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Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats

The present study aimed to determine the effect of an ethyl acetate extract of Mikania micrantha stems (EAMMS) in hypercholesterolemia-induced rats. Rats were divided into a normal group (NC) and hypercholesterolemia induced groups: hypercholesterolemia control group (PC), simvastatin group (SV) (10...

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Main Authors: Ibrahim, Azlinda, Shafie, Nurul Husna, Mohd Esa, Norhaizan, Shafie, Siti Raihanah, Bahari, Hasnah, Abdullah, Maizaton Atmadini
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Online Access:http://psasir.upm.edu.my/id/eprint/86762/1/Mikania%20micrantha.pdf
http://psasir.upm.edu.my/id/eprint/86762/
https://www.mdpi.com/2072-6643/12/10/3077
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spelling my.upm.eprints.867622021-11-16T03:25:22Z http://psasir.upm.edu.my/id/eprint/86762/ Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats Ibrahim, Azlinda Shafie, Nurul Husna Mohd Esa, Norhaizan Shafie, Siti Raihanah Bahari, Hasnah Abdullah, Maizaton Atmadini The present study aimed to determine the effect of an ethyl acetate extract of Mikania micrantha stems (EAMMS) in hypercholesterolemia-induced rats. Rats were divided into a normal group (NC) and hypercholesterolemia induced groups: hypercholesterolemia control group (PC), simvastatin group (SV) (10 mg/kg) and EAMMS extract groups at different dosages of 50, 100 and 200 mg/kg, respectively. Blood serum and tissues were collected for haematological, biochemical, histopathological, and enzyme analysis. Total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, malondialdehyde (MDA) level, as well as enzymes of HMG-CoA reductase (HMGCR) and acetyl-CoA acetyltransferase 2 (ACAT2), were measured. Feeding rats with high cholesterol diet for eight weeks resulted in a significantly (p < 0.05) increased of TC, TG, LDL-C, AST, ALT and MDA levels. Meanwhile, the administration of EAMMS extract (50, 100 and 200 mg/kg) and simvastatin (10 mg/kg) significantly reduced (p < 0.05) the levels of TC, TG, LDL-C and MDA compared to rats in the PC group. Furthermore, all EAMMS and SV-treated groups showed a higher HDL-C level compared to both NC and PC groups. No significant difference was found in the level of ALT, AST, urea and creatinine between the different dosages in EAMMS extracts. Treatment with EAMMS also exhibited the highest inhibition activity of enzyme HMGCR and ACAT2 as compared to the control group. From the histopathological examination, liver tissues in the PC group showed severe steatosis than those fed with EAMMS and normal diet. Treatment with EAMMS extract ameliorated and reduced the pathological changes in the liver. No morphological changes showed in the kidney structure of both control and treated groups. In conclusion, these findings demonstrated that EAMMS extract has anti-hypercholesterolemia properties and could be used as an alternative treatment for this disorder. Multidisciplinary Digital Publishing Institute 2020-10-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86762/1/Mikania%20micrantha.pdf Ibrahim, Azlinda and Shafie, Nurul Husna and Mohd Esa, Norhaizan and Shafie, Siti Raihanah and Bahari, Hasnah and Abdullah, Maizaton Atmadini (2020) Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats. Nutrients, 12 (10). pp. 1-16. ISSN 2072-6643 https://www.mdpi.com/2072-6643/12/10/3077 10.3390/nu12103077
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The present study aimed to determine the effect of an ethyl acetate extract of Mikania micrantha stems (EAMMS) in hypercholesterolemia-induced rats. Rats were divided into a normal group (NC) and hypercholesterolemia induced groups: hypercholesterolemia control group (PC), simvastatin group (SV) (10 mg/kg) and EAMMS extract groups at different dosages of 50, 100 and 200 mg/kg, respectively. Blood serum and tissues were collected for haematological, biochemical, histopathological, and enzyme analysis. Total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, malondialdehyde (MDA) level, as well as enzymes of HMG-CoA reductase (HMGCR) and acetyl-CoA acetyltransferase 2 (ACAT2), were measured. Feeding rats with high cholesterol diet for eight weeks resulted in a significantly (p < 0.05) increased of TC, TG, LDL-C, AST, ALT and MDA levels. Meanwhile, the administration of EAMMS extract (50, 100 and 200 mg/kg) and simvastatin (10 mg/kg) significantly reduced (p < 0.05) the levels of TC, TG, LDL-C and MDA compared to rats in the PC group. Furthermore, all EAMMS and SV-treated groups showed a higher HDL-C level compared to both NC and PC groups. No significant difference was found in the level of ALT, AST, urea and creatinine between the different dosages in EAMMS extracts. Treatment with EAMMS also exhibited the highest inhibition activity of enzyme HMGCR and ACAT2 as compared to the control group. From the histopathological examination, liver tissues in the PC group showed severe steatosis than those fed with EAMMS and normal diet. Treatment with EAMMS extract ameliorated and reduced the pathological changes in the liver. No morphological changes showed in the kidney structure of both control and treated groups. In conclusion, these findings demonstrated that EAMMS extract has anti-hypercholesterolemia properties and could be used as an alternative treatment for this disorder.
format Article
author Ibrahim, Azlinda
Shafie, Nurul Husna
Mohd Esa, Norhaizan
Shafie, Siti Raihanah
Bahari, Hasnah
Abdullah, Maizaton Atmadini
spellingShingle Ibrahim, Azlinda
Shafie, Nurul Husna
Mohd Esa, Norhaizan
Shafie, Siti Raihanah
Bahari, Hasnah
Abdullah, Maizaton Atmadini
Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats
author_facet Ibrahim, Azlinda
Shafie, Nurul Husna
Mohd Esa, Norhaizan
Shafie, Siti Raihanah
Bahari, Hasnah
Abdullah, Maizaton Atmadini
author_sort Ibrahim, Azlinda
title Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats
title_short Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats
title_full Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats
title_fullStr Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats
title_full_unstemmed Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats
title_sort mikania micrantha extract inhibits hmg-coa reductase and acat2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/86762/1/Mikania%20micrantha.pdf
http://psasir.upm.edu.my/id/eprint/86762/
https://www.mdpi.com/2072-6643/12/10/3077
_version_ 1717095387340210176
score 13.211869

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