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Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats

Introduction: Natural products are obtaining much acceptance as ergogenic aid, not only among athletes but also among the general population including people with excess body fat. Under normal circumstances, an obese person will have the desire and ability to exercise reduced; mainly because they ar...

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Main Authors: Abdul Majid, Nordiana, Abdul Hamid, Azizah, Salleh, Syafiq Zikri, Saari, Nazamid, Abas, Faridah, Pak Dek, Mohd Sabri, Ramli, Nurul Shazini, Jaafar, Ahmad Haniff
Format: Article
Language:English
Published: John Wiley and Sons 2019
Online Access:http://psasir.upm.edu.my/id/eprint/81338/1/MORINDA.pdf
http://psasir.upm.edu.my/id/eprint/81338/
https://pubmed.ncbi.nlm.nih.gov/31381209/
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spelling my.upm.eprints.813382021-06-14T09:37:08Z http://psasir.upm.edu.my/id/eprint/81338/ Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats Abdul Majid, Nordiana Abdul Hamid, Azizah Salleh, Syafiq Zikri Saari, Nazamid Abas, Faridah Pak Dek, Mohd Sabri Ramli, Nurul Shazini Jaafar, Ahmad Haniff Introduction: Natural products are obtaining much acceptance as ergogenic aid, not only among athletes but also among the general population including people with excess body fat. Under normal circumstances, an obese person will have the desire and ability to exercise reduced; mainly because they are easily fatigued. Thus, they need to boost their energy production so that they can be more active and healthier. Objective: In this present work, Morinda citrifolia L. leaf extract (MLE) which is believed to possess ergogenic property, was evaluated on its effect on an obese animal model using 1 H-NMR based metabolomics. Material and methods: Rats were fed with high fat diet (HFD) for 12 weeks for obese development. Once this was achieved, all the rats underwent endurance exercise (forced swimming test) every 2 weeks for 8 weeks together with treatment. The time to exhaustion was recorded for each rat. Three different dosages of MLE: 50 mg/kg, 100 mg/kg and 200 mg/kg of body weight were used together with two positive controls: 5 mg/kg caffeine and 100 mg/kg green tea. Blood was collected before and after treatments for metabolomics study. Results: Findings showed that feeding the rats at a dose of 200 mg/kg body weight MLE significantly prolonged the exhaustive swimming time of the rats, and altered the metabolites present in their serum. Discriminating metabolites involved were the product of various metabolic pathways, including carbohydrate, lipids metabolism and energy metabolism. Treatment with 200 mg/kg body weight MLE resulted in significant improvement in the metabolic perturbations where the proximity of the obese exercised treated group to that of normal exercised group in the partial least squares discriminant analysis score plot was observed. Conclusion: The present work demonstrated ergogenic property of MLE based on the improved metabolic perturbation in exercised obese rats. John Wiley and Sons 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81338/1/MORINDA.pdf Abdul Majid, Nordiana and Abdul Hamid, Azizah and Salleh, Syafiq Zikri and Saari, Nazamid and Abas, Faridah and Pak Dek, Mohd Sabri and Ramli, Nurul Shazini and Jaafar, Ahmad Haniff (2019) Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats. Phytochemical Analysis, 31 (2). pp. 191-203. ISSN 0958-0344; ESSN: 1099-1565 https://pubmed.ncbi.nlm.nih.gov/31381209/ 10.1002/pca.2880
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: Natural products are obtaining much acceptance as ergogenic aid, not only among athletes but also among the general population including people with excess body fat. Under normal circumstances, an obese person will have the desire and ability to exercise reduced; mainly because they are easily fatigued. Thus, they need to boost their energy production so that they can be more active and healthier. Objective: In this present work, Morinda citrifolia L. leaf extract (MLE) which is believed to possess ergogenic property, was evaluated on its effect on an obese animal model using 1 H-NMR based metabolomics. Material and methods: Rats were fed with high fat diet (HFD) for 12 weeks for obese development. Once this was achieved, all the rats underwent endurance exercise (forced swimming test) every 2 weeks for 8 weeks together with treatment. The time to exhaustion was recorded for each rat. Three different dosages of MLE: 50 mg/kg, 100 mg/kg and 200 mg/kg of body weight were used together with two positive controls: 5 mg/kg caffeine and 100 mg/kg green tea. Blood was collected before and after treatments for metabolomics study. Results: Findings showed that feeding the rats at a dose of 200 mg/kg body weight MLE significantly prolonged the exhaustive swimming time of the rats, and altered the metabolites present in their serum. Discriminating metabolites involved were the product of various metabolic pathways, including carbohydrate, lipids metabolism and energy metabolism. Treatment with 200 mg/kg body weight MLE resulted in significant improvement in the metabolic perturbations where the proximity of the obese exercised treated group to that of normal exercised group in the partial least squares discriminant analysis score plot was observed. Conclusion: The present work demonstrated ergogenic property of MLE based on the improved metabolic perturbation in exercised obese rats.
format Article
author Abdul Majid, Nordiana
Abdul Hamid, Azizah
Salleh, Syafiq Zikri
Saari, Nazamid
Abas, Faridah
Pak Dek, Mohd Sabri
Ramli, Nurul Shazini
Jaafar, Ahmad Haniff
spellingShingle Abdul Majid, Nordiana
Abdul Hamid, Azizah
Salleh, Syafiq Zikri
Saari, Nazamid
Abas, Faridah
Pak Dek, Mohd Sabri
Ramli, Nurul Shazini
Jaafar, Ahmad Haniff
Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats
author_facet Abdul Majid, Nordiana
Abdul Hamid, Azizah
Salleh, Syafiq Zikri
Saari, Nazamid
Abas, Faridah
Pak Dek, Mohd Sabri
Ramli, Nurul Shazini
Jaafar, Ahmad Haniff
author_sort Abdul Majid, Nordiana
title Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats
title_short Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats
title_full Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats
title_fullStr Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats
title_full_unstemmed Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats
title_sort metabolomics approach to investigate the ergogenic effect of morinda citrifolia l. leaf extract on obese sprague dawley rats
publisher John Wiley and Sons
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/81338/1/MORINDA.pdf
http://psasir.upm.edu.my/id/eprint/81338/
https://pubmed.ncbi.nlm.nih.gov/31381209/
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score 13.211869

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