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The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells

Commonly, stem cell culture is based on batch-type culture, which is laborious and expensive. We continuously cultured human pluripotent stem cells (hPSCs) on thermoresponsive dish surfaces, where hPSCs were partially detached on the same thermoresponsive dish by decreasing the temperature of the th...

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Main Authors: Sung, Tzu Cheng, Yang, Jia Sin, Yeh, Chih Chen, Liu, Ya Chu, Jiang, Yi Peng, Lu, Ming We, Ling, Qing Dong, Kumar, S. Suresh, Chang, Yung, Umezawa, Akihiro, Chen, Hao, Higuchi, Akon
Format: Article
Language:English
Published: Elsevier 2019
Online Access:http://psasir.upm.edu.my/id/eprint/82380/1/The%20design%20of%20a%20thermoresponsive.pdf
http://psasir.upm.edu.my/id/eprint/82380/
https://www.sciencedirect.com/science/article/abs/pii/S0142961219305101
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spelling my.upm.eprints.823802021-08-28T00:01:54Z http://psasir.upm.edu.my/id/eprint/82380/ The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells Sung, Tzu Cheng Yang, Jia Sin Yeh, Chih Chen Liu, Ya Chu Jiang, Yi Peng Lu, Ming We Ling, Qing Dong Kumar, S. Suresh Chang, Yung Umezawa, Akihiro Chen, Hao Higuchi, Akon Commonly, stem cell culture is based on batch-type culture, which is laborious and expensive. We continuously cultured human pluripotent stem cells (hPSCs) on thermoresponsive dish surfaces, where hPSCs were partially detached on the same thermoresponsive dish by decreasing the temperature of the thermoresponsive dish to be below the lower critical solution temperature for only 30 min. Then, the remaining cells were continuously cultured in fresh culture medium, and the detached stem cells were harvested in the exchanged culture medium. hPSCs were continuously cultured for ten cycles on the thermoresponsive dish surface, which was prepared by coating the surface with poly(N-isopropylacrylamide-co-styrene) and oligovitronectin-grafted poly(acrylic acid-co-styrene) or recombinant vitronectin for hPSC binding sites to maintain hPSC pluripotency. After ten cycles of continuous culture on the thermoresponsive dish surface, the detached cells expressed pluripotency proteins and had the ability to differentiate into cells derived from the three germ layers in vitro and in vivo. Furthermore, the detached cells differentiated into specific cell lineages, such as cardiomyocytes, with high efficiency. Elsevier 2019-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82380/1/The%20design%20of%20a%20thermoresponsive.pdf Sung, Tzu Cheng and Yang, Jia Sin and Yeh, Chih Chen and Liu, Ya Chu and Jiang, Yi Peng and Lu, Ming We and Ling, Qing Dong and Kumar, S. Suresh and Chang, Yung and Umezawa, Akihiro and Chen, Hao and Higuchi, Akon (2019) The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells. Biomaterials, 221. pp. 1-13. ISSN 0142-9612; ESSN: 1878-5905 https://www.sciencedirect.com/science/article/abs/pii/S0142961219305101 10.1016/j.biomaterials.2019.119411
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Commonly, stem cell culture is based on batch-type culture, which is laborious and expensive. We continuously cultured human pluripotent stem cells (hPSCs) on thermoresponsive dish surfaces, where hPSCs were partially detached on the same thermoresponsive dish by decreasing the temperature of the thermoresponsive dish to be below the lower critical solution temperature for only 30 min. Then, the remaining cells were continuously cultured in fresh culture medium, and the detached stem cells were harvested in the exchanged culture medium. hPSCs were continuously cultured for ten cycles on the thermoresponsive dish surface, which was prepared by coating the surface with poly(N-isopropylacrylamide-co-styrene) and oligovitronectin-grafted poly(acrylic acid-co-styrene) or recombinant vitronectin for hPSC binding sites to maintain hPSC pluripotency. After ten cycles of continuous culture on the thermoresponsive dish surface, the detached cells expressed pluripotency proteins and had the ability to differentiate into cells derived from the three germ layers in vitro and in vivo. Furthermore, the detached cells differentiated into specific cell lineages, such as cardiomyocytes, with high efficiency.
format Article
author Sung, Tzu Cheng
Yang, Jia Sin
Yeh, Chih Chen
Liu, Ya Chu
Jiang, Yi Peng
Lu, Ming We
Ling, Qing Dong
Kumar, S. Suresh
Chang, Yung
Umezawa, Akihiro
Chen, Hao
Higuchi, Akon
spellingShingle Sung, Tzu Cheng
Yang, Jia Sin
Yeh, Chih Chen
Liu, Ya Chu
Jiang, Yi Peng
Lu, Ming We
Ling, Qing Dong
Kumar, S. Suresh
Chang, Yung
Umezawa, Akihiro
Chen, Hao
Higuchi, Akon
The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells
author_facet Sung, Tzu Cheng
Yang, Jia Sin
Yeh, Chih Chen
Liu, Ya Chu
Jiang, Yi Peng
Lu, Ming We
Ling, Qing Dong
Kumar, S. Suresh
Chang, Yung
Umezawa, Akihiro
Chen, Hao
Higuchi, Akon
author_sort Sung, Tzu Cheng
title The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells
title_short The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells
title_full The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells
title_fullStr The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells
title_full_unstemmed The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells
title_sort design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells
publisher Elsevier
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/82380/1/The%20design%20of%20a%20thermoresponsive.pdf
http://psasir.upm.edu.my/id/eprint/82380/
https://www.sciencedirect.com/science/article/abs/pii/S0142961219305101
_version_ 1709669002808131584
score 13.211869

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