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Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety

Sperm morphology is the most reliable parameter for predicting fertility whereas sperm morphometry value as an indicator of reproductive capacity in males. Visual observation has led to widely varying results due to numerous factors such as the use of different staining procedures and lack of standa...

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Main Author: Abd Wahab, Suliza Abd Wahab
Format: Project Paper Report
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/83646/1/FPV%202016%2052%20IR.pdf
http://psasir.upm.edu.my/id/eprint/83646/
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spelling my.upm.eprints.836462020-10-08T07:33:33Z http://psasir.upm.edu.my/id/eprint/83646/ Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety Abd Wahab, Suliza Abd Wahab Sperm morphology is the most reliable parameter for predicting fertility whereas sperm morphometry value as an indicator of reproductive capacity in males. Visual observation has led to widely varying results due to numerous factors such as the use of different staining procedures and lack of standardization of staining techniques. Thus, this study was conducted to compare different cytology staining techniques and to identify the best staining methods for better semen evaluation. Seven semen samples were collected using a standardized electro ejaculator from Boer bucks at TPU UPM (n = 4), and Labu farms (n = 3). Each sample was primarily assessed using routine semen evaluation protocols and processed accordingly. The semen smears were stained using different stains namely Eosin-Nigrosin (EN), Giemsa (G), Diff-Quik (DQ) and Hematoxylin-Eosin (HE). The slides were examined and morphometric measurements of 50 randomly selected sperm in each stained slides were performed and compared. Morphometric analysis revealed the smallest values recorded in HE for measurements of the head. Results showed there are significant difference (p<0.05) of all the morphometric parameters between EN and HE and; DQ and HE. Higher values of head width and length recorded in all stains except HE. It was concluded that there were alteration of measurement in buck sperm morphology with different staining method used. 2016-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/83646/1/FPV%202016%2052%20IR.pdf Abd Wahab, Suliza Abd Wahab (2016) Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Sperm morphology is the most reliable parameter for predicting fertility whereas sperm morphometry value as an indicator of reproductive capacity in males. Visual observation has led to widely varying results due to numerous factors such as the use of different staining procedures and lack of standardization of staining techniques. Thus, this study was conducted to compare different cytology staining techniques and to identify the best staining methods for better semen evaluation. Seven semen samples were collected using a standardized electro ejaculator from Boer bucks at TPU UPM (n = 4), and Labu farms (n = 3). Each sample was primarily assessed using routine semen evaluation protocols and processed accordingly. The semen smears were stained using different stains namely Eosin-Nigrosin (EN), Giemsa (G), Diff-Quik (DQ) and Hematoxylin-Eosin (HE). The slides were examined and morphometric measurements of 50 randomly selected sperm in each stained slides were performed and compared. Morphometric analysis revealed the smallest values recorded in HE for measurements of the head. Results showed there are significant difference (p<0.05) of all the morphometric parameters between EN and HE and; DQ and HE. Higher values of head width and length recorded in all stains except HE. It was concluded that there were alteration of measurement in buck sperm morphology with different staining method used.
format Project Paper Report
author Abd Wahab, Suliza Abd Wahab
spellingShingle Abd Wahab, Suliza Abd Wahab
Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety
author_facet Abd Wahab, Suliza Abd Wahab
author_sort Abd Wahab, Suliza Abd Wahab
title Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety
title_short Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety
title_full Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety
title_fullStr Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety
title_full_unstemmed Comparison of different cytologic staining techniques on Boer goat spermatozoa morphology and morphomety
title_sort comparison of different cytologic staining techniques on boer goat spermatozoa morphology and morphomety
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/83646/1/FPV%202016%2052%20IR.pdf
http://psasir.upm.edu.my/id/eprint/83646/
_version_ 1680322396896100352
score 13.211869

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