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Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens

Fowl adenovirus (FAdV) is the causative agent of inclusion body hepatitis (IBH) in chickens with significant economic losses due to high mortality and poor production. It was objectives of the study to attenuate and determine the molecular characteristic of FAdV isolate (UPM1137) of Malaysia passage...

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Main Authors: Mohamed Sohaimi, Norfitriah, Bejo, Mohd Hair, Omar, Abdul Rahman, Ideris, Aini, Mat Isa, Nurulfiza
Format: Article
Language:English
Published: Public Library of Science 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81403/1/FOWL.pdf
http://psasir.upm.edu.my/id/eprint/81403/
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225863
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spelling my.upm.eprints.814032021-06-13T23:08:54Z http://psasir.upm.edu.my/id/eprint/81403/ Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens Mohamed Sohaimi, Norfitriah Bejo, Mohd Hair Omar, Abdul Rahman Ideris, Aini Mat Isa, Nurulfiza Fowl adenovirus (FAdV) is the causative agent of inclusion body hepatitis (IBH) in chickens with significant economic losses due to high mortality and poor production. It was objectives of the study to attenuate and determine the molecular characteristic of FAdV isolate (UPM1137) of Malaysia passages in primary chicken embryo liver (CEL) cells. The cytopathic effect (CPE) was recorded and the present of the virus was detected by polymerase chain reaction (PCR). Nucleotide and amino acid changes were determined and a phylogenetic tree was constructed. The pathogenicity and immunogenicity of the virus at passage 35 (CEL35) with virus titre of 106.7TCID50/mL was determined in day old specific pathogen free (SPF) chicks via oral or subcutaneous route of inoculation. The study demonstrated that the FAdV isolate was successfully propagated and attenuated in CEL cells up to 35th consecutive passages (CEL35) with delayed of CPE formation within 48 to 72 post inoculation (pi) from CEL20 onwards. The virus caused typical CPE with basophilic intranuclear inclusion bodies, refractile and clumping of cells. The virus is belong to serotype 8b with substitution of amino acid at position 44, 133 and 185 in L1 loop of hexon gene and in knob of fiber gene at position 348 and 360 at CEL35. It is non-pathogenic, but immunogenic in SPF chickens. It was concluded that the FAdV isolate was successfully attenuated in CEL cells with molecular changes in major capsid proteins which affect its infectivity in cell culture and SPF chickens. Public Library of Science 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81403/1/FOWL.pdf Mohamed Sohaimi, Norfitriah and Bejo, Mohd Hair and Omar, Abdul Rahman and Ideris, Aini and Mat Isa, Nurulfiza (2019) Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens. PLoS One, 14 (12). pp. 1-16. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225863 10.1371/journal.pone.0225863
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Fowl adenovirus (FAdV) is the causative agent of inclusion body hepatitis (IBH) in chickens with significant economic losses due to high mortality and poor production. It was objectives of the study to attenuate and determine the molecular characteristic of FAdV isolate (UPM1137) of Malaysia passages in primary chicken embryo liver (CEL) cells. The cytopathic effect (CPE) was recorded and the present of the virus was detected by polymerase chain reaction (PCR). Nucleotide and amino acid changes were determined and a phylogenetic tree was constructed. The pathogenicity and immunogenicity of the virus at passage 35 (CEL35) with virus titre of 106.7TCID50/mL was determined in day old specific pathogen free (SPF) chicks via oral or subcutaneous route of inoculation. The study demonstrated that the FAdV isolate was successfully propagated and attenuated in CEL cells up to 35th consecutive passages (CEL35) with delayed of CPE formation within 48 to 72 post inoculation (pi) from CEL20 onwards. The virus caused typical CPE with basophilic intranuclear inclusion bodies, refractile and clumping of cells. The virus is belong to serotype 8b with substitution of amino acid at position 44, 133 and 185 in L1 loop of hexon gene and in knob of fiber gene at position 348 and 360 at CEL35. It is non-pathogenic, but immunogenic in SPF chickens. It was concluded that the FAdV isolate was successfully attenuated in CEL cells with molecular changes in major capsid proteins which affect its infectivity in cell culture and SPF chickens.
format Article
author Mohamed Sohaimi, Norfitriah
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
Mat Isa, Nurulfiza
spellingShingle Mohamed Sohaimi, Norfitriah
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
Mat Isa, Nurulfiza
Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens
author_facet Mohamed Sohaimi, Norfitriah
Bejo, Mohd Hair
Omar, Abdul Rahman
Ideris, Aini
Mat Isa, Nurulfiza
author_sort Mohamed Sohaimi, Norfitriah
title Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens
title_short Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens
title_full Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens
title_fullStr Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens
title_full_unstemmed Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens
title_sort molecular characterization of fowl adenovirus isolate of malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens
publisher Public Library of Science
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/81403/1/FOWL.pdf
http://psasir.upm.edu.my/id/eprint/81403/
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225863
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score 13.211869

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