Control of Vibrio alginolyticus in Artemia culture by treatment with bacterial probiotics
Probiotics are bacteria which can act as health promoter of other organism by modifying the ambient microbial community, enhancing feed nutritional value, enhancing the host response towards disease, or improving the quality of its ambient environment. Probiotics Micrococcus spp. (JAQ07) and Baci...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82878/1/FP%202013%20109%20-%20IR.pdf http://psasir.upm.edu.my/id/eprint/82878/ |
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| Summary: | Probiotics are bacteria which can act as health promoter of other organism by modifying
the ambient microbial community, enhancing feed nutritional value, enhancing the host
response towards disease, or improving the quality of its ambient environment.
Probiotics Micrococcus spp. (JAQ07) and Bacillus spp. (JAQ04) were used as potential
probiotics in this experiment. Both probiotics were identified as Gram-positive with
different morphology. Micrococcus spp. was a rod-shaped bacterium whereas Bacillus
spp. was cocci-shaped bacterium. In in-vivo assay, Artemia was used as a host and
treated with Bacillus spp. and Micrococcus spp. at different concentrations (102, 104 and
106 CFU ml-1) and then challenged with Vibrio alginolyticus at concentration 105 CFU
ml-1. Bacillus spp. able to enhance the survival of Artemia better compared with
Micrococcus spp. when challenged with Vibrio alginolyticus. Artemia treated with
Bacillus spp. at concentration of 106 CFU/ml and challenged with Vibrio alginolyticus
showed 70% of survival compared with the survival of challenged Artemia with Vibrio
alginolyticus only (20% survival rate) after 7 days. Meanwhile Artemia pre-incubation
with Micrococuss spp. and challenged with Vibrio alginolyticus showed 68% survival.
Both probiotics are not harmful because no significant of survival was found compared
to the control. Micrococcus spp. was able to slightly reduce the vibrios load in Artemia
and culture water. However, Bacillus spp. was not able to reduce the vibrios load in
water culture and Artemia. Nevertheless, both demonstrated good characteristics as
probiotic candidates for aquaculture. |
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