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The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.)

Thiamine is known to be important in human diet and its cofactor is required for the vital metabolic processes such as acetyl-CoA biosynthesis, amino acid biosynthesis, and Krebs and Calvin cycle. The active cofactor is thiamine pyrophosphate (TPP). There are findings about TPP which can act as a no...

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Main Author: Che Idris, Zainor Hafisah
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/85101/1/FBSB%202015%2090%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85101/
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spelling my.upm.eprints.851012021-12-09T03:21:19Z http://psasir.upm.edu.my/id/eprint/85101/ The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.) Che Idris, Zainor Hafisah Thiamine is known to be important in human diet and its cofactor is required for the vital metabolic processes such as acetyl-CoA biosynthesis, amino acid biosynthesis, and Krebs and Calvin cycle. The active cofactor is thiamine pyrophosphate (TPP). There are findings about TPP which can act as a non-cofactor function which is as stress-response molecule. In this study, the level of expression of THIC and THI1/THI4 gene transcripts upon induction of oxidative stress in oil palm was observed. The gene transcripts for the first two enzymes in the biosynthesis pathway, THIC and THI1/THI4 were successfully identified and amplified. Primers were designed based on the comparison of different plants such as Arabidopsis thaliana, Zea mays, Oryza sativa, and Alnus glutinosa. Also, the expression of these thiamine biosynthesis genes were investigated in response to the stress given to the oil palm. The gene transcripts’ expressions were analyzed via RT-PCR over a time point after the application of paraquat. As the oxidative stress induced the expression of the THIC gene transcript was increased by 20 % and the production of THI1/THI4 gene transcript was increased by 10 % upon the induction of oxidative stress. Thus, these findings support the suggestion that thiamine may play a role in plant protection against stress as it may lead to the overexpression of thiamine in general. 2015-06 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85101/1/FBSB%202015%2090%20-%20IR.pdf Che Idris, Zainor Hafisah (2015) The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.). [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Thiamine is known to be important in human diet and its cofactor is required for the vital metabolic processes such as acetyl-CoA biosynthesis, amino acid biosynthesis, and Krebs and Calvin cycle. The active cofactor is thiamine pyrophosphate (TPP). There are findings about TPP which can act as a non-cofactor function which is as stress-response molecule. In this study, the level of expression of THIC and THI1/THI4 gene transcripts upon induction of oxidative stress in oil palm was observed. The gene transcripts for the first two enzymes in the biosynthesis pathway, THIC and THI1/THI4 were successfully identified and amplified. Primers were designed based on the comparison of different plants such as Arabidopsis thaliana, Zea mays, Oryza sativa, and Alnus glutinosa. Also, the expression of these thiamine biosynthesis genes were investigated in response to the stress given to the oil palm. The gene transcripts’ expressions were analyzed via RT-PCR over a time point after the application of paraquat. As the oxidative stress induced the expression of the THIC gene transcript was increased by 20 % and the production of THI1/THI4 gene transcript was increased by 10 % upon the induction of oxidative stress. Thus, these findings support the suggestion that thiamine may play a role in plant protection against stress as it may lead to the overexpression of thiamine in general.
format Project Paper Report
author Che Idris, Zainor Hafisah
spellingShingle Che Idris, Zainor Hafisah
The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.)
author_facet Che Idris, Zainor Hafisah
author_sort Che Idris, Zainor Hafisah
title The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.)
title_short The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.)
title_full The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.)
title_fullStr The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.)
title_full_unstemmed The effect of oxidative stress towards the expressions of thiamine biosynthesis genes (THIC & THI1/THI4) in oil palm (Elaeis guineensis Jacq.)
title_sort effect of oxidative stress towards the expressions of thiamine biosynthesis genes (thic & thi1/thi4) in oil palm (elaeis guineensis jacq.)
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/85101/1/FBSB%202015%2090%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85101/
_version_ 1720438481085267968
score 13.211869

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