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Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe

Ac DNA library was con structed inphagemid vector pT7T3 using messenger RNA (mRNA) extracted from IS - weekoil palm me socarp as template. The cDNA inserts were detected from agarose gel analysis and ranged in length from 130 to 1300 base pairs.This library was screened with a synthetic ACP pro...

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Main Author: Abd. Manaf, Mohamad Arif
Format: Thesis
Language:English
Published: 1994
Online Access:http://psasir.upm.edu.my/id/eprint/8588/1/FSAS_1994_6_A.pdf
http://psasir.upm.edu.my/id/eprint/8588/
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spelling my.upm.eprints.85882015-05-14T07:22:07Z http://psasir.upm.edu.my/id/eprint/8588/ Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe Abd. Manaf, Mohamad Arif Ac DNA library was con structed inphagemid vector pT7T3 using messenger RNA (mRNA) extracted from IS - weekoil palm me socarp as template. The cDNA inserts were detected from agarose gel analysis and ranged in length from 130 to 1300 base pairs.This library was screened with a synthetic ACP probe.Of the 2,000 clones screened using colony hybridisation analysis, 550 clones which gave high intensity of signal were then further screened using phagemid blot hybridisation analysis. This met hodyielded 184 clones which showed a high intensity of signal. The phagemids from the seclones were then screened by Southern hybridisation analysis using a synthetic ACP DNA probe. 1994 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8588/1/FSAS_1994_6_A.pdf Abd. Manaf, Mohamad Arif (1994) Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe. Masters thesis, Universiti Pertanian Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Ac DNA library was con structed inphagemid vector pT7T3 using messenger RNA (mRNA) extracted from IS - weekoil palm me socarp as template. The cDNA inserts were detected from agarose gel analysis and ranged in length from 130 to 1300 base pairs.This library was screened with a synthetic ACP probe.Of the 2,000 clones screened using colony hybridisation analysis, 550 clones which gave high intensity of signal were then further screened using phagemid blot hybridisation analysis. This met hodyielded 184 clones which showed a high intensity of signal. The phagemids from the seclones were then screened by Southern hybridisation analysis using a synthetic ACP DNA probe.
format Thesis
author Abd. Manaf, Mohamad Arif
spellingShingle Abd. Manaf, Mohamad Arif
Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
author_facet Abd. Manaf, Mohamad Arif
author_sort Abd. Manaf, Mohamad Arif
title Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_short Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_full Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_fullStr Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_full_unstemmed Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_sort screening for acyl carrier protein (acp) gene of oil palm (elaeis quineensia) using a synthetic acp dna probe
publishDate 1994
url http://psasir.upm.edu.my/id/eprint/8588/1/FSAS_1994_6_A.pdf
http://psasir.upm.edu.my/id/eprint/8588/
_version_ 1643824060810919936
score 13.211869

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