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RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4

The expression profile of pathogenesis related genes are signatures of an infection response in plant cells. Pathogenic infections can increase or reduce gene expression in a plant system in a relatively specific pattern. These expression patterns can be used as standards in pathogenicity studies an...

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Main Authors: Munusamy, Umaiyal, Mohd Yusuf, Yusmin, Baharum, Nadiya Akmal, Zaidi, Kamilatulhusna, Othman, Rofina Yasmin
Format: Article
Language:English
Published: University of Agriculture 2019
Online Access:http://psasir.upm.edu.my/id/eprint/82053/1/RT-qPCR%20profiling%20.pdf
http://psasir.upm.edu.my/id/eprint/82053/
https://www.cabi.org/agbiotechnet/abstract/20193066569
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spelling my.upm.eprints.820532021-09-08T11:23:33Z http://psasir.upm.edu.my/id/eprint/82053/ RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4 Munusamy, Umaiyal Mohd Yusuf, Yusmin Baharum, Nadiya Akmal Zaidi, Kamilatulhusna Othman, Rofina Yasmin The expression profile of pathogenesis related genes are signatures of an infection response in plant cells. Pathogenic infections can increase or reduce gene expression in a plant system in a relatively specific pattern. These expression patterns can be used as standards in pathogenicity studies and, where phenotypic expression is normally used to gauge a plants response to infection, it could additionally present a more rapid and early screening reference tool. Three genes: catalase (CAT), pathogen related protein (PR10), and phenylalanine ammonia (PAL) all implicitly implicated in the plant disease response pathway were targeted for analysis during the infection of Fusarium oxysporum f. sp. cubense tropical race 4 (FOCR4) in banana Musa acuminata cv. Berangan seedlings after a standard challenge under growth room conditions. Distinct patterns of gene expression were observed at three infection time points by real time expression analysis. There was a sequential 10-fold reduction in expression for the PR gene while, the PAL and CAT genes were both upregulated. These results present a set of reference genes that could be used for screening of a plant’s response to Fusarium before the onset of symptoms. University of Agriculture 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82053/1/RT-qPCR%20profiling%20.pdf Munusamy, Umaiyal and Mohd Yusuf, Yusmin and Baharum, Nadiya Akmal and Zaidi, Kamilatulhusna and Othman, Rofina Yasmin (2019) RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4. Pakistan Journal of Agricultural Sciences, 56 (1). pp. 37-42. ISSN 0552-9034; ESSN: 2076-0906 https://www.cabi.org/agbiotechnet/abstract/20193066569 10.21162/PAKJAS/19.4296
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The expression profile of pathogenesis related genes are signatures of an infection response in plant cells. Pathogenic infections can increase or reduce gene expression in a plant system in a relatively specific pattern. These expression patterns can be used as standards in pathogenicity studies and, where phenotypic expression is normally used to gauge a plants response to infection, it could additionally present a more rapid and early screening reference tool. Three genes: catalase (CAT), pathogen related protein (PR10), and phenylalanine ammonia (PAL) all implicitly implicated in the plant disease response pathway were targeted for analysis during the infection of Fusarium oxysporum f. sp. cubense tropical race 4 (FOCR4) in banana Musa acuminata cv. Berangan seedlings after a standard challenge under growth room conditions. Distinct patterns of gene expression were observed at three infection time points by real time expression analysis. There was a sequential 10-fold reduction in expression for the PR gene while, the PAL and CAT genes were both upregulated. These results present a set of reference genes that could be used for screening of a plant’s response to Fusarium before the onset of symptoms.
format Article
author Munusamy, Umaiyal
Mohd Yusuf, Yusmin
Baharum, Nadiya Akmal
Zaidi, Kamilatulhusna
Othman, Rofina Yasmin
spellingShingle Munusamy, Umaiyal
Mohd Yusuf, Yusmin
Baharum, Nadiya Akmal
Zaidi, Kamilatulhusna
Othman, Rofina Yasmin
RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4
author_facet Munusamy, Umaiyal
Mohd Yusuf, Yusmin
Baharum, Nadiya Akmal
Zaidi, Kamilatulhusna
Othman, Rofina Yasmin
author_sort Munusamy, Umaiyal
title RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4
title_short RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4
title_full RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4
title_fullStr RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4
title_full_unstemmed RT-qPCR profiling of pathogenesis related genes in Musa acuminata cv. 'Berangan' seedlings challenged with Fusarium oxysporum F. SP. Cubense tropical race 4
title_sort rt-qpcr profiling of pathogenesis related genes in musa acuminata cv. 'berangan' seedlings challenged with fusarium oxysporum f. sp. cubense tropical race 4
publisher University of Agriculture
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/82053/1/RT-qPCR%20profiling%20.pdf
http://psasir.upm.edu.my/id/eprint/82053/
https://www.cabi.org/agbiotechnet/abstract/20193066569
_version_ 1710677154081013760
score 13.211869

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