Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia

Fungi in the genus Fusarium are well known as soil-borne pathogen with worldwide distribution. Therefore, this study focused on isolation of Fusarium species from soil cultivated with watermelons, muskmelon, pumpkins, and cucumber in the east coast of Peninsular Malaysia by using dilution plate tech...

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Main Authors: Mohamed Sidique, Siti Nordahliawate, Mohd Zainuddin, Nur Ain Izzati, Azhari, Nur Azlin, Salleh, Baharuddin
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2012
Online Access:http://psasir.upm.edu.my/id/eprint/8558/1/Diversity%20of%20Fusarium%20species%20Isolated%20from%20Soil%20Cultivated%20with%20Cucurbits%20within%20East%20Coast%2C%20Peninsular%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/8558/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(2)%20May.%202012/20%20Pg%20381-386.pdf
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spelling my.upm.eprints.85582015-11-03T08:43:34Z http://psasir.upm.edu.my/id/eprint/8558/ Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia Mohamed Sidique, Siti Nordahliawate Mohd Zainuddin, Nur Ain Izzati Azhari, Nur Azlin Salleh, Baharuddin Fungi in the genus Fusarium are well known as soil-borne pathogen with worldwide distribution. Therefore, this study focused on isolation of Fusarium species from soil cultivated with watermelons, muskmelon, pumpkins, and cucumber in the east coast of Peninsular Malaysia by using dilution plate technique, direct plating and debris plating. The highest number of Fusarium species isolated was F. oxysporum with 687 (26.2%) colonies counted based on colony formation unit (CFU); the colonies of Fusarium/g soil = mean of Fusarium colonies x dilution factor/weight of dried soil (g). Other Fusarium species isolated were F. semitectum, F. solani, F. proliferatum, F. subglutinans and F. chlamydosporum. Throughout the studies, peptone pentachloronitrobenzene (PPA) medium, potato dextrose agar (PDA) and carnation leaf-piece agar (CLA) were regularly used to identify each Fusarium species by morphological means. Based on the Shannon-Weiner Index, Fusarium species diversity is much higher in Besut, Terengganu (H’=1.59). Fusarium species can be considered as a functionally important biological component of Fusarium fruit rot disease study in cucurbits. Universiti Putra Malaysia Press 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8558/1/Diversity%20of%20Fusarium%20species%20Isolated%20from%20Soil%20Cultivated%20with%20Cucurbits%20within%20East%20Coast%2C%20Peninsular%20Malaysia.pdf Mohamed Sidique, Siti Nordahliawate and Mohd Zainuddin, Nur Ain Izzati and Azhari, Nur Azlin and Salleh, Baharuddin (2012) Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia. Pertanika Journal of Tropical Agricultural Science, 35 (2). pp. 381-386. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(2)%20May.%202012/20%20Pg%20381-386.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Fungi in the genus Fusarium are well known as soil-borne pathogen with worldwide distribution. Therefore, this study focused on isolation of Fusarium species from soil cultivated with watermelons, muskmelon, pumpkins, and cucumber in the east coast of Peninsular Malaysia by using dilution plate technique, direct plating and debris plating. The highest number of Fusarium species isolated was F. oxysporum with 687 (26.2%) colonies counted based on colony formation unit (CFU); the colonies of Fusarium/g soil = mean of Fusarium colonies x dilution factor/weight of dried soil (g). Other Fusarium species isolated were F. semitectum, F. solani, F. proliferatum, F. subglutinans and F. chlamydosporum. Throughout the studies, peptone pentachloronitrobenzene (PPA) medium, potato dextrose agar (PDA) and carnation leaf-piece agar (CLA) were regularly used to identify each Fusarium species by morphological means. Based on the Shannon-Weiner Index, Fusarium species diversity is much higher in Besut, Terengganu (H’=1.59). Fusarium species can be considered as a functionally important biological component of Fusarium fruit rot disease study in cucurbits.
format Article
author Mohamed Sidique, Siti Nordahliawate
Mohd Zainuddin, Nur Ain Izzati
Azhari, Nur Azlin
Salleh, Baharuddin
spellingShingle Mohamed Sidique, Siti Nordahliawate
Mohd Zainuddin, Nur Ain Izzati
Azhari, Nur Azlin
Salleh, Baharuddin
Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia
author_facet Mohamed Sidique, Siti Nordahliawate
Mohd Zainuddin, Nur Ain Izzati
Azhari, Nur Azlin
Salleh, Baharuddin
author_sort Mohamed Sidique, Siti Nordahliawate
title Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia
title_short Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia
title_full Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia
title_fullStr Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia
title_full_unstemmed Diversity of Fusarium species isolated from soil cultivated with cucurbits within East Coast, Peninsular Malaysia
title_sort diversity of fusarium species isolated from soil cultivated with cucurbits within east coast, peninsular malaysia
publisher Universiti Putra Malaysia Press
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/8558/1/Diversity%20of%20Fusarium%20species%20Isolated%20from%20Soil%20Cultivated%20with%20Cucurbits%20within%20East%20Coast%2C%20Peninsular%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/8558/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(2)%20May.%202012/20%20Pg%20381-386.pdf
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score 13.211869