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Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons

Routine analytical methods are constrained in the speed of application, sample throughput and inability to determine the right bioavailable loading of pollutants. Microbial biosensor technology resolved these constraints by offering the most rapid, sensitive, reliable and cost-effective technology,...

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Main Authors: Ibrahim, Hussein, Abdulrasheed, Mansur, Umar, Ahmed Faruk, Lawal, Hafsat, Ramírez, Nicolás, Ahmad, Siti Aqlima
Format: Article
Language:English
Published: Universidad Autonoma Metropolitana-Iztapalapa 2020
Online Access:http://psasir.upm.edu.my/id/eprint/86997/1/Optimisation%20and%20dose%20responses%20of%20bioluminescent.pdf
http://psasir.upm.edu.my/id/eprint/86997/
http://rmiq.org/ojs311/index.php/rmiq/article/view/1274
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spelling my.upm.eprints.869972022-01-10T07:48:17Z http://psasir.upm.edu.my/id/eprint/86997/ Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons Ibrahim, Hussein Abdulrasheed, Mansur Umar, Ahmed Faruk Lawal, Hafsat Ramírez, Nicolás Ahmad, Siti Aqlima Routine analytical methods are constrained in the speed of application, sample throughput and inability to determine the right bioavailable loading of pollutants. Microbial biosensor technology resolved these constraints by offering the most rapid, sensitive, reliable and cost-effective technology, especially in a bioavailable context. This study describes the growth characterisation and optimisation of three different lux-marked biosensors and their induction bioassay, thus testing their responses to doses of target hydrocarbons (naphthalene, toluene, Isopropylbenzene) and solution of mixed hydrocarbons. These biosensors, Pseudomonas fluorescence HK44, Escherichia coli HMS174 and Pseudomonas putidaTVA8 harbours luxCDABE reporter genes coupled to induction by hydrocarbons. Biosensors harvested at optimal exponential phase and induced with hydrocarbon using the optimised assay conditions are highly sensitive and responsive to their inducers in a proportionate dose-dependent status. The established dose responses of these catabolic biosensors signify the prospect of extrapolation for estimating the genuine contamination loading of pollutants for environmental relevance. However, several factors may contribute to the quenching effect at high concentration of inducers. Robust responsiveness to mixed hydrocarbon solution has been also realised accentuating its feasibility in analysing of real environmental samples containing heterogenous pollutants. This study emphasises the suitability of bioluminescent bacterial biosensors for pollutants analysis and notably the detection of soluble bioavailable fractions of diverse hydrocarbons, hence, serves as a reliable bioindicator of hydrocarbon pollution in an environment. Even so, the real value of biosensors is for a suite of ecologically justified biosensors to be applied in complementary combinations with other focused analytical or chemical methods for broad and resourceful inference. Universidad Autonoma Metropolitana-Iztapalapa 2020-04-19 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86997/1/Optimisation%20and%20dose%20responses%20of%20bioluminescent.pdf Ibrahim, Hussein and Abdulrasheed, Mansur and Umar, Ahmed Faruk and Lawal, Hafsat and Ramírez, Nicolás and Ahmad, Siti Aqlima (2020) Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons. Revista Mexicana de Ingenieria Quimica, 19 (suppl. 1). 187 - 199. ISSN 2395-8472 http://rmiq.org/ojs311/index.php/rmiq/article/view/1274 10.24275/rmiq/Bio1274
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Routine analytical methods are constrained in the speed of application, sample throughput and inability to determine the right bioavailable loading of pollutants. Microbial biosensor technology resolved these constraints by offering the most rapid, sensitive, reliable and cost-effective technology, especially in a bioavailable context. This study describes the growth characterisation and optimisation of three different lux-marked biosensors and their induction bioassay, thus testing their responses to doses of target hydrocarbons (naphthalene, toluene, Isopropylbenzene) and solution of mixed hydrocarbons. These biosensors, Pseudomonas fluorescence HK44, Escherichia coli HMS174 and Pseudomonas putidaTVA8 harbours luxCDABE reporter genes coupled to induction by hydrocarbons. Biosensors harvested at optimal exponential phase and induced with hydrocarbon using the optimised assay conditions are highly sensitive and responsive to their inducers in a proportionate dose-dependent status. The established dose responses of these catabolic biosensors signify the prospect of extrapolation for estimating the genuine contamination loading of pollutants for environmental relevance. However, several factors may contribute to the quenching effect at high concentration of inducers. Robust responsiveness to mixed hydrocarbon solution has been also realised accentuating its feasibility in analysing of real environmental samples containing heterogenous pollutants. This study emphasises the suitability of bioluminescent bacterial biosensors for pollutants analysis and notably the detection of soluble bioavailable fractions of diverse hydrocarbons, hence, serves as a reliable bioindicator of hydrocarbon pollution in an environment. Even so, the real value of biosensors is for a suite of ecologically justified biosensors to be applied in complementary combinations with other focused analytical or chemical methods for broad and resourceful inference.
format Article
author Ibrahim, Hussein
Abdulrasheed, Mansur
Umar, Ahmed Faruk
Lawal, Hafsat
Ramírez, Nicolás
Ahmad, Siti Aqlima
spellingShingle Ibrahim, Hussein
Abdulrasheed, Mansur
Umar, Ahmed Faruk
Lawal, Hafsat
Ramírez, Nicolás
Ahmad, Siti Aqlima
Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons
author_facet Ibrahim, Hussein
Abdulrasheed, Mansur
Umar, Ahmed Faruk
Lawal, Hafsat
Ramírez, Nicolás
Ahmad, Siti Aqlima
author_sort Ibrahim, Hussein
title Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons
title_short Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons
title_full Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons
title_fullStr Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons
title_full_unstemmed Optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons
title_sort optimisation and dose responses of bioluminescent bacterial biosensors induced with target hydrocarbons
publisher Universidad Autonoma Metropolitana-Iztapalapa
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/86997/1/Optimisation%20and%20dose%20responses%20of%20bioluminescent.pdf
http://psasir.upm.edu.my/id/eprint/86997/
http://rmiq.org/ojs311/index.php/rmiq/article/view/1274
_version_ 1724075485498966016
score 13.211869

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