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Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels

Biomaterials provide novel platforms to deliver stem cell and growth factor therapies for central nervous system (CNS) repair. The majority of these approaches have focused on the promotion of neural progenitor cells and neurogenesis. However, it is now increasingly recognized that glial responses a...

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Main Authors: Chan, Su Jing, Niu, Wanting, Hayakawa, Kazuhide, Hamanaka, Gen, Wang, Xiaoying, Cheah, Pike See, Guo, Shuzhen, Yu, Zhangyang, Arai, Ken, Selim, Magdy H., Kurisawa, Motoichi, Spector, Myron, Lo, Eng H.
Format: Article
Language:English
Published: Wiley Open Access 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81864/1/Promoting%20neuro-supportive.pdf
http://psasir.upm.edu.my/id/eprint/81864/
https://stemcellsjournals.onlinelibrary.wiley.com/doi/10.1002/sctm.19-0159
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spelling my.upm.eprints.818642021-09-08T04:42:55Z http://psasir.upm.edu.my/id/eprint/81864/ Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels Chan, Su Jing Niu, Wanting Hayakawa, Kazuhide Hamanaka, Gen Wang, Xiaoying Cheah, Pike See Guo, Shuzhen Yu, Zhangyang Arai, Ken Selim, Magdy H. Kurisawa, Motoichi Spector, Myron Lo, Eng H. Biomaterials provide novel platforms to deliver stem cell and growth factor therapies for central nervous system (CNS) repair. The majority of these approaches have focused on the promotion of neural progenitor cells and neurogenesis. However, it is now increasingly recognized that glial responses are critical for recovery in the entire neurovascular unit. In this study, we investigated the cellular effects of epidermal growth factor (EGF) containing hydrogels on primary astrocyte cultures. Both EGF alone and EGF‐hydrogel equally promoted astrocyte proliferation, but EGF‐hydrogels further enhanced astrocyte activation, as evidenced by a significantly elevated Glial fibrillary acidic protein (GFAP) gene expression. Thereafter, conditioned media from astrocytes activated by EGF‐hydrogel protected neurons against injury and promoted synaptic plasticity after oxygen–glucose deprivation. Taken together, these findings suggest that EGF‐hydrogels can shift astrocytes into neuro‐supportive phenotypes. Consistent with this idea, quantitative‐polymerase chain reaction (qPCR) demonstrated that EGF‐hydrogels shifted astrocytes in part by downregulating potentially negative A1‐like genes (Fbln5 and Rt1‐S3) and upregulating potentially beneficial A2‐like genes (Clcf1, Tgm1, and Ptgs2). Further studies are warranted to explore the idea of using biomaterials to modify astrocyte behavior and thus indirectly augment neuroprotection and neuroplasticity in the context of stem cell and growth factor therapies for the CNS. Stem Cells Translational Medicine 2019 Biomaterials provide novel platforms to deliver stem cell and growth factor therapies for central nervous system repair. Our data suggest that epidermal growth factor‐containing hydrogels can shift astrocytes into potentially beneficial A2‐like phenotypes that may augment neuroprotection and neuroplasticity during the recovery phase after brain injury. Wiley Open Access 2019-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81864/1/Promoting%20neuro-supportive.pdf Chan, Su Jing and Niu, Wanting and Hayakawa, Kazuhide and Hamanaka, Gen and Wang, Xiaoying and Cheah, Pike See and Guo, Shuzhen and Yu, Zhangyang and Arai, Ken and Selim, Magdy H. and Kurisawa, Motoichi and Spector, Myron and Lo, Eng H. (2019) Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels. Stem Cells Translational Medicine, 8 (12). pp. 1242-1248. ISSN 2157-6564; ESSN: 2157-6580 https://stemcellsjournals.onlinelibrary.wiley.com/doi/10.1002/sctm.19-0159 10.1002/sctm.19-0159
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Biomaterials provide novel platforms to deliver stem cell and growth factor therapies for central nervous system (CNS) repair. The majority of these approaches have focused on the promotion of neural progenitor cells and neurogenesis. However, it is now increasingly recognized that glial responses are critical for recovery in the entire neurovascular unit. In this study, we investigated the cellular effects of epidermal growth factor (EGF) containing hydrogels on primary astrocyte cultures. Both EGF alone and EGF‐hydrogel equally promoted astrocyte proliferation, but EGF‐hydrogels further enhanced astrocyte activation, as evidenced by a significantly elevated Glial fibrillary acidic protein (GFAP) gene expression. Thereafter, conditioned media from astrocytes activated by EGF‐hydrogel protected neurons against injury and promoted synaptic plasticity after oxygen–glucose deprivation. Taken together, these findings suggest that EGF‐hydrogels can shift astrocytes into neuro‐supportive phenotypes. Consistent with this idea, quantitative‐polymerase chain reaction (qPCR) demonstrated that EGF‐hydrogels shifted astrocytes in part by downregulating potentially negative A1‐like genes (Fbln5 and Rt1‐S3) and upregulating potentially beneficial A2‐like genes (Clcf1, Tgm1, and Ptgs2). Further studies are warranted to explore the idea of using biomaterials to modify astrocyte behavior and thus indirectly augment neuroprotection and neuroplasticity in the context of stem cell and growth factor therapies for the CNS. Stem Cells Translational Medicine 2019 Biomaterials provide novel platforms to deliver stem cell and growth factor therapies for central nervous system repair. Our data suggest that epidermal growth factor‐containing hydrogels can shift astrocytes into potentially beneficial A2‐like phenotypes that may augment neuroprotection and neuroplasticity during the recovery phase after brain injury.
format Article
author Chan, Su Jing
Niu, Wanting
Hayakawa, Kazuhide
Hamanaka, Gen
Wang, Xiaoying
Cheah, Pike See
Guo, Shuzhen
Yu, Zhangyang
Arai, Ken
Selim, Magdy H.
Kurisawa, Motoichi
Spector, Myron
Lo, Eng H.
spellingShingle Chan, Su Jing
Niu, Wanting
Hayakawa, Kazuhide
Hamanaka, Gen
Wang, Xiaoying
Cheah, Pike See
Guo, Shuzhen
Yu, Zhangyang
Arai, Ken
Selim, Magdy H.
Kurisawa, Motoichi
Spector, Myron
Lo, Eng H.
Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels
author_facet Chan, Su Jing
Niu, Wanting
Hayakawa, Kazuhide
Hamanaka, Gen
Wang, Xiaoying
Cheah, Pike See
Guo, Shuzhen
Yu, Zhangyang
Arai, Ken
Selim, Magdy H.
Kurisawa, Motoichi
Spector, Myron
Lo, Eng H.
author_sort Chan, Su Jing
title Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels
title_short Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels
title_full Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels
title_fullStr Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels
title_full_unstemmed Promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels
title_sort promoting neuro-supportive properties of astrocytes with epidermal growth factor hydrogels
publisher Wiley Open Access
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/81864/1/Promoting%20neuro-supportive.pdf
http://psasir.upm.edu.my/id/eprint/81864/
https://stemcellsjournals.onlinelibrary.wiley.com/doi/10.1002/sctm.19-0159
_version_ 1710677148869591040
score 13.211869

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