One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia
Background: In 2015, Singapore had the first and only reported foodborne outbreak of invasive disease caused by the group B Streptococcus (GBS; Streptococcus agalactiae). Disease, predominantly septic arthritis and meningitis, was associated with sequence type (ST)283, acquired from eating raw farme...
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2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81541/1/One%20hypervirulent%20clone%2C%20sequence%20type%20283%2C%20accounts%20for%20a%20large%20proportion%20of%20invasive%20Streptococcus%20agalactiae%20isolated%20from%20humans%20and%20diseased%20tilapia%20in%20Southeast%20Asia.pdf http://psasir.upm.edu.my/id/eprint/81541/ https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007421 |
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Background: In 2015, Singapore had the first and only reported foodborne outbreak of invasive disease caused by the group B Streptococcus (GBS; Streptococcus agalactiae). Disease, predominantly septic arthritis and meningitis, was associated with sequence type (ST)283, acquired from eating raw farmed freshwater fish. Although GBS sepsis is well-described in neonates and older adults with co-morbidities, this outbreak affected non-pregnant and younger adults with fewer co-morbidities, suggesting greater virulence. Before 2015 ST283 had only been reported from twenty humans in Hong Kong and two in France, and from one fish in Thailand. We hypothesised that ST283 was causing region-wide infection in Southeast Asia. Methodology/Principal findings: We performed a literature review, whole genome sequencing on 145 GBS isolates collected from six Southeast Asian countries, and phylogenetic analysis on 7,468 GBS sequences including 227 variants of ST283 from humans and animals. Although almost absent outside Asia, ST283 was found in all invasive Asian collections analysed, from 1995 to 2017. It accounted for 29/38 (76%) human isolates in Lao PDR, 102/139 (73%) in Thailand, 4/13 (31%) in Vietnam, and 167/739 (23%) in Singapore. ST283 and its variants were found in 62/62 (100%) tilapia from 14 outbreak sites in Malaysia and Vietnam, in seven fish species in Singapore markets, and a diseased frog in China. Conclusions: GBS ST283 is widespread in Southeast Asia, where it accounts for a large proportion of bacteraemic GBS, and causes disease and economic loss in aquaculture. If human ST283 is fishborne, as in the Singapore outbreak, then GBS sepsis in Thailand and Lao PDR is predominantly a foodborne disease. However, whether transmission is from aquaculture to humans, or vice versa, or involves an unidentified reservoir remains unknown. Creation of cross-border collaborations in human and animal health are needed to complete the epidemiological picture. |
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Barkham, Timothy Zadoks, Ruth N. Azmai, Mohammad Noor Amal Baker, Stephen Bich, Vu Thi Ngoc Chalker, Victoria Chau, Man Ling Dance, David Deepak, Rama Narayana Doorn, H. Rogier Van Gutierrez, Ramona A. Holmes, Mark A. Huong, Lan Nguyen Phu Koh, Tse Hsien Martins, Elisabete Mehershahi, Kurosh Newton, Paul Ng, Lee Ching Phuoc, Nguyen Ngoc Sangwichian, Ornuma Sawatwong, Pongpun Surin, Uraiwan Tan, Thean Yen Tang, Wen Ying Thuy, Nguyen Vu Turner, Paul Vongsouvath, Manivanh Zhang, Defeng WhistlerI, Toni Chen, Swaine L. |
| spellingShingle |
Barkham, Timothy Zadoks, Ruth N. Azmai, Mohammad Noor Amal Baker, Stephen Bich, Vu Thi Ngoc Chalker, Victoria Chau, Man Ling Dance, David Deepak, Rama Narayana Doorn, H. Rogier Van Gutierrez, Ramona A. Holmes, Mark A. Huong, Lan Nguyen Phu Koh, Tse Hsien Martins, Elisabete Mehershahi, Kurosh Newton, Paul Ng, Lee Ching Phuoc, Nguyen Ngoc Sangwichian, Ornuma Sawatwong, Pongpun Surin, Uraiwan Tan, Thean Yen Tang, Wen Ying Thuy, Nguyen Vu Turner, Paul Vongsouvath, Manivanh Zhang, Defeng WhistlerI, Toni Chen, Swaine L. One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia |
| author_facet |
Barkham, Timothy Zadoks, Ruth N. Azmai, Mohammad Noor Amal Baker, Stephen Bich, Vu Thi Ngoc Chalker, Victoria Chau, Man Ling Dance, David Deepak, Rama Narayana Doorn, H. Rogier Van Gutierrez, Ramona A. Holmes, Mark A. Huong, Lan Nguyen Phu Koh, Tse Hsien Martins, Elisabete Mehershahi, Kurosh Newton, Paul Ng, Lee Ching Phuoc, Nguyen Ngoc Sangwichian, Ornuma Sawatwong, Pongpun Surin, Uraiwan Tan, Thean Yen Tang, Wen Ying Thuy, Nguyen Vu Turner, Paul Vongsouvath, Manivanh Zhang, Defeng WhistlerI, Toni Chen, Swaine L. |
| author_sort |
Barkham, Timothy |
| title |
One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia |
| title_short |
One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia |
| title_full |
One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia |
| title_fullStr |
One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia |
| title_full_unstemmed |
One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia |
| title_sort |
one hypervirulent clone, sequence type 283, accounts for a large proportion of invasive streptococcus agalactiae isolated from humans and diseased tilapia in southeast asia |
| publisher |
Public Library of Science |
| publishDate |
2019 |
| url |
http://psasir.upm.edu.my/id/eprint/81541/1/One%20hypervirulent%20clone%2C%20sequence%20type%20283%2C%20accounts%20for%20a%20large%20proportion%20of%20invasive%20Streptococcus%20agalactiae%20isolated%20from%20humans%20and%20diseased%20tilapia%20in%20Southeast%20Asia.pdf http://psasir.upm.edu.my/id/eprint/81541/ https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007421 |
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my.upm.eprints.815412020-10-28T19:24:06Z http://psasir.upm.edu.my/id/eprint/81541/ One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia Barkham, Timothy Zadoks, Ruth N. Azmai, Mohammad Noor Amal Baker, Stephen Bich, Vu Thi Ngoc Chalker, Victoria Chau, Man Ling Dance, David Deepak, Rama Narayana Doorn, H. Rogier Van Gutierrez, Ramona A. Holmes, Mark A. Huong, Lan Nguyen Phu Koh, Tse Hsien Martins, Elisabete Mehershahi, Kurosh Newton, Paul Ng, Lee Ching Phuoc, Nguyen Ngoc Sangwichian, Ornuma Sawatwong, Pongpun Surin, Uraiwan Tan, Thean Yen Tang, Wen Ying Thuy, Nguyen Vu Turner, Paul Vongsouvath, Manivanh Zhang, Defeng WhistlerI, Toni Chen, Swaine L. Background: In 2015, Singapore had the first and only reported foodborne outbreak of invasive disease caused by the group B Streptococcus (GBS; Streptococcus agalactiae). Disease, predominantly septic arthritis and meningitis, was associated with sequence type (ST)283, acquired from eating raw farmed freshwater fish. Although GBS sepsis is well-described in neonates and older adults with co-morbidities, this outbreak affected non-pregnant and younger adults with fewer co-morbidities, suggesting greater virulence. Before 2015 ST283 had only been reported from twenty humans in Hong Kong and two in France, and from one fish in Thailand. We hypothesised that ST283 was causing region-wide infection in Southeast Asia. Methodology/Principal findings: We performed a literature review, whole genome sequencing on 145 GBS isolates collected from six Southeast Asian countries, and phylogenetic analysis on 7,468 GBS sequences including 227 variants of ST283 from humans and animals. Although almost absent outside Asia, ST283 was found in all invasive Asian collections analysed, from 1995 to 2017. It accounted for 29/38 (76%) human isolates in Lao PDR, 102/139 (73%) in Thailand, 4/13 (31%) in Vietnam, and 167/739 (23%) in Singapore. ST283 and its variants were found in 62/62 (100%) tilapia from 14 outbreak sites in Malaysia and Vietnam, in seven fish species in Singapore markets, and a diseased frog in China. Conclusions: GBS ST283 is widespread in Southeast Asia, where it accounts for a large proportion of bacteraemic GBS, and causes disease and economic loss in aquaculture. If human ST283 is fishborne, as in the Singapore outbreak, then GBS sepsis in Thailand and Lao PDR is predominantly a foodborne disease. However, whether transmission is from aquaculture to humans, or vice versa, or involves an unidentified reservoir remains unknown. Creation of cross-border collaborations in human and animal health are needed to complete the epidemiological picture. Public Library of Science 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81541/1/One%20hypervirulent%20clone%2C%20sequence%20type%20283%2C%20accounts%20for%20a%20large%20proportion%20of%20invasive%20Streptococcus%20agalactiae%20isolated%20from%20humans%20and%20diseased%20tilapia%20in%20Southeast%20Asia.pdf Barkham, Timothy and Zadoks, Ruth N. and Azmai, Mohammad Noor Amal and Baker, Stephen and Bich, Vu Thi Ngoc and Chalker, Victoria and Chau, Man Ling and Dance, David and Deepak, Rama Narayana and Doorn, H. Rogier Van and Gutierrez, Ramona A. and Holmes, Mark A. and Huong, Lan Nguyen Phu and Koh, Tse Hsien and Martins, Elisabete and Mehershahi, Kurosh and Newton, Paul and Ng, Lee Ching and Phuoc, Nguyen Ngoc and Sangwichian, Ornuma and Sawatwong, Pongpun and Surin, Uraiwan and Tan, Thean Yen and Tang, Wen Ying and Thuy, Nguyen Vu and Turner, Paul and Vongsouvath, Manivanh and Zhang, Defeng and WhistlerI, Toni and Chen, Swaine L. (2019) One hypervirulent clone, sequence type 283, accounts for a large proportion of invasive Streptococcus agalactiae isolated from humans and diseased tilapia in Southeast Asia. PLoS Neglected Tropical Diseases, 13 (6). art. no. e0007421. pp. 1-20. ISSN 1935-2727; ESSN: 1935-2735 https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007421 10.1371/journal.pntd.0007421 |
| score |
13.211869 |