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Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel

Mangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vit...

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Main Authors: Lasano, Nur Fatimah, Haji Hamid, Azizah, Karim, Roselina, Pak Dek, Mohd Sabri, Shukri, Radhiah, Ramli, Nurul Shazini
Format: Article
Published: Multidisciplinary Digital Publishing Institute 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81512/
https://www.mdpi.com/1420-3049/24/2/320
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spelling my.upm.eprints.815122024-05-06T07:45:19Z http://psasir.upm.edu.my/id/eprint/81512/ Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel Lasano, Nur Fatimah Haji Hamid, Azizah Karim, Roselina Pak Dek, Mohd Sabri Shukri, Radhiah Ramli, Nurul Shazini Mangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vitamins and minerals analyses), the anti-diabetic activities and phytochemical profile of M. odorata peel and seed kernel were investigated for the first time. The results indicated that seed kernel rich in fat, protein, carbohydrate, and ash while peel contained significantly greater amount of fiber, minerals, β-Carotene and ascorbic acid compared to seed kernel. The samples were then extracted using different solvents (acetone, ethanol, methanol at 60%, v/v and pure deionized water) and their anti-diabetic activities (α-amylase and α-glucosidase inhibition assay) were determined. Seed kernel had the lowest IC50 values for α-amylase and α-glucosidase inhibition assay in 60% ethanol and 60% acetone, respectively. Due to the toxic effect and high volatility of acetone, the ethanolic extracts of samples were further analyses for their phytochemical profile using high performance liquid chromatography-mass spectrometry (LC-MS) and ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS). The most abundant compounds identified were phenolic acid, ellagic acid, and flavonoid. These findings suggest that M. odorata fruit wastes, especially the seed kernel possesses promising ability to be used as functional ingredient in the food industry. Multidisciplinary Digital Publishing Institute 2019 Article PeerReviewed Lasano, Nur Fatimah and Haji Hamid, Azizah and Karim, Roselina and Pak Dek, Mohd Sabri and Shukri, Radhiah and Ramli, Nurul Shazini (2019) Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel. Molecules, 24 (2). art. no. 320. pp. 1-20. ISSN 1420-3049 https://www.mdpi.com/1420-3049/24/2/320 10.3390/molecules24020320
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Mangifera odorata fruit, the hybrid forms between M. indica (mango) and M. foetida (bacang), has been shown to exhibit potential antioxidant activity, and the fruit waste could demonstrate functional and nutritional potential. In the present study, the nutritional composition (proximate, sugars, vitamins and minerals analyses), the anti-diabetic activities and phytochemical profile of M. odorata peel and seed kernel were investigated for the first time. The results indicated that seed kernel rich in fat, protein, carbohydrate, and ash while peel contained significantly greater amount of fiber, minerals, β-Carotene and ascorbic acid compared to seed kernel. The samples were then extracted using different solvents (acetone, ethanol, methanol at 60%, v/v and pure deionized water) and their anti-diabetic activities (α-amylase and α-glucosidase inhibition assay) were determined. Seed kernel had the lowest IC50 values for α-amylase and α-glucosidase inhibition assay in 60% ethanol and 60% acetone, respectively. Due to the toxic effect and high volatility of acetone, the ethanolic extracts of samples were further analyses for their phytochemical profile using high performance liquid chromatography-mass spectrometry (LC-MS) and ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS). The most abundant compounds identified were phenolic acid, ellagic acid, and flavonoid. These findings suggest that M. odorata fruit wastes, especially the seed kernel possesses promising ability to be used as functional ingredient in the food industry.
format Article
author Lasano, Nur Fatimah
Haji Hamid, Azizah
Karim, Roselina
Pak Dek, Mohd Sabri
Shukri, Radhiah
Ramli, Nurul Shazini
spellingShingle Lasano, Nur Fatimah
Haji Hamid, Azizah
Karim, Roselina
Pak Dek, Mohd Sabri
Shukri, Radhiah
Ramli, Nurul Shazini
Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel
author_facet Lasano, Nur Fatimah
Haji Hamid, Azizah
Karim, Roselina
Pak Dek, Mohd Sabri
Shukri, Radhiah
Ramli, Nurul Shazini
author_sort Lasano, Nur Fatimah
title Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel
title_short Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel
title_full Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel
title_fullStr Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel
title_full_unstemmed Nutritional composition, anti-diabetic properties and identification of active compounds using UHPLC-ESI-Orbitrap-MS/MS in Mangifera odorata L. peel and seed kernel
title_sort nutritional composition, anti-diabetic properties and identification of active compounds using uhplc-esi-orbitrap-ms/ms in mangifera odorata l. peel and seed kernel
publisher Multidisciplinary Digital Publishing Institute
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/81512/
https://www.mdpi.com/1420-3049/24/2/320
_version_ 1800093757155049472
score 13.211869

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