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Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis

Despite pulmonary cryptococcosis showing good response to antifungal treatment, making accurate diagnosis in a timely manner remains a challenge. Tissue for fungal culture is less sensitive, nonspecific, and time-consuming to allow for prompt diagnosis. We herein report a case of 31-year-old woman...

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Main Authors: Mohd Rahim, Syarifah, Salleh, Amizatul Aini, Bakrin, Ikmal Hisyam, Tengku Jamaluddin, Tengku Zetty Maztura, Masri, Siti Norbaya
Format: Article
Language:English
Published: Fakulti Perubatan dan Sains Kesihatan, Universiti Putra Malaysia 2020
Online Access:http://psasir.upm.edu.my/id/eprint/86813/1/Molecular%20Detection%20of%20Cryptococcus%20from%20Formalin.pdf
http://psasir.upm.edu.my/id/eprint/86813/
https://medic.upm.edu.my/upload/dokumen/2020091012202011_2019_0210.pdf
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spelling my.upm.eprints.868132021-11-17T10:40:01Z http://psasir.upm.edu.my/id/eprint/86813/ Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis Mohd Rahim, Syarifah Salleh, Amizatul Aini Bakrin, Ikmal Hisyam Tengku Jamaluddin, Tengku Zetty Maztura Masri, Siti Norbaya Despite pulmonary cryptococcosis showing good response to antifungal treatment, making accurate diagnosis in a timely manner remains a challenge. Tissue for fungal culture is less sensitive, nonspecific, and time-consuming to allow for prompt diagnosis. We herein report a case of 31-year-old woman with no known medical illness but presented with history of cough and haemoptysis for two months. The chest X-ray and bronchoscopy revealed left lung mass. While tuberculosis showed negative result and no growth observed in the tissue culture, the histopathological examination (HPE) finding was suggestive of fungal infection. The formalin-fixed paraffin-embedded (FFPE) tissue was sent for molecular testing, which revealed Cryptococcus neoformans. This report emphasises on the advantages and limitations of polymerase chain reaction (PCR) as an alternative method to confirm the diagnosis in cases of culture-negative fungal infection. Fakulti Perubatan dan Sains Kesihatan, Universiti Putra Malaysia 2020-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86813/1/Molecular%20Detection%20of%20Cryptococcus%20from%20Formalin.pdf Mohd Rahim, Syarifah and Salleh, Amizatul Aini and Bakrin, Ikmal Hisyam and Tengku Jamaluddin, Tengku Zetty Maztura and Masri, Siti Norbaya (2020) Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis. Malaysian Journal of Medicine and Health Sciences, 16 (SP1). 47 - 49. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/2020091012202011_2019_0210.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Despite pulmonary cryptococcosis showing good response to antifungal treatment, making accurate diagnosis in a timely manner remains a challenge. Tissue for fungal culture is less sensitive, nonspecific, and time-consuming to allow for prompt diagnosis. We herein report a case of 31-year-old woman with no known medical illness but presented with history of cough and haemoptysis for two months. The chest X-ray and bronchoscopy revealed left lung mass. While tuberculosis showed negative result and no growth observed in the tissue culture, the histopathological examination (HPE) finding was suggestive of fungal infection. The formalin-fixed paraffin-embedded (FFPE) tissue was sent for molecular testing, which revealed Cryptococcus neoformans. This report emphasises on the advantages and limitations of polymerase chain reaction (PCR) as an alternative method to confirm the diagnosis in cases of culture-negative fungal infection.
format Article
author Mohd Rahim, Syarifah
Salleh, Amizatul Aini
Bakrin, Ikmal Hisyam
Tengku Jamaluddin, Tengku Zetty Maztura
Masri, Siti Norbaya
spellingShingle Mohd Rahim, Syarifah
Salleh, Amizatul Aini
Bakrin, Ikmal Hisyam
Tengku Jamaluddin, Tengku Zetty Maztura
Masri, Siti Norbaya
Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis
author_facet Mohd Rahim, Syarifah
Salleh, Amizatul Aini
Bakrin, Ikmal Hisyam
Tengku Jamaluddin, Tengku Zetty Maztura
Masri, Siti Norbaya
author_sort Mohd Rahim, Syarifah
title Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis
title_short Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis
title_full Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis
title_fullStr Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis
title_full_unstemmed Molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis
title_sort molecular detection of cryptococcus from formalin-fixed paraffin-embedded tissue sample - a noncultural approach for diagnosis of primary pulmonary cryptococcosis
publisher Fakulti Perubatan dan Sains Kesihatan, Universiti Putra Malaysia
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/86813/1/Molecular%20Detection%20of%20Cryptococcus%20from%20Formalin.pdf
http://psasir.upm.edu.my/id/eprint/86813/
https://medic.upm.edu.my/upload/dokumen/2020091012202011_2019_0210.pdf
_version_ 1717095393602306048
score 13.211869

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