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Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival

Gynura procumbens belongs to the Asteraceae family. This plant grows naturally in tropical Asia countries such as Malaysia and Indonesia. Its common name is longevity spinach or ‘sambung nyawa’ in Malay. It is a small herbaceous plant with ovate-elliptic leaves and is commonly used as a medical plan...

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Main Author: Aziz, Nurul Shaffiqqa
Format: Project Paper Report
Language:English
Published: 2018
Online Access:http://psasir.upm.edu.my/id/eprint/85718/1/FP%202018%208%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85718/
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spelling my.upm.eprints.857182021-06-16T04:34:15Z http://psasir.upm.edu.my/id/eprint/85718/ Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival Aziz, Nurul Shaffiqqa Gynura procumbens belongs to the Asteraceae family. This plant grows naturally in tropical Asia countries such as Malaysia and Indonesia. Its common name is longevity spinach or ‘sambung nyawa’ in Malay. It is a small herbaceous plant with ovate-elliptic leaves and is commonly used as a medical plant. Its leaves contain bioactive compound that can cure several diseases such as cancer, hypertension, diabetes mellitus, kidney discomfort, and inflammation. The common propagation method of this plant is through stem cutting but the number of cuttings that can be excised from a whole plant for propagation is very limited. Therefore, another alternative for mass propagation of Gynura procumbens plants is through tissue culture method. Currently there is no in vitro culture research carried out on Gynura procumbens. This initial study is aimed to reduce microbial contamination and to induce shoot generation from node cultures of Gynura procumbens. The experiment is carried out at In Vitro Laboratory, Department of Agriculture, Universiti Putra Malaysia (UPM) to determine the nodal segment which is less microbial contamination and to evaluate the best concentration and immersion time of explant in Clorox in reducing microbial contamination. The experiment is conducted using Completely Randomised Design (CRD). It was observed that no significant difference was observed on percentage of fungal and bacterial contamination, explants browning and explant survival by the nodes excised from different position on the mother stock plant. Fungal and bacterial contamination in these explants ranged between 16% to 73% and 6% to 20% respectively. The explant survival ranged between 80% and 90% in these explants indicating that these explants are good material for initiating culture establishment and later propagule multiplication. 2018 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85718/1/FP%202018%208%20IR.pdf Aziz, Nurul Shaffiqqa (2018) Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Gynura procumbens belongs to the Asteraceae family. This plant grows naturally in tropical Asia countries such as Malaysia and Indonesia. Its common name is longevity spinach or ‘sambung nyawa’ in Malay. It is a small herbaceous plant with ovate-elliptic leaves and is commonly used as a medical plant. Its leaves contain bioactive compound that can cure several diseases such as cancer, hypertension, diabetes mellitus, kidney discomfort, and inflammation. The common propagation method of this plant is through stem cutting but the number of cuttings that can be excised from a whole plant for propagation is very limited. Therefore, another alternative for mass propagation of Gynura procumbens plants is through tissue culture method. Currently there is no in vitro culture research carried out on Gynura procumbens. This initial study is aimed to reduce microbial contamination and to induce shoot generation from node cultures of Gynura procumbens. The experiment is carried out at In Vitro Laboratory, Department of Agriculture, Universiti Putra Malaysia (UPM) to determine the nodal segment which is less microbial contamination and to evaluate the best concentration and immersion time of explant in Clorox in reducing microbial contamination. The experiment is conducted using Completely Randomised Design (CRD). It was observed that no significant difference was observed on percentage of fungal and bacterial contamination, explants browning and explant survival by the nodes excised from different position on the mother stock plant. Fungal and bacterial contamination in these explants ranged between 16% to 73% and 6% to 20% respectively. The explant survival ranged between 80% and 90% in these explants indicating that these explants are good material for initiating culture establishment and later propagule multiplication.
format Project Paper Report
author Aziz, Nurul Shaffiqqa
spellingShingle Aziz, Nurul Shaffiqqa
Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival
author_facet Aziz, Nurul Shaffiqqa
author_sort Aziz, Nurul Shaffiqqa
title Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival
title_short Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival
title_full Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival
title_fullStr Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival
title_full_unstemmed Effect of nodes excised from different position of Gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival
title_sort effect of nodes excised from different position of gynura procumbens mother stock plant on reduction of microbial contamination and increasing in vitro explant survival
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/85718/1/FP%202018%208%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85718/
_version_ 1703962177870757888
score 13.211869

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