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Immobilization of anti-17 beta estradiol antibody on optical fiber surface

Antibody immobilization onto solid surface has been researched and studied exclusively for biosensor developments and immunoassay applications. In the project, two types of immobilization methods: physical and chemical adsorptions were carried out on optical fiber surface to study the immobilization...

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Main Author: Lee, May Ying
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/85117/1/FBSB%202015%20106%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85117/
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spelling my.upm.eprints.851172021-12-09T03:14:42Z http://psasir.upm.edu.my/id/eprint/85117/ Immobilization of anti-17 beta estradiol antibody on optical fiber surface Lee, May Ying Antibody immobilization onto solid surface has been researched and studied exclusively for biosensor developments and immunoassay applications. In the project, two types of immobilization methods: physical and chemical adsorptions were carried out on optical fiber surface to study the immobilization level measured by Optical Spectrum Analyzer (OSA). The chemical adsorption using the crosslinker, glutaraldehyde (GTA) which allows stronger strength of binding between anti-17 beta estradiol antibody and optical fiber surfaces, showed significantly high antibody immobilization level compared to physical adsorption. The weaker bond of attraction, van der Waals force is one of the limitations of the antibody immobilization by physical adsorption. The chemical reactions between the hydroxyl groups (-OH) on optical fiber surfaces and the silane chemical, 3-aminopropyl-triethoxysilane (APTES) before antibody immobilization were also studied. APTES was useful to modify the surface of optical fiber by chemical reaction to provide binding sites for GTA to activate the surface. Amino groups (-NH2) of antibody could eventually bind with the aldehyde groups (-CHO) of GTA to form amide bond which is a stronger linkage. In addition, the coating of GTA on optical fiber surface was also evaluated by trinocular microscope to investigate the differences between the bare optical fiber and GTA activated optical fiber. 2015 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85117/1/FBSB%202015%20106%20-%20IR.pdf Lee, May Ying (2015) Immobilization of anti-17 beta estradiol antibody on optical fiber surface. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Antibody immobilization onto solid surface has been researched and studied exclusively for biosensor developments and immunoassay applications. In the project, two types of immobilization methods: physical and chemical adsorptions were carried out on optical fiber surface to study the immobilization level measured by Optical Spectrum Analyzer (OSA). The chemical adsorption using the crosslinker, glutaraldehyde (GTA) which allows stronger strength of binding between anti-17 beta estradiol antibody and optical fiber surfaces, showed significantly high antibody immobilization level compared to physical adsorption. The weaker bond of attraction, van der Waals force is one of the limitations of the antibody immobilization by physical adsorption. The chemical reactions between the hydroxyl groups (-OH) on optical fiber surfaces and the silane chemical, 3-aminopropyl-triethoxysilane (APTES) before antibody immobilization were also studied. APTES was useful to modify the surface of optical fiber by chemical reaction to provide binding sites for GTA to activate the surface. Amino groups (-NH2) of antibody could eventually bind with the aldehyde groups (-CHO) of GTA to form amide bond which is a stronger linkage. In addition, the coating of GTA on optical fiber surface was also evaluated by trinocular microscope to investigate the differences between the bare optical fiber and GTA activated optical fiber.
format Project Paper Report
author Lee, May Ying
spellingShingle Lee, May Ying
Immobilization of anti-17 beta estradiol antibody on optical fiber surface
author_facet Lee, May Ying
author_sort Lee, May Ying
title Immobilization of anti-17 beta estradiol antibody on optical fiber surface
title_short Immobilization of anti-17 beta estradiol antibody on optical fiber surface
title_full Immobilization of anti-17 beta estradiol antibody on optical fiber surface
title_fullStr Immobilization of anti-17 beta estradiol antibody on optical fiber surface
title_full_unstemmed Immobilization of anti-17 beta estradiol antibody on optical fiber surface
title_sort immobilization of anti-17 beta estradiol antibody on optical fiber surface
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/85117/1/FBSB%202015%20106%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85117/
_version_ 1720438483404718080
score 13.211869

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