 80: Span 80, ratio 1:1)/ GEM in deionized water system gave the largest isotropic region with the minimum usage of mixed surfactant, where 25% area of the isotropic region was formed with 1% of the usage of surfactant. The composition of F1 (selection based on the ternary phase diagram) was 0.02 % w/w of GEM, 19.98 % w/w of deionized water, 5.00 % w/w of Tween 80:Span 80 at ratio 1:1 and 75 % w/w of MCT. Nanoemulsion formulation was prepared by low energy emulsification method (i.e. phase inversion composition (PIC) technique). As the formulation was prepared in large scale with PIC technique, sodium chloride solution (0.1 M NaCl solution) was added to form a stable formulation as stabilizer. Effect of different volume of NaCl solution (0.5, 1.0 and 1.5 mL) added into formulation was investigated. The results showed that the particle size increased as the amount of NaCl increased, the effect of the amount of NaCl solution is significant towards particles size on F1. The F1 was stable in normal storage condition as it remained a homogeneous formulation against centrifugation test and it was also stable in storage stability test at different temperature (4, 25 and 45 °C) within three months.
format Article
author Wahgiman, Nadiatul Atiqah
Salim, Norazlinaliza
Abdul Rahman, Mohd Basyaruddin
spellingShingle Wahgiman, Nadiatul Atiqah
Salim, Norazlinaliza
Abdul Rahman, Mohd Basyaruddin
Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique
author_facet Wahgiman, Nadiatul Atiqah
Salim, Norazlinaliza
Abdul Rahman, Mohd Basyaruddin
author_sort Wahgiman, Nadiatul Atiqah
title Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique
title_short Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique
title_full Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique
title_fullStr Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique
title_full_unstemmed Phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique
title_sort phase behaviour study on medium-chain triglyceride/surfactant/water systems containing gemcitabine using phase inversion composition technique
publisher Malaysian Analytical Sciences Society
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87141/
https://mjas.analis.com.my/mjas/v24_n3/html/24_3_7.html
_version_ 1756061496964546560
score 13.211869

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