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Cholinesterase from the liver of Diodon hystrix for detection of metal ions

The discharge of industrial effluents into nearby water bodies affects the inhabitants including living organisms. The presence of foreign materials such as heavy metals can be a threat to the ecosystem as they are enormously carcinogenic even though in minute concentration. Hence, an economical and...

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Main Authors: Nordin, Noreen, Cletus, Ronaldo Ron, Sabullah, Mohd Khalizan, Muhammad Khalidi, Siti Aishah, Abdulla, Rahmath, Ahmad, Siti Aqlima
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2020
Online Access:http://psasir.upm.edu.my/id/eprint/81126/1/METAL.pdf
http://psasir.upm.edu.my/id/eprint/81126/
http://www.pertanika.upm.edu.my/pjst/browse/special-issue?article=JST(S)-0562-2020
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spelling my.upm.eprints.811262021-08-17T22:33:16Z http://psasir.upm.edu.my/id/eprint/81126/ Cholinesterase from the liver of Diodon hystrix for detection of metal ions Nordin, Noreen Cletus, Ronaldo Ron Sabullah, Mohd Khalizan Muhammad Khalidi, Siti Aishah Abdulla, Rahmath Ahmad, Siti Aqlima The discharge of industrial effluents into nearby water bodies affects the inhabitants including living organisms. The presence of foreign materials such as heavy metals can be a threat to the ecosystem as they are enormously carcinogenic even though in minute concentration. Hence, an economical and time-efficient preliminary screening test is crucial to be developed for the detection of heavy metals, prior to employment of high technology instruments. In this study, cholinesterase (ChE) from Sabah porcupine fish, Diodon hystrix was purified to test for its potential as an alternative biosensor in detecting metal ions. Few enzymatic parameters including specificity of substrate, temperature and pH were applied to determine its optimal enzymatic activity. ChE enzyme was found to be more sensitive towards the presence of substrate, butyrylthiocholine iodide (BTC), in contrast to acetylthiocholine iodide (ATC) and propionylthiocholine iodide (PTC) with the effective coefficient at 7193, 3680.15 and 2965.26 Vmax/Km, respectively. Moreover, the extracted ChE enzyme showed the optimum activity at pH 9 of 0.1 M Tris-HCl and at 25°C to 30°C range of temperature. When subjected to heavy metals, ChE enzyme was significantly inhibited as the enzyme activity was reduced in the sequence of Hg > Ag > Cr > Cu > Cd > Pb ≥ Zn > As. As a conclusion, the partially purified ChE enzyme proved its sensitivity towards metal ion exposure and can be used as an alternative method in screening the level of contamination in the environment. Universiti Putra Malaysia Press 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81126/1/METAL.pdf Nordin, Noreen and Cletus, Ronaldo Ron and Sabullah, Mohd Khalizan and Muhammad Khalidi, Siti Aishah and Abdulla, Rahmath and Ahmad, Siti Aqlima (2020) Cholinesterase from the liver of Diodon hystrix for detection of metal ions. Pertanika Journal of Science and Technology, 28 (spec. 2). pp. 107-119. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/special-issue?article=JST(S)-0562-2020 10.47836/pjst.28.S2.09
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The discharge of industrial effluents into nearby water bodies affects the inhabitants including living organisms. The presence of foreign materials such as heavy metals can be a threat to the ecosystem as they are enormously carcinogenic even though in minute concentration. Hence, an economical and time-efficient preliminary screening test is crucial to be developed for the detection of heavy metals, prior to employment of high technology instruments. In this study, cholinesterase (ChE) from Sabah porcupine fish, Diodon hystrix was purified to test for its potential as an alternative biosensor in detecting metal ions. Few enzymatic parameters including specificity of substrate, temperature and pH were applied to determine its optimal enzymatic activity. ChE enzyme was found to be more sensitive towards the presence of substrate, butyrylthiocholine iodide (BTC), in contrast to acetylthiocholine iodide (ATC) and propionylthiocholine iodide (PTC) with the effective coefficient at 7193, 3680.15 and 2965.26 Vmax/Km, respectively. Moreover, the extracted ChE enzyme showed the optimum activity at pH 9 of 0.1 M Tris-HCl and at 25°C to 30°C range of temperature. When subjected to heavy metals, ChE enzyme was significantly inhibited as the enzyme activity was reduced in the sequence of Hg > Ag > Cr > Cu > Cd > Pb ≥ Zn > As. As a conclusion, the partially purified ChE enzyme proved its sensitivity towards metal ion exposure and can be used as an alternative method in screening the level of contamination in the environment.
format Article
author Nordin, Noreen
Cletus, Ronaldo Ron
Sabullah, Mohd Khalizan
Muhammad Khalidi, Siti Aishah
Abdulla, Rahmath
Ahmad, Siti Aqlima
spellingShingle Nordin, Noreen
Cletus, Ronaldo Ron
Sabullah, Mohd Khalizan
Muhammad Khalidi, Siti Aishah
Abdulla, Rahmath
Ahmad, Siti Aqlima
Cholinesterase from the liver of Diodon hystrix for detection of metal ions
author_facet Nordin, Noreen
Cletus, Ronaldo Ron
Sabullah, Mohd Khalizan
Muhammad Khalidi, Siti Aishah
Abdulla, Rahmath
Ahmad, Siti Aqlima
author_sort Nordin, Noreen
title Cholinesterase from the liver of Diodon hystrix for detection of metal ions
title_short Cholinesterase from the liver of Diodon hystrix for detection of metal ions
title_full Cholinesterase from the liver of Diodon hystrix for detection of metal ions
title_fullStr Cholinesterase from the liver of Diodon hystrix for detection of metal ions
title_full_unstemmed Cholinesterase from the liver of Diodon hystrix for detection of metal ions
title_sort cholinesterase from the liver of diodon hystrix for detection of metal ions
publisher Universiti Putra Malaysia Press
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/81126/1/METAL.pdf
http://psasir.upm.edu.my/id/eprint/81126/
http://www.pertanika.upm.edu.my/pjst/browse/special-issue?article=JST(S)-0562-2020
_version_ 1709668988192030720
score 13.211869

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