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Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits

Drought-responsive element binding (DREB) is involved in the regulation of stress-responsive gene expressions in plants through abscisic acid (ABA)-independent pathway. In this study, constitutive expression of oil palm (Elaeis guineensis) EgDREB1 driven by double strength cauliflower mosaic virus 3...

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Main Authors: Mohamad Azzeme, Azzreena, Abdullah, Siti Nor Akmar, Abdul Aziz, Maheran, Megat Wahab, Puteri Edaroyati
Format: Article
Language:English
Published: Institute of Experimental Botany. Czech Academy of Sciences 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87035/1/Overexpression%20of%20oil%20palm%20EgDREB1.pdf
http://psasir.upm.edu.my/id/eprint/87035/
https://bp.ueb.cas.cz/artkey/bpl-202001-0015_overexpression-of-oil-palm-egdreb1-in-tomato-decreased-fruit-size-and-produced-parthenocarpic-fruits.php
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spelling my.upm.eprints.870352022-01-13T02:15:22Z http://psasir.upm.edu.my/id/eprint/87035/ Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits Mohamad Azzeme, Azzreena Abdullah, Siti Nor Akmar Abdul Aziz, Maheran Megat Wahab, Puteri Edaroyati Drought-responsive element binding (DREB) is involved in the regulation of stress-responsive gene expressions in plants through abscisic acid (ABA)-independent pathway. In this study, constitutive expression of oil palm (Elaeis guineensis) EgDREB1 driven by double strength cauliflower mosaic virus 35S promoter in tomato (Solanum lycopersicum) reduced seed number, produced parthenocarpic fruits, changed morphology of leaves, and increased root biomass of transgenic plants. Early flowering and fruiting of the transgenic lines were observed in the culture vessels. EgDREB1 was specifically expressed in the fruits and its expression was not detected in vegetative tissues (leaves and roots). Altered expression of several endogenous tomato genes involved in the biosynthesis of phytohormones including jasmonic acid, ethylene, auxin, cytokinin, gibberellin (GA) and ABA were observed compared to wild type plants. The expression of AP2-like-ethylene transcription factor (LeAP2), allene oxide synthase (LeAOS), allene oxide cyclase (LeAOC), aminocyclopropane-1-carboxylic acid synthase (LeACS), 1-aminocyclopropane-1-carboxylate oxidase 1 (LeACO), auxin responsive factor 8 (LeARF8), auxin/indole-3-acetic acid (LeAux/IAA), cytokinin oxidase/dehydrogenase-like (LeSlCKX1), adenylate isopentenyltransferase (LeSlIPT1), gibberellin 2-oxidase 2 (LeGa2ox2), gibberellin 20-oxidase 4 (LeGa20ox4) and ABA-aldehyde oxidase (LeAAO) were different in fruits with reduced seed number compared to parthenocarphic fruits. These results suggest that their expression has significant effects on fruit development in transgenic tomato. EgDREB1 may mediate the expression of some of these genes as dehydration-responsive element binding (DRE) motif were found in their promoter sequences. These data indicate that the EgDREB1 controls fruit development in trsngenic plants by regulating the expression of hormone-associated genes. Institute of Experimental Botany. Czech Academy of Sciences 2020-01-27 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87035/1/Overexpression%20of%20oil%20palm%20EgDREB1.pdf Mohamad Azzeme, Azzreena and Abdullah, Siti Nor Akmar and Abdul Aziz, Maheran and Megat Wahab, Puteri Edaroyati (2020) Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits. Biologia Plantarum, 64. 58 - 67. ISSN 0006-3134; ESSN: 1573-8264 https://bp.ueb.cas.cz/artkey/bpl-202001-0015_overexpression-of-oil-palm-egdreb1-in-tomato-decreased-fruit-size-and-produced-parthenocarpic-fruits.php 10.32615/bp.2019.084
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Drought-responsive element binding (DREB) is involved in the regulation of stress-responsive gene expressions in plants through abscisic acid (ABA)-independent pathway. In this study, constitutive expression of oil palm (Elaeis guineensis) EgDREB1 driven by double strength cauliflower mosaic virus 35S promoter in tomato (Solanum lycopersicum) reduced seed number, produced parthenocarpic fruits, changed morphology of leaves, and increased root biomass of transgenic plants. Early flowering and fruiting of the transgenic lines were observed in the culture vessels. EgDREB1 was specifically expressed in the fruits and its expression was not detected in vegetative tissues (leaves and roots). Altered expression of several endogenous tomato genes involved in the biosynthesis of phytohormones including jasmonic acid, ethylene, auxin, cytokinin, gibberellin (GA) and ABA were observed compared to wild type plants. The expression of AP2-like-ethylene transcription factor (LeAP2), allene oxide synthase (LeAOS), allene oxide cyclase (LeAOC), aminocyclopropane-1-carboxylic acid synthase (LeACS), 1-aminocyclopropane-1-carboxylate oxidase 1 (LeACO), auxin responsive factor 8 (LeARF8), auxin/indole-3-acetic acid (LeAux/IAA), cytokinin oxidase/dehydrogenase-like (LeSlCKX1), adenylate isopentenyltransferase (LeSlIPT1), gibberellin 2-oxidase 2 (LeGa2ox2), gibberellin 20-oxidase 4 (LeGa20ox4) and ABA-aldehyde oxidase (LeAAO) were different in fruits with reduced seed number compared to parthenocarphic fruits. These results suggest that their expression has significant effects on fruit development in transgenic tomato. EgDREB1 may mediate the expression of some of these genes as dehydration-responsive element binding (DRE) motif were found in their promoter sequences. These data indicate that the EgDREB1 controls fruit development in trsngenic plants by regulating the expression of hormone-associated genes.
format Article
author Mohamad Azzeme, Azzreena
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Megat Wahab, Puteri Edaroyati
spellingShingle Mohamad Azzeme, Azzreena
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Megat Wahab, Puteri Edaroyati
Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits
author_facet Mohamad Azzeme, Azzreena
Abdullah, Siti Nor Akmar
Abdul Aziz, Maheran
Megat Wahab, Puteri Edaroyati
author_sort Mohamad Azzeme, Azzreena
title Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits
title_short Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits
title_full Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits
title_fullStr Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits
title_full_unstemmed Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits
title_sort overexpression of oil palm egdreb1 in tomato decreased fruit size and produced parthenocarpic fruits
publisher Institute of Experimental Botany. Czech Academy of Sciences
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87035/1/Overexpression%20of%20oil%20palm%20EgDREB1.pdf
http://psasir.upm.edu.my/id/eprint/87035/
https://bp.ueb.cas.cz/artkey/bpl-202001-0015_overexpression-of-oil-palm-egdreb1-in-tomato-decreased-fruit-size-and-produced-parthenocarpic-fruits.php
_version_ 1724075490005745664
score 13.211869

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