Staff View: Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism

Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism

This paper describes the development of a lab-on-chip (LOC) device that can perform reliable online detection in continuous-flow systems for microorganisms. The objective of this work was to examine the performance of a fibre optic detection system integrated into a LOC device. The microfluidic sys...

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Main Authors:	Kamuri, Mohd Firdaus, Zainal Abidin, Zurina, Lee, Hao Jun, Yaacob, Mohd Hanif, Hamidon, Mohd Nizar, Md Yunus, Nurul Amziah, Kamarudin, Suryani
Format:	Article
Language:	English
Published:	Hindawi 2019
Online Access:	http://psasir.upm.edu.my/id/eprint/81669/1/FIBRE.pdf http://psasir.upm.edu.my/id/eprint/81669/ https://www.hindawi.com/journals/js/2019/1026905/
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spelling	my.upm.eprints.816692021-04-29T01:32:08Z http://psasir.upm.edu.my/id/eprint/81669/ Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism Kamuri, Mohd Firdaus Zainal Abidin, Zurina Lee, Hao Jun Yaacob, Mohd Hanif Hamidon, Mohd Nizar Md Yunus, Nurul Amziah Kamarudin, Suryani This paper describes the development of a lab-on-chip (LOC) device that can perform reliable online detection in continuous-flow systems for microorganisms. The objective of this work was to examine the performance of a fibre optic detection system integrated into a LOC device. The microfluidic system was fabricated using dry film resist (DFR), integrated with multimode fibre pigtails in the LOC. Subsequently, the performance of the fibre optic detection was evaluated by its absorbance spectra, detection limit, repeatability and reproducibility, and response time. The analysis was carried out using a constant flow rate for three different types of microorganisms which are Escherichia coli, Saccharomyces cerevisiae, and Aeromonas hydrophila. Under the experimental conditions used in this study, the detection limit of 1 0 × 105 cells/mL for both A. hydrophila and E. coli, while a detection limit of 1 0 × 106 cells/mL for S. cerevisiae cells were measured. The results also revealed that the device showed good repeatability with standard deviations less than 0.2 for A. hydrophila and E. coli, while standard deviations for S. cerevisiae were larger than 1.0. The response times for A. hydrophila, E. coli, and S. cerevisiae were 104 s, 122 s, and 78 s, respectively, although significant errors were recorded for all three species for reproducibility experiment. It was found that the device showed generally good sensitivity, with the highest sensitivity towards S. cerevisiae. These findings suggest that an integrated LOC device, with embedded multimode fibre pigtails, can be a reliable instrument for microorganism detection. Hindawi 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81669/1/FIBRE.pdf Kamuri, Mohd Firdaus and Zainal Abidin, Zurina and Lee, Hao Jun and Yaacob, Mohd Hanif and Hamidon, Mohd Nizar and Md Yunus, Nurul Amziah and Kamarudin, Suryani (2019) Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism. Journal of Sensors, 2019. art. no. 1026905. pp. 1-10. ISSN 1687-725X; ESSN: 1687-7268 https://www.hindawi.com/journals/js/2019/1026905/ 10.1155/2019/1026905
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	This paper describes the development of a lab-on-chip (LOC) device that can perform reliable online detection in continuous-flow systems for microorganisms. The objective of this work was to examine the performance of a fibre optic detection system integrated into a LOC device. The microfluidic system was fabricated using dry film resist (DFR), integrated with multimode fibre pigtails in the LOC. Subsequently, the performance of the fibre optic detection was evaluated by its absorbance spectra, detection limit, repeatability and reproducibility, and response time. The analysis was carried out using a constant flow rate for three different types of microorganisms which are Escherichia coli, Saccharomyces cerevisiae, and Aeromonas hydrophila. Under the experimental conditions used in this study, the detection limit of 1 0 × 105 cells/mL for both A. hydrophila and E. coli, while a detection limit of 1 0 × 106 cells/mL for S. cerevisiae cells were measured. The results also revealed that the device showed good repeatability with standard deviations less than 0.2 for A. hydrophila and E. coli, while standard deviations for S. cerevisiae were larger than 1.0. The response times for A. hydrophila, E. coli, and S. cerevisiae were 104 s, 122 s, and 78 s, respectively, although significant errors were recorded for all three species for reproducibility experiment. It was found that the device showed generally good sensitivity, with the highest sensitivity towards S. cerevisiae. These findings suggest that an integrated LOC device, with embedded multimode fibre pigtails, can be a reliable instrument for microorganism detection.
format	Article
author	Kamuri, Mohd Firdaus Zainal Abidin, Zurina Lee, Hao Jun Yaacob, Mohd Hanif Hamidon, Mohd Nizar Md Yunus, Nurul Amziah Kamarudin, Suryani
spellingShingle	Kamuri, Mohd Firdaus Zainal Abidin, Zurina Lee, Hao Jun Yaacob, Mohd Hanif Hamidon, Mohd Nizar Md Yunus, Nurul Amziah Kamarudin, Suryani Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism
author_facet	Kamuri, Mohd Firdaus Zainal Abidin, Zurina Lee, Hao Jun Yaacob, Mohd Hanif Hamidon, Mohd Nizar Md Yunus, Nurul Amziah Kamarudin, Suryani
author_sort	Kamuri, Mohd Firdaus
title	Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism
title_short	Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism
title_full	Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism
title_fullStr	Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism
title_full_unstemmed	Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism
title_sort	performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism
publisher	Hindawi
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/81669/1/FIBRE.pdf http://psasir.upm.edu.my/id/eprint/81669/ https://www.hindawi.com/journals/js/2019/1026905/
_version_	1698698931914932224
score	13.211869

Performance evaluation of free-space fibre optic detection in a lab-on-chip for microorganism

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