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Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells

This study investigated the effects of vitamins D and E on an insulin-resistant model and hypothesized that this treatment would reverse the effects of Alzheimer’s disease (AD) and improves insulin signalling. An insulin-resistant model was induced in SK-N-SH neuronal cells with a treatment of 250 n...

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Main Authors: Zaulkffali, Amirah Salwani, Md Razip, Nurliyana Najwa, Syed Alwi, Sharifah Sakinah, Abd Jalil, Afifah, Abd Mutalib, Mohd Sokhini, Gopalsamy, Banulata, Chang, Sui Kiat, Zainal, Zaida, Ibrahim, Nafissa Nadia, Zakaria, Zainul Amiruddin, Khaza'ai, Huzwah
Format: Article
Language:English
Published: MDPI 2019
Online Access:http://psasir.upm.edu.my/id/eprint/82729/1/Vitamin%20D%20.pdf
http://psasir.upm.edu.my/id/eprint/82729/
https://www.mdpi.com/2072-6643/11/10/2525
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spelling my.upm.eprints.827292021-06-02T09:13:09Z http://psasir.upm.edu.my/id/eprint/82729/ Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells Zaulkffali, Amirah Salwani Md Razip, Nurliyana Najwa Syed Alwi, Sharifah Sakinah Abd Jalil, Afifah Abd Mutalib, Mohd Sokhini Gopalsamy, Banulata Chang, Sui Kiat Zainal, Zaida Ibrahim, Nafissa Nadia Zakaria, Zainul Amiruddin Khaza'ai, Huzwah This study investigated the effects of vitamins D and E on an insulin-resistant model and hypothesized that this treatment would reverse the effects of Alzheimer’s disease (AD) and improves insulin signalling. An insulin-resistant model was induced in SK-N-SH neuronal cells with a treatment of 250 nM insulin and re-challenged with 100 nM at two different incubation time (16 h and 24 h). The effects of vitamin D (10 and 20 ng/mL), vitamin E in the form of tocotrienol-rich fraction (TRF) (200 ng/mL) and the combination of vitamins D and E on insulin signalling markers (IR, PI3K, GLUT3, GLUT4, and p-AKT), glucose uptake and AD markers (GSK3β and TAU) were determined using quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). The results demonstrated an improvement of the insulin signalling pathway upon treatment with vitamin D alone, with significant increases in IR, PI3K, GLUT3, GLUT4 expression levels, as well as AKT phosphorylation and glucose uptake, while GSK3β and TAU expression levels was decreased significantly. On the contrary, vitamin E alone, increased p-AKT, reduced the ROS as well as GSK3β and TAU but had no effect on the insulin signalling expression levels. The combination of vitamins D and E only showed significant increase in GLUT4, p-AKT, reduced ROS as well as GSK3β and TAU. Thus, the universal role of vitamin D, E alone and in combinations could be the potential nutritional agents in restoring the sensitivity of neuronal cells towards insulin and delaying the pathophysiological progression of AD. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82729/1/Vitamin%20D%20.pdf Zaulkffali, Amirah Salwani and Md Razip, Nurliyana Najwa and Syed Alwi, Sharifah Sakinah and Abd Jalil, Afifah and Abd Mutalib, Mohd Sokhini and Gopalsamy, Banulata and Chang, Sui Kiat and Zainal, Zaida and Ibrahim, Nafissa Nadia and Zakaria, Zainul Amiruddin and Khaza'ai, Huzwah (2019) Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells. Nutrients, 11 (10). pp. 1-20. ISSN 2072-6643 https://www.mdpi.com/2072-6643/11/10/2525 10.3390/nu11102525
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study investigated the effects of vitamins D and E on an insulin-resistant model and hypothesized that this treatment would reverse the effects of Alzheimer’s disease (AD) and improves insulin signalling. An insulin-resistant model was induced in SK-N-SH neuronal cells with a treatment of 250 nM insulin and re-challenged with 100 nM at two different incubation time (16 h and 24 h). The effects of vitamin D (10 and 20 ng/mL), vitamin E in the form of tocotrienol-rich fraction (TRF) (200 ng/mL) and the combination of vitamins D and E on insulin signalling markers (IR, PI3K, GLUT3, GLUT4, and p-AKT), glucose uptake and AD markers (GSK3β and TAU) were determined using quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). The results demonstrated an improvement of the insulin signalling pathway upon treatment with vitamin D alone, with significant increases in IR, PI3K, GLUT3, GLUT4 expression levels, as well as AKT phosphorylation and glucose uptake, while GSK3β and TAU expression levels was decreased significantly. On the contrary, vitamin E alone, increased p-AKT, reduced the ROS as well as GSK3β and TAU but had no effect on the insulin signalling expression levels. The combination of vitamins D and E only showed significant increase in GLUT4, p-AKT, reduced ROS as well as GSK3β and TAU. Thus, the universal role of vitamin D, E alone and in combinations could be the potential nutritional agents in restoring the sensitivity of neuronal cells towards insulin and delaying the pathophysiological progression of AD.
format Article
author Zaulkffali, Amirah Salwani
Md Razip, Nurliyana Najwa
Syed Alwi, Sharifah Sakinah
Abd Jalil, Afifah
Abd Mutalib, Mohd Sokhini
Gopalsamy, Banulata
Chang, Sui Kiat
Zainal, Zaida
Ibrahim, Nafissa Nadia
Zakaria, Zainul Amiruddin
Khaza'ai, Huzwah
spellingShingle Zaulkffali, Amirah Salwani
Md Razip, Nurliyana Najwa
Syed Alwi, Sharifah Sakinah
Abd Jalil, Afifah
Abd Mutalib, Mohd Sokhini
Gopalsamy, Banulata
Chang, Sui Kiat
Zainal, Zaida
Ibrahim, Nafissa Nadia
Zakaria, Zainul Amiruddin
Khaza'ai, Huzwah
Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells
author_facet Zaulkffali, Amirah Salwani
Md Razip, Nurliyana Najwa
Syed Alwi, Sharifah Sakinah
Abd Jalil, Afifah
Abd Mutalib, Mohd Sokhini
Gopalsamy, Banulata
Chang, Sui Kiat
Zainal, Zaida
Ibrahim, Nafissa Nadia
Zakaria, Zainul Amiruddin
Khaza'ai, Huzwah
author_sort Zaulkffali, Amirah Salwani
title Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells
title_short Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells
title_full Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells
title_fullStr Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells
title_full_unstemmed Vitamin D and Stimulates the PI3K-AKT signaling pathway in insulin resistant SK-N-SH neuronal cells
title_sort vitamin d and stimulates the pi3k-akt signaling pathway in insulin resistant sk-n-sh neuronal cells
publisher MDPI
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/82729/1/Vitamin%20D%20.pdf
http://psasir.upm.edu.my/id/eprint/82729/
https://www.mdpi.com/2072-6643/11/10/2525
_version_ 1702171457562345472
score 13.211869

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