Stevia: limiting cholesterol synthesis in Hep-G2 cells
As of today, no literature has been reported on the efficacy of stevia on lipid regulations conducted in vitro. Thus, the current study was focusing on the potential of Stevia rebaudiana bertoni as an anti-hypercholesterolemia substitute in limiting the de novo cholesterol synthesis in Hep-G2 cell...
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| Main Authors: | , , , |
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| Format: | Article |
| Published: |
Malaysian Society for Molecular Biology and Biotechnology
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/85917/ http://www.msmbb.my/index.php/archive-issues/18-apjmbb/451-archive-issue-28 |
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| Summary: | As of today, no literature has been reported on the efficacy of stevia on lipid regulations
conducted in vitro. Thus, the current study was focusing on the potential of Stevia rebaudiana bertoni as an
anti-hypercholesterolemia substitute in limiting the de novo cholesterol synthesis in Hep-G2 cell line. The
cytotoxicity and lipid internalization effects of stevia on Hep-G2 cells were assessed quantitatively and
qualitatively in this study. As evaluated by MTT assay, commercialized stevia (0.5-20.0 mg/ml) and
stevioside (1.0-10 µM) inhibited Hep-G2 cells viability in a dose-dependent manner for 24 hours. IC50 was
detected at 8.68 mg/ml (commercialized stevia) and 10.91 µM (stevioside). From the assay, suitable
concentrations were chosen to study the effect of stevia on cholesterol internalization in Hep-G2 cells
supplemented with exogenous lipids. Cholesterol quantification assay revealed that high concentration
commercialized stevia and stevioside promoted significant cholesterol internalized in Hep-G2 cells as
compared to simvastatin. Finally, immunofluorescent microscopy assessment was done to qualitatively
observe the formation of lipid droplets and low-density lipoprotein receptor in relation to total cholesterol
extracted. The microphotographs of immunofluorescent microscopy were in parallel to results obtained
from the cholesterol quantification assay which further revealed the effect of stevia as a potential antihypercholesterolemia agent. |
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