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Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes

The deposition of paraffin wax in crude oil is a problem faced by the oil and gas industry during extraction, transportation, and refining of crude oil. Most of the commercialized chemical additives to prevent wax are expensive and toxic. As an environmentally friendly alternative, this study aims t...

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Main Authors: Adlan, Nur Aina, Sabri, Suriana, Masomian, Malihe, Mohamad Ali, Mohd Shukuri, Raja Abdul Rahman, Raja Noor Zaliha
Format: Article
Language:English
Published: Frontiers Media 2020
Online Access:http://psasir.upm.edu.my/id/eprint/86747/1/Microbial%20biodegradation%20of%20paraffin%20wax%20in%20Malaysian%20crude%20oil%20mediated%20by%20degradative%20enzymes.pdf
http://psasir.upm.edu.my/id/eprint/86747/
https://www.frontiersin.org/articles/10.3389/fmicb.2020.565608/full
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spelling my.upm.eprints.867472021-11-16T02:43:45Z http://psasir.upm.edu.my/id/eprint/86747/ Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes Adlan, Nur Aina Sabri, Suriana Masomian, Malihe Mohamad Ali, Mohd Shukuri Raja Abdul Rahman, Raja Noor Zaliha The deposition of paraffin wax in crude oil is a problem faced by the oil and gas industry during extraction, transportation, and refining of crude oil. Most of the commercialized chemical additives to prevent wax are expensive and toxic. As an environmentally friendly alternative, this study aims to find a novel thermophilic bacterial strain capable of degrading paraffin wax in crude oil to control wax deposition. To achieve this, the biodegradation of crude oil paraffin wax by 11 bacteria isolated from seawater and oil-contaminated soil samples was investigated at 70°C. The bacteria were identified as Geobacillus kaustophilus N3A7, NFA23, DFY1, Geobacillus jurassicus MK7, Geobacillus thermocatenulatus T7, Parageobacillus caldoxylosilyticus DFY3 and AZ72, Anoxybacillus geothermalis D9, Geobacillus stearothermophilus SA36, AD11, and AD24. The GCMS analysis showed that strains N3A7, MK7, DFY1, AD11, and AD24 achieved more than 70% biodegradation efficiency of crude oil in a short period (3 days). Notably, most of the strains could completely degrade C37–C40 and increase the ratio of C14–C18, especially during the initial 2 days incubation. In addition, the degradation of crude oil also resulted in changes in the pH of the medium. The degradation of crude oil is associated with the production of degradative enzymes such as alkane monooxygenase, alcohol dehydrogenase, lipase, and esterase. Among the 11 strains, the highest activities of alkane monooxygenase were recorded in strain AD24. A comparatively higher overall alcohol dehydrogenase, lipase, and esterase activities were observed in strains N3A7, MK7, DFY1, AD11, and AD24. Thus, there is a potential to use these strains in oil reservoirs, crude oil processing, and recovery to control wax deposition. Their ability to withstand high temperature and produce degradative enzymes for long-chain hydrocarbon degradation led to an increase in the short-chain hydrocarbon ratio, and subsequently, improving the quality of the oil. Frontiers Media 2020-09-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86747/1/Microbial%20biodegradation%20of%20paraffin%20wax%20in%20Malaysian%20crude%20oil%20mediated%20by%20degradative%20enzymes.pdf Adlan, Nur Aina and Sabri, Suriana and Masomian, Malihe and Mohamad Ali, Mohd Shukuri and Raja Abdul Rahman, Raja Noor Zaliha (2020) Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes. Frontiers in Microbiology, 11. pp. 1-31. ISSN 1664-302X https://www.frontiersin.org/articles/10.3389/fmicb.2020.565608/full 10.3389/fmicb.2020.565608
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The deposition of paraffin wax in crude oil is a problem faced by the oil and gas industry during extraction, transportation, and refining of crude oil. Most of the commercialized chemical additives to prevent wax are expensive and toxic. As an environmentally friendly alternative, this study aims to find a novel thermophilic bacterial strain capable of degrading paraffin wax in crude oil to control wax deposition. To achieve this, the biodegradation of crude oil paraffin wax by 11 bacteria isolated from seawater and oil-contaminated soil samples was investigated at 70°C. The bacteria were identified as Geobacillus kaustophilus N3A7, NFA23, DFY1, Geobacillus jurassicus MK7, Geobacillus thermocatenulatus T7, Parageobacillus caldoxylosilyticus DFY3 and AZ72, Anoxybacillus geothermalis D9, Geobacillus stearothermophilus SA36, AD11, and AD24. The GCMS analysis showed that strains N3A7, MK7, DFY1, AD11, and AD24 achieved more than 70% biodegradation efficiency of crude oil in a short period (3 days). Notably, most of the strains could completely degrade C37–C40 and increase the ratio of C14–C18, especially during the initial 2 days incubation. In addition, the degradation of crude oil also resulted in changes in the pH of the medium. The degradation of crude oil is associated with the production of degradative enzymes such as alkane monooxygenase, alcohol dehydrogenase, lipase, and esterase. Among the 11 strains, the highest activities of alkane monooxygenase were recorded in strain AD24. A comparatively higher overall alcohol dehydrogenase, lipase, and esterase activities were observed in strains N3A7, MK7, DFY1, AD11, and AD24. Thus, there is a potential to use these strains in oil reservoirs, crude oil processing, and recovery to control wax deposition. Their ability to withstand high temperature and produce degradative enzymes for long-chain hydrocarbon degradation led to an increase in the short-chain hydrocarbon ratio, and subsequently, improving the quality of the oil.
format Article
author Adlan, Nur Aina
Sabri, Suriana
Masomian, Malihe
Mohamad Ali, Mohd Shukuri
Raja Abdul Rahman, Raja Noor Zaliha
spellingShingle Adlan, Nur Aina
Sabri, Suriana
Masomian, Malihe
Mohamad Ali, Mohd Shukuri
Raja Abdul Rahman, Raja Noor Zaliha
Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes
author_facet Adlan, Nur Aina
Sabri, Suriana
Masomian, Malihe
Mohamad Ali, Mohd Shukuri
Raja Abdul Rahman, Raja Noor Zaliha
author_sort Adlan, Nur Aina
title Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes
title_short Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes
title_full Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes
title_fullStr Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes
title_full_unstemmed Microbial biodegradation of paraffin wax in Malaysian crude oil mediated by degradative enzymes
title_sort microbial biodegradation of paraffin wax in malaysian crude oil mediated by degradative enzymes
publisher Frontiers Media
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/86747/1/Microbial%20biodegradation%20of%20paraffin%20wax%20in%20Malaysian%20crude%20oil%20mediated%20by%20degradative%20enzymes.pdf
http://psasir.upm.edu.my/id/eprint/86747/
https://www.frontiersin.org/articles/10.3389/fmicb.2020.565608/full
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score 13.211869

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