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Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract

“Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed from solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echola...

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Main Author: Tawai, Darreen
Format: Project Paper Report
Language:English
Published: 2018
Online Access:http://psasir.upm.edu.my/id/eprint/83617/1/FPV%202018%2023%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/83617/
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spelling my.upm.eprints.836172020-10-06T02:02:50Z http://psasir.upm.edu.my/id/eprint/83617/ Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract Tawai, Darreen “Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed from solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolating swiftlets) and Collocalia (non-echolating swiftlets). The objective of this study is to determine the qualitative genes expression associated in lipid signalling pathways such as Acetyl-CoA acetyltransferase 2 (ACAT2), Diacylglycerol O-acyltransferase 2 (DGAT2), Low density lipoprotein receptor (LDLR), Sterol regulatory element binding transcription factor 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), Proprotein Convertase Subtilisin/kexin type 9 (PCSK9) in HepG2 mammalian cell with or without supplementation of EBN extract and exogenous lipid. Raw EBN were manually cleaned, dried in 60°C oven and left overnight. In this project, EBN supernatant were added with acetone using 1:2 ratio and kept in -80°C for an hour. Hep G2 cells were cultured in six wells plates and each wells were treated according to Base Control, Positive Control, Negative Control, Treatment 1, Treatment 2 and Treatment 3. In this study, there are significant changes in term of genes expression with supplementation of EBN extract especially in ACAT2 and LDLR genes. 2018-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/83617/1/FPV%202018%2023%20-%20IR.pdf Tawai, Darreen (2018) Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description “Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed from solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolating swiftlets) and Collocalia (non-echolating swiftlets). The objective of this study is to determine the qualitative genes expression associated in lipid signalling pathways such as Acetyl-CoA acetyltransferase 2 (ACAT2), Diacylglycerol O-acyltransferase 2 (DGAT2), Low density lipoprotein receptor (LDLR), Sterol regulatory element binding transcription factor 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), Proprotein Convertase Subtilisin/kexin type 9 (PCSK9) in HepG2 mammalian cell with or without supplementation of EBN extract and exogenous lipid. Raw EBN were manually cleaned, dried in 60°C oven and left overnight. In this project, EBN supernatant were added with acetone using 1:2 ratio and kept in -80°C for an hour. Hep G2 cells were cultured in six wells plates and each wells were treated according to Base Control, Positive Control, Negative Control, Treatment 1, Treatment 2 and Treatment 3. In this study, there are significant changes in term of genes expression with supplementation of EBN extract especially in ACAT2 and LDLR genes.
format Project Paper Report
author Tawai, Darreen
spellingShingle Tawai, Darreen
Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract
author_facet Tawai, Darreen
author_sort Tawai, Darreen
title Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract
title_short Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract
title_full Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract
title_fullStr Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract
title_full_unstemmed Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract
title_sort lipid signaling pathways genes expression level in hepg2 cells supplemented with exogenous lipid and edible bird nest (ebn) extract
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/83617/1/FPV%202018%2023%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/83617/
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score 13.211869

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