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Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA

Porcine Reproductive and Respiratory Syndrome (PRRS) is disease that is highly contagious and of great economic importance in Malaysia. Therefore, reliable and improved diagnostic methods are needed to help disease surveillance. This study evaluates the use of oral fluid samples instead of serum sam...

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Main Author: Kuiek, Ah Meng
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/83470/1/FPV%202015%2059%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/83470/
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spelling my.upm.eprints.834702020-09-28T08:20:53Z http://psasir.upm.edu.my/id/eprint/83470/ Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA Kuiek, Ah Meng Porcine Reproductive and Respiratory Syndrome (PRRS) is disease that is highly contagious and of great economic importance in Malaysia. Therefore, reliable and improved diagnostic methods are needed to help disease surveillance. This study evaluates the use of oral fluid samples instead of serum samples to detect PRRS by using IDEXX ELISA test kit. The study involves two pig farms located at Perak and Selangor, Malaysia. 35 animals were used as subjects from each farm. These 35 animals were divided into 7 different categories: gilts, young sows (2nd to 5th parities), old sows (6th parities and above), and weaners (10 weeks old, 15 weeks old, 20 weeks old, and 25 weeks old). Oral fluid and serum samples were collected from these animals individually whereas pen oral fluid samples were collected from weaners only. The oral fluid and serum samples were tested with IDEXX PRRS Oral Fluid Antibody Test Kit and IDEXX PRRS X3 Antibody Test Kit respectively. Statistical analysis shows that there is significant, strong, and positive correlation between samples for Farm A (p=0.0001, r=0.681) and significant, moderate, and positive correlation between samples for Farm B (p=0.0001, r=0.601). In conclusion, other than serum samples, oral fluids can also be used as diagnostic tool for PRRS surveillance in the farm. 2015-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/83470/1/FPV%202015%2059%20-%20IR.pdf Kuiek, Ah Meng (2015) Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Porcine Reproductive and Respiratory Syndrome (PRRS) is disease that is highly contagious and of great economic importance in Malaysia. Therefore, reliable and improved diagnostic methods are needed to help disease surveillance. This study evaluates the use of oral fluid samples instead of serum samples to detect PRRS by using IDEXX ELISA test kit. The study involves two pig farms located at Perak and Selangor, Malaysia. 35 animals were used as subjects from each farm. These 35 animals were divided into 7 different categories: gilts, young sows (2nd to 5th parities), old sows (6th parities and above), and weaners (10 weeks old, 15 weeks old, 20 weeks old, and 25 weeks old). Oral fluid and serum samples were collected from these animals individually whereas pen oral fluid samples were collected from weaners only. The oral fluid and serum samples were tested with IDEXX PRRS Oral Fluid Antibody Test Kit and IDEXX PRRS X3 Antibody Test Kit respectively. Statistical analysis shows that there is significant, strong, and positive correlation between samples for Farm A (p=0.0001, r=0.681) and significant, moderate, and positive correlation between samples for Farm B (p=0.0001, r=0.601). In conclusion, other than serum samples, oral fluids can also be used as diagnostic tool for PRRS surveillance in the farm.
format Project Paper Report
author Kuiek, Ah Meng
spellingShingle Kuiek, Ah Meng
Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA
author_facet Kuiek, Ah Meng
author_sort Kuiek, Ah Meng
title Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA
title_short Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA
title_full Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA
title_fullStr Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA
title_full_unstemmed Application of oral fluid to detect Porcine Reproductive and Respiratory Syndrome (PRRS) by using ELISA
title_sort application of oral fluid to detect porcine reproductive and respiratory syndrome (prrs) by using elisa
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/83470/1/FPV%202015%2059%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/83470/
_version_ 1680322382179336192
score 13.211869

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