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Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil

Fusarium solani, F. oxysporum, Phytophthora palmivora and Pyricularia oryzae are best examples of plant pathogenic fungi that have the ability to cause enormous economic losses on crops worldwide annually. Formation of biofilm is one of the survival strategies for bacteria and fungi to adapt to thei...

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Main Author: Andrew, Bernice
Format: Project Paper Report
Language:English
Published: 2017
Online Access:http://psasir.upm.edu.my/id/eprint/85059/1/FP%202017%2026%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85059/
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spelling my.upm.eprints.850592021-10-25T07:13:06Z http://psasir.upm.edu.my/id/eprint/85059/ Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil Andrew, Bernice Fusarium solani, F. oxysporum, Phytophthora palmivora and Pyricularia oryzae are best examples of plant pathogenic fungi that have the ability to cause enormous economic losses on crops worldwide annually. Formation of biofilm is one of the survival strategies for bacteria and fungi to adapt to their surrounding environment, especially when exposed to the hostile environment. Thus, the objective of this study was to assess efficacy of tea tree oil to suppress formation of biofilm in fungi. Fusarium solani, F. oxysporum and P. oryzae were cultured in PDA medium while P. palmivora was cultured in CMA medium. Microtiter plate method and poisoned food technique were used in this study. It was revealed that tea tree oil (TTO) showed 100% mycelial inhibition on F. oxysporum and F. solani at concentration of 1000 ppm. TTO was found to be less effective against P. oryzae and P. palmivora. For 96-well plates, TTO was able to suppress the growth of biofilm at concentration of 50,000 ppm and 100,000 ppm. Biofilm quantification was determined based on the reduction of XTT (a tetrazolium salt) by metabolically active fungal biofilm cells. 2017 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85059/1/FP%202017%2026%20IR.pdf Andrew, Bernice (2017) Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Fusarium solani, F. oxysporum, Phytophthora palmivora and Pyricularia oryzae are best examples of plant pathogenic fungi that have the ability to cause enormous economic losses on crops worldwide annually. Formation of biofilm is one of the survival strategies for bacteria and fungi to adapt to their surrounding environment, especially when exposed to the hostile environment. Thus, the objective of this study was to assess efficacy of tea tree oil to suppress formation of biofilm in fungi. Fusarium solani, F. oxysporum and P. oryzae were cultured in PDA medium while P. palmivora was cultured in CMA medium. Microtiter plate method and poisoned food technique were used in this study. It was revealed that tea tree oil (TTO) showed 100% mycelial inhibition on F. oxysporum and F. solani at concentration of 1000 ppm. TTO was found to be less effective against P. oryzae and P. palmivora. For 96-well plates, TTO was able to suppress the growth of biofilm at concentration of 50,000 ppm and 100,000 ppm. Biofilm quantification was determined based on the reduction of XTT (a tetrazolium salt) by metabolically active fungal biofilm cells.
format Project Paper Report
author Andrew, Bernice
spellingShingle Andrew, Bernice
Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil
author_facet Andrew, Bernice
author_sort Andrew, Bernice
title Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil
title_short Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil
title_full Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil
title_fullStr Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil
title_full_unstemmed Suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil
title_sort suppression of biofilm formation on selected plant pathogenic fungi using tea tree oil
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/85059/1/FP%202017%2026%20IR.pdf
http://psasir.upm.edu.my/id/eprint/85059/
_version_ 1715191558307840000
score 13.211869

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