Recombinant luteinizing hormone development to improve the reproductive performance of female Malaysia catfish, Hemibagrus nemurus (Valenciennes, 1840)
Gonadotropins (GTHs), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) are the major regulators of gonadal development in fish. This study reported the production of recombinant LH (rLH) in Hemibagrus nemurus. A single-chain rLH from H. nemurus was cloned into pET-32 and expressed in...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Central Fisheries Research Institute
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81945/1/Recombinant%20luteinizing%20hormone%20.pdf http://psasir.upm.edu.my/id/eprint/81945/ https://www.trjfas.org/abstract.php?id=1374 |
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| Summary: | Gonadotropins (GTHs), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) are the major regulators of gonadal development in fish. This study reported the production of recombinant LH (rLH) in Hemibagrus nemurus. A single-chain rLH from H. nemurus was cloned into pET-32 and expressed in Escherichia coli. A specific band at 45.4 kDa was detected; this band corresponded to the molecular size of fusion rLH. Immature female H. nemurus were divided into four different treatment groups. Each group received a single injection of either 1X PBS, 0.5 ml/kg Ovaprim, rLH50 μg/kg, or rLH150 μg/kg. Gonad histology revealed that the fish treated with rLH50 μg/kg, rLH150 μg/kg, and Ovaprim could induce oocyte maturation at 48h post injection (p.i.). The 17ᵝ-estradiol (E2) plasma level in fish treated with rLH50 μg/kg significantly increased after 12h p.i., and the level was sustained up to 24h p.i. The mRNA expression levels of GTH subunits also significantly increased in fish treated with rLH50 μg/kg after 48h p.i. Fish treated with Ovaprim showed significantly increased E2 plasma levels after 6h p.i., but this level decreased after 12h p.i. No significant increase was found among mRNA subunits in fish treated with Ovaprim after 48h p.i. Treatment with rLH150 μg/kg had a weak stimulatory effect on the E2 production and the mRNA transcript level of GTH subunits. |
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