Isolation and characterization of Streptococcus from dental plaque samples
Dental caries is still a prevalent chronic condition that affecting health in the worldwide population. Other oral diseases, such as infection and tooth loss, are also very common. These diseases normally associated with discomfort and pain, eating and speech difficulties. Periodontal disease is...
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Main Author: | |
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Format: | Project Paper Report |
Language: | English |
Published: |
2015
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Online Access: | http://psasir.upm.edu.my/id/eprint/85108/1/FBSB%202015%2097%20-%20IR.pdf http://psasir.upm.edu.my/id/eprint/85108/ |
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Summary: | Dental caries is still a prevalent chronic condition that affecting health in the
worldwide population. Other oral diseases, such as infection and tooth loss, are also
very common. These diseases normally associated with discomfort and pain, eating
and speech difficulties. Periodontal disease is an inflammatory infection on the
gums, connective tissues and jaw bone that support the teeth. The root of these
complications is formation of dental plaque by specific group of bacteria. Prevention
practices, such as brushing teeth twice a day, using dental floss and antibacterial
rinse, eating a balanced diet are not practiced. Therefore, the most efficient method
to overcome this issue is eliminating the causative agents. This study is to isolate and
characterize dental plaque causing bacteria. The bacteria were isolated from dental
plaque sample. Preliminary identifications performed were macroscopic,
microscopic morphology and biochemical tests. The bacteria isolated were
characterized using Restriction Fragment Length Polymorphism (RFLP) and DNA
sequencing. Three isolates were selected and identified as Streptococcus mitis and
Streptococcus oralis. |
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