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Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods

The diversity of soil microbial communities at Barrientos Island with differents soil characteristics were evaluated using PCR-based method random amplified polymorphic DNA (RAPD) and community level physiological profiles (CLPP) of Biolog Ecoplate. The soils were selected from 17 different locatio...

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Main Authors: Lee, Learn Han, Letchumanan, Vengadesh, Ab Mutalib, Nurul Syakima, Cheah, Yoke Kqueen
Format: Article
Language:English
Published: HH Publisher 2020
Online Access:http://psasir.upm.edu.my/id/eprint/86748/1/Microbial%20community%20diversity%20in%20the%20soil%20of%20Barrientos%20Island%20.pdf
http://psasir.upm.edu.my/id/eprint/86748/
https://journals.hh-publisher.com/index.php/pmmb/article/view/111
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spelling my.upm.eprints.867482021-11-16T02:49:55Z http://psasir.upm.edu.my/id/eprint/86748/ Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods Lee, Learn Han Letchumanan, Vengadesh Ab Mutalib, Nurul Syakima Cheah, Yoke Kqueen The diversity of soil microbial communities at Barrientos Island with differents soil characteristics were evaluated using PCR-based method random amplified polymorphic DNA (RAPD) and community level physiological profiles (CLPP) of Biolog Ecoplate. The soils were selected from 17 different locations around Barrientos Island inhabited by different breeders. Shannon-Weaver index and multivariate analysis were performed to characterize variations of soil microbial communities. Both RAPD and CLPP methods exhibited that most soils with different type of rookery and characteristics could possibly affect the DNA sequence diversity and soil microbial diversity. The abandoned type of rookery had the highest Shannon-Weaver index as exhibited by soil sample 445 (3.4 for RAPD) and 450 (3.09 for CLPP). Higher coefficients of DNA sequence similarity were found in soil samples colonized by similar breeders, like soil 442 and 446 (both were active Chinstrap rookery) shared highest similarity in DNA sequences (73.53). The cluster analysis of RAPD profiles by UPGMA and principle component analysis (PCA) of Biolog Ecoplate exhibited similar influence of type of rookery and soil condition towards soil microbial community diversity. The results may suggest that the change in microbial community DNA composition is accompanied with the change in microbial functional properties. HH Publisher 2020-09-17 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86748/1/Microbial%20community%20diversity%20in%20the%20soil%20of%20Barrientos%20Island%20.pdf Lee, Learn Han and Letchumanan, Vengadesh and Ab Mutalib, Nurul Syakima and Cheah, Yoke Kqueen (2020) Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods. Progress in Microbes and Molecular Biology, 3 (1). pp. 1-10. ISSN 2637-1049 https://journals.hh-publisher.com/index.php/pmmb/article/view/111 10.36877/pmmb.a0000046
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The diversity of soil microbial communities at Barrientos Island with differents soil characteristics were evaluated using PCR-based method random amplified polymorphic DNA (RAPD) and community level physiological profiles (CLPP) of Biolog Ecoplate. The soils were selected from 17 different locations around Barrientos Island inhabited by different breeders. Shannon-Weaver index and multivariate analysis were performed to characterize variations of soil microbial communities. Both RAPD and CLPP methods exhibited that most soils with different type of rookery and characteristics could possibly affect the DNA sequence diversity and soil microbial diversity. The abandoned type of rookery had the highest Shannon-Weaver index as exhibited by soil sample 445 (3.4 for RAPD) and 450 (3.09 for CLPP). Higher coefficients of DNA sequence similarity were found in soil samples colonized by similar breeders, like soil 442 and 446 (both were active Chinstrap rookery) shared highest similarity in DNA sequences (73.53). The cluster analysis of RAPD profiles by UPGMA and principle component analysis (PCA) of Biolog Ecoplate exhibited similar influence of type of rookery and soil condition towards soil microbial community diversity. The results may suggest that the change in microbial community DNA composition is accompanied with the change in microbial functional properties.
format Article
author Lee, Learn Han
Letchumanan, Vengadesh
Ab Mutalib, Nurul Syakima
Cheah, Yoke Kqueen
spellingShingle Lee, Learn Han
Letchumanan, Vengadesh
Ab Mutalib, Nurul Syakima
Cheah, Yoke Kqueen
Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods
author_facet Lee, Learn Han
Letchumanan, Vengadesh
Ab Mutalib, Nurul Syakima
Cheah, Yoke Kqueen
author_sort Lee, Learn Han
title Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods
title_short Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods
title_full Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods
title_fullStr Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods
title_full_unstemmed Microbial community diversity in the soil of Barrientos Island estimated by RAPD and Biolog Ecoplate methods
title_sort microbial community diversity in the soil of barrientos island estimated by rapd and biolog ecoplate methods
publisher HH Publisher
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/86748/1/Microbial%20community%20diversity%20in%20the%20soil%20of%20Barrientos%20Island%20.pdf
http://psasir.upm.edu.my/id/eprint/86748/
https://journals.hh-publisher.com/index.php/pmmb/article/view/111
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score 13.211869

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