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Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus)

Objective: To evaluate normal spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus). Methods: Four adult male crocodiles aged between 12-15 years, with an average snout to tail length of (3.15±0.01) m were selected for this study. Manipulation of the phallus d...

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Main Authors: Wan Nor Fitri, Haron, Wahid, Tengku Rinalfi Putra, Raj, Dana, Yawah, Donny, Latip, Qayyum, Ab Aziz, Abdul Malek
Format: Article
Published: Wolters Kluwer - Medknow Publications and Media 2020
Online Access:http://psasir.upm.edu.my/id/eprint/85882/
https://journals.lww.com/apjr/Fulltext/2020/09020/Spermatozoa_morphometry_and_ultrastructure_in.8.aspx#:~:text=Results%3A,(88.96%C2%B10.52)%20%CE%BCm.
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spelling my.upm.eprints.858822023-10-09T02:13:15Z http://psasir.upm.edu.my/id/eprint/85882/ Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus) Wan Nor Fitri Haron, Wahid Tengku Rinalfi Putra Raj, Dana Yawah, Donny Latip, Qayyum Ab Aziz, Abdul Malek Objective: To evaluate normal spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus). Methods: Four adult male crocodiles aged between 12-15 years, with an average snout to tail length of (3.15±0.01) m were selected for this study. Manipulation of the phallus digitally from the base of the penis was performed on four adult male crocodiles to facilitate the flow of semen to the sulcus. Semen was collected from all individuals for spermatozoa morphometry and ultrastructure study. Morphometry analysis was performed from eosin-nigrosin stained spermatozoa sample. Scanning electron microscopy was conducted to observe the surface ultrastructure of spermatozoa. Results: The morphology of crocodile spermatozoa was made up of acrosome, head, and tail which corresponded to (5.55±1.20) μm, (12.74±1.57) μm, and (70.67±4.40) μm, respectively. The total length of spermatozoa in estuarine crocodile was measured at (88.96±0.52) μm. The most common spermatozoa abnormalities found in the samples were cytoplasmic droplets. Conclusions: Normal morphology, morphometry of spermatozoa in estuarine crocodile which are vermiform shaped-head with a long tail along with questionably high cytoplasmic droplets count are described in this study. Wolters Kluwer - Medknow Publications and Media 2020 Article PeerReviewed Wan Nor Fitri and Haron, Wahid and Tengku Rinalfi Putra and Raj, Dana and Yawah, Donny and Latip, Qayyum and Ab Aziz, Abdul Malek (2020) Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus). Asian Pacific Journal of Reproduction, 9 (2). 104 - 108. ISSN 2305-0500; ESSN: 2305-0519 https://journals.lww.com/apjr/Fulltext/2020/09020/Spermatozoa_morphometry_and_ultrastructure_in.8.aspx#:~:text=Results%3A,(88.96%C2%B10.52)%20%CE%BCm. 10.4103/2305-0500.281080
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Objective: To evaluate normal spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus). Methods: Four adult male crocodiles aged between 12-15 years, with an average snout to tail length of (3.15±0.01) m were selected for this study. Manipulation of the phallus digitally from the base of the penis was performed on four adult male crocodiles to facilitate the flow of semen to the sulcus. Semen was collected from all individuals for spermatozoa morphometry and ultrastructure study. Morphometry analysis was performed from eosin-nigrosin stained spermatozoa sample. Scanning electron microscopy was conducted to observe the surface ultrastructure of spermatozoa. Results: The morphology of crocodile spermatozoa was made up of acrosome, head, and tail which corresponded to (5.55±1.20) μm, (12.74±1.57) μm, and (70.67±4.40) μm, respectively. The total length of spermatozoa in estuarine crocodile was measured at (88.96±0.52) μm. The most common spermatozoa abnormalities found in the samples were cytoplasmic droplets. Conclusions: Normal morphology, morphometry of spermatozoa in estuarine crocodile which are vermiform shaped-head with a long tail along with questionably high cytoplasmic droplets count are described in this study.
format Article
author Wan Nor Fitri
Haron, Wahid
Tengku Rinalfi Putra
Raj, Dana
Yawah, Donny
Latip, Qayyum
Ab Aziz, Abdul Malek
spellingShingle Wan Nor Fitri
Haron, Wahid
Tengku Rinalfi Putra
Raj, Dana
Yawah, Donny
Latip, Qayyum
Ab Aziz, Abdul Malek
Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus)
author_facet Wan Nor Fitri
Haron, Wahid
Tengku Rinalfi Putra
Raj, Dana
Yawah, Donny
Latip, Qayyum
Ab Aziz, Abdul Malek
author_sort Wan Nor Fitri
title Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus)
title_short Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus)
title_full Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus)
title_fullStr Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus)
title_full_unstemmed Spermatozoa morphometry and ultrastructure in estuarine crocodile (Crocodylus porosus)
title_sort spermatozoa morphometry and ultrastructure in estuarine crocodile (crocodylus porosus)
publisher Wolters Kluwer - Medknow Publications and Media
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/85882/
https://journals.lww.com/apjr/Fulltext/2020/09020/Spermatozoa_morphometry_and_ultrastructure_in.8.aspx#:~:text=Results%3A,(88.96%C2%B10.52)%20%CE%BCm.
_version_ 1781706652083290112
score 13.211869

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