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The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture

Clinacanthus nutans also known as Sabah Snake Grass is a herb originated from Malaysia, Thailand and China belonging to the Acanthaceae family. This plant is a shrub with terete and glabrescent stems. It has a simple leaf arranged in an opposite manner with its shape lanceolate to ovate. This plant...

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Main Author: Zakaria, Nur Syazwani
Format: Project Paper Report
Language:English
Published: 2018
Online Access:http://psasir.upm.edu.my/id/eprint/85733/1/FP%202018%2011%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85733/
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spelling my.upm.eprints.857332021-06-16T04:42:43Z http://psasir.upm.edu.my/id/eprint/85733/ The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture Zakaria, Nur Syazwani Clinacanthus nutans also known as Sabah Snake Grass is a herb originated from Malaysia, Thailand and China belonging to the Acanthaceae family. This plant is a shrub with terete and glabrescent stems. It has a simple leaf arranged in an opposite manner with its shape lanceolate to ovate. This plant is traditionally used for disease treatment such as skin rashes, allergic and burns as well in curing snakebites. Recent research shows it is also efficiently used as remedies for herpes simplex and VZV lesions and alternative to cure cancer. The common method to propagate this plant is through stem cuttings, which requires much labour and time consuming and with limited number of planting materials produced. Therefore, tissue culture can be an alternative method to produce large number of offspring within a smaller space and short period of time. This experiment is carried out in In Vitro Laboratory, Department of Agriculture, UPM to determine the best concentration of kinetin that can maximize in vitro shoot proliferation from Clinacanthus nutans node culture. The experiment is carried out using Completely Randomised Design (CRD) with 7 treatments and 12 replications. Nodes (1cm) are excised from axenic cultures of Clinacanthus nutans and cultured at a slanting position in Murashige and Skoog (1962) medium with supplemented with different concentration of kinetin (0, 1.0, 2.0, 4.0, 6.0, 8.0 and 10.0mg/L). The culture is incubated for 8 weeks in the culture room. It is observed that all kinetin treatments including the control treatment proliferated buds within the range of 83-100%. However, elongation of explant are higher in 1.0mg/L and 2.0mg/L kinetin compared with explant supplemented with 8.0mg/L and 10.0mg/L kinetin. This showed that, higher concentration of kinetin can inhibit explant growth. 2018 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85733/1/FP%202018%2011%20IR.pdf Zakaria, Nur Syazwani (2018) The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Clinacanthus nutans also known as Sabah Snake Grass is a herb originated from Malaysia, Thailand and China belonging to the Acanthaceae family. This plant is a shrub with terete and glabrescent stems. It has a simple leaf arranged in an opposite manner with its shape lanceolate to ovate. This plant is traditionally used for disease treatment such as skin rashes, allergic and burns as well in curing snakebites. Recent research shows it is also efficiently used as remedies for herpes simplex and VZV lesions and alternative to cure cancer. The common method to propagate this plant is through stem cuttings, which requires much labour and time consuming and with limited number of planting materials produced. Therefore, tissue culture can be an alternative method to produce large number of offspring within a smaller space and short period of time. This experiment is carried out in In Vitro Laboratory, Department of Agriculture, UPM to determine the best concentration of kinetin that can maximize in vitro shoot proliferation from Clinacanthus nutans node culture. The experiment is carried out using Completely Randomised Design (CRD) with 7 treatments and 12 replications. Nodes (1cm) are excised from axenic cultures of Clinacanthus nutans and cultured at a slanting position in Murashige and Skoog (1962) medium with supplemented with different concentration of kinetin (0, 1.0, 2.0, 4.0, 6.0, 8.0 and 10.0mg/L). The culture is incubated for 8 weeks in the culture room. It is observed that all kinetin treatments including the control treatment proliferated buds within the range of 83-100%. However, elongation of explant are higher in 1.0mg/L and 2.0mg/L kinetin compared with explant supplemented with 8.0mg/L and 10.0mg/L kinetin. This showed that, higher concentration of kinetin can inhibit explant growth.
format Project Paper Report
author Zakaria, Nur Syazwani
spellingShingle Zakaria, Nur Syazwani
The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture
author_facet Zakaria, Nur Syazwani
author_sort Zakaria, Nur Syazwani
title The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture
title_short The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture
title_full The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture
title_fullStr The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture
title_full_unstemmed The effect of different kinetin concentration on shoot multiplication of Clinacanthus nutans node culture
title_sort effect of different kinetin concentration on shoot multiplication of clinacanthus nutans node culture
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/85733/1/FP%202018%2011%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85733/
_version_ 1703962178299625472
score 13.211869

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