Staff View: Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’

Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’

To date, there is no standardized Fusarium bioassay protocol established owing partly to the wide variety of Fusarium oxysporum f. sp. cubense (Foc) isolates and banana cultivars present. Thus, validation of the infection parameters is deemed essential prior to each bioassay experiment. In the curre...

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Main Authors:	Mohd Yusuf, Yusmin, Khalid, Norzulaani, Al Obaidi, Jameel R., Baharum, Nadiya Akmal, Zaidi, Kamilatulhusna, Salleh, Baharuddin, Azuddin, Nurul Farizah, Abdul Aziz, Fashli Aziz, Munusamy, Umaiyal, Othman, Rofina Yasmin
Format:	Article
Language:	English
Published:	Universiti Putra Malaysia 2019
Online Access:	http://psasir.upm.edu.my/id/eprint/82245/1/Standardized%20bioassays%20an%20improved%20method%20for%20studying%20Fusarium%20oxysporum%20f.%20sp.%20cubense%20race%204%20%28FocR4%29%20pathogen%20stress%20response%20in%20Musa%20acuminata%20cv.%20%E2%80%98Berangan%E2%80%99.pdf http://psasir.upm.edu.my/id/eprint/82245/
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spelling	my.upm.eprints.822452020-12-16T05:54:04Z http://psasir.upm.edu.my/id/eprint/82245/ Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’ Mohd Yusuf, Yusmin Khalid, Norzulaani Al Obaidi, Jameel R. Baharum, Nadiya Akmal Zaidi, Kamilatulhusna Salleh, Baharuddin Azuddin, Nurul Farizah Abdul Aziz, Fashli Aziz Munusamy, Umaiyal Othman, Rofina Yasmin To date, there is no standardized Fusarium bioassay protocol established owing partly to the wide variety of Fusarium oxysporum f. sp. cubense (Foc) isolates and banana cultivars present. Thus, validation of the infection parameters is deemed essential prior to each bioassay experiment. In the current study, a simple standardized workflow was developed based on available assays for testing Fusarium wilt disease response in Musa acuminata using M. acuminata cv. ‘Berangan’ of tissue-culture origin as a model. The phenotypic assays were able to detect external disease symptoms less than one week post-inoculation, while the molecular approach using RT-qPCR identified differential expression of catalase (CAT), pathogenesis-related 10 (PR10), phenylalanine ammonia-lyase (PAL) and xylanase (XYL) genes as early as day 0. The transcript levels of PR10 and XYL fluctuated over 4 days of Foc Race 4 (FocR4 C1 HIR isolate) infection while the expression of CAT steadily increased over time. In contrast, PAL was highly upregulated at 2 days post-inoculation. These signature changes suggest that all genes tested might be involved in the early defense response of ‘Berangan’ plants against FocR4 infection. ‘Berangan’ cultivar was found to be highly susceptible to Foc Race 4 (C1 HIR isolate) with leaf symptoms index (LSI) and rhizome discoloration index (RDI) scores of 4.257 and 5.971, respectively. The procedure elaborated in this study can be used as a reference Foc bioassay for reproducible and comparable results possibly across cultivars and test isolates due to its simple steps aided by integration of phenotypic and molecular approach. Universiti Putra Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82245/1/Standardized%20bioassays%20an%20improved%20method%20for%20studying%20Fusarium%20oxysporum%20f.%20sp.%20cubense%20race%204%20%28FocR4%29%20pathogen%20stress%20response%20in%20Musa%20acuminata%20cv.%20%E2%80%98Berangan%E2%80%99.pdf Mohd Yusuf, Yusmin and Khalid, Norzulaani and Al Obaidi, Jameel R. and Baharum, Nadiya Akmal and Zaidi, Kamilatulhusna and Salleh, Baharuddin and Azuddin, Nurul Farizah and Abdul Aziz, Fashli Aziz and Munusamy, Umaiyal and Othman, Rofina Yasmin (2019) Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’. Asia-Pacific Journal of Molecular Biology and Biotechnology, 27 (3). pp. 101-112. ISSN 0128-7451 10.35118/apjmbb.2019.027.3.10
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	To date, there is no standardized Fusarium bioassay protocol established owing partly to the wide variety of Fusarium oxysporum f. sp. cubense (Foc) isolates and banana cultivars present. Thus, validation of the infection parameters is deemed essential prior to each bioassay experiment. In the current study, a simple standardized workflow was developed based on available assays for testing Fusarium wilt disease response in Musa acuminata using M. acuminata cv. ‘Berangan’ of tissue-culture origin as a model. The phenotypic assays were able to detect external disease symptoms less than one week post-inoculation, while the molecular approach using RT-qPCR identified differential expression of catalase (CAT), pathogenesis-related 10 (PR10), phenylalanine ammonia-lyase (PAL) and xylanase (XYL) genes as early as day 0. The transcript levels of PR10 and XYL fluctuated over 4 days of Foc Race 4 (FocR4 C1 HIR isolate) infection while the expression of CAT steadily increased over time. In contrast, PAL was highly upregulated at 2 days post-inoculation. These signature changes suggest that all genes tested might be involved in the early defense response of ‘Berangan’ plants against FocR4 infection. ‘Berangan’ cultivar was found to be highly susceptible to Foc Race 4 (C1 HIR isolate) with leaf symptoms index (LSI) and rhizome discoloration index (RDI) scores of 4.257 and 5.971, respectively. The procedure elaborated in this study can be used as a reference Foc bioassay for reproducible and comparable results possibly across cultivars and test isolates due to its simple steps aided by integration of phenotypic and molecular approach.
format	Article
author	Mohd Yusuf, Yusmin Khalid, Norzulaani Al Obaidi, Jameel R. Baharum, Nadiya Akmal Zaidi, Kamilatulhusna Salleh, Baharuddin Azuddin, Nurul Farizah Abdul Aziz, Fashli Aziz Munusamy, Umaiyal Othman, Rofina Yasmin
spellingShingle	Mohd Yusuf, Yusmin Khalid, Norzulaani Al Obaidi, Jameel R. Baharum, Nadiya Akmal Zaidi, Kamilatulhusna Salleh, Baharuddin Azuddin, Nurul Farizah Abdul Aziz, Fashli Aziz Munusamy, Umaiyal Othman, Rofina Yasmin Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’
author_facet	Mohd Yusuf, Yusmin Khalid, Norzulaani Al Obaidi, Jameel R. Baharum, Nadiya Akmal Zaidi, Kamilatulhusna Salleh, Baharuddin Azuddin, Nurul Farizah Abdul Aziz, Fashli Aziz Munusamy, Umaiyal Othman, Rofina Yasmin
author_sort	Mohd Yusuf, Yusmin
title	Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’
title_short	Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’
title_full	Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’
title_fullStr	Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’
title_full_unstemmed	Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’
title_sort	standardized bioassays: an improved method for studying fusarium oxysporum f. sp. cubense race 4 (focr4) pathogen stress response in musa acuminata cv. ‘berangan’
publisher	Universiti Putra Malaysia
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/82245/1/Standardized%20bioassays%20an%20improved%20method%20for%20studying%20Fusarium%20oxysporum%20f.%20sp.%20cubense%20race%204%20%28FocR4%29%20pathogen%20stress%20response%20in%20Musa%20acuminata%20cv.%20%E2%80%98Berangan%E2%80%99.pdf http://psasir.upm.edu.my/id/eprint/82245/
_version_	1687395161125945344
score	13.211869

Standardized bioassays: an improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’

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