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Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring

Cholinesterase-based biosensor well known as a sensitive method to detect the existence of harmful dissolved compounds in any type of water source, especially the river. This alternative biosensor can be used to determine the level of pollution of the water in a short period of time as well as to e...

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Main Authors: Muhammad Khalidi, Siti Aishah, Sabullah, Mohd Khalizan, Abdul Sani, Suraya, Abd Shukor, Mohd Yunus, Basirun, Ain Aqilah, Abd Gafar, Aa'ishah, Mohd Jaafar, Izazy Nur, Nordin, Noreen
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2020
Online Access:http://psasir.upm.edu.my/id/eprint/81111/1/DNA.pdf
http://psasir.upm.edu.my/id/eprint/81111/
http://www.pertanika.upm.edu.my/pjst/browse/special-issue?article=JST(S)-0560-2020
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spelling my.upm.eprints.811112021-08-17T22:40:01Z http://psasir.upm.edu.my/id/eprint/81111/ Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring Muhammad Khalidi, Siti Aishah Sabullah, Mohd Khalizan Abdul Sani, Suraya Abd Shukor, Mohd Yunus Basirun, Ain Aqilah Abd Gafar, Aa'ishah Mohd Jaafar, Izazy Nur Nordin, Noreen Cholinesterase-based biosensor well known as a sensitive method to detect the existence of harmful dissolved compounds in any type of water source, especially the river. This alternative biosensor can be used to determine the level of pollution of the water in a short period of time as well as to evaluate the low cost and simple service. The aim of this study was to exceed the effectiveness of acetylcholinesterase source extracted from the brain tissue of Asian swamp eel; Monopterus albus as a potential environmental biosensor. Purified acetylcholinesterase exposed to a different type of metal ions and mercury showed the highest percentage of inhibition at 62.9% followed by chromium at 59.22% while silver, arsenic, cadmium, cobalt, copper, nickel, zinc and lead at not more than 50% (approximately 37-50%). Metal ions such as mercury, zinc, chromium and copper showed exponential decay type inhibition curves with calculated half maximal inhibitory concentration; IC50 in the ascending sensitivity order 0.005, 0.595, 0.687 and 1.329 mgL-1, respectively. Field trial works exhibited that the acetylcholinesterase was applicable in sensing heavy metals pollution from the river which closed to the industrial and agricultural sites at near real-time and verified using ICP-OES. This study proves the potential use of acetylcholinesterase sourced from M. albus as a biomonitoring tool to assess the contamination level of the river. Universiti Putra Malaysia Press 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81111/1/DNA.pdf Muhammad Khalidi, Siti Aishah and Sabullah, Mohd Khalizan and Abdul Sani, Suraya and Abd Shukor, Mohd Yunus and Basirun, Ain Aqilah and Abd Gafar, Aa'ishah and Mohd Jaafar, Izazy Nur and Nordin, Noreen (2020) Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring. Pertanika Journal of Science and Technology, 28 (spec. 2). pp. 83-94. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/special-issue?article=JST(S)-0560-2020 10.47836/pjst.28.S2.07
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Cholinesterase-based biosensor well known as a sensitive method to detect the existence of harmful dissolved compounds in any type of water source, especially the river. This alternative biosensor can be used to determine the level of pollution of the water in a short period of time as well as to evaluate the low cost and simple service. The aim of this study was to exceed the effectiveness of acetylcholinesterase source extracted from the brain tissue of Asian swamp eel; Monopterus albus as a potential environmental biosensor. Purified acetylcholinesterase exposed to a different type of metal ions and mercury showed the highest percentage of inhibition at 62.9% followed by chromium at 59.22% while silver, arsenic, cadmium, cobalt, copper, nickel, zinc and lead at not more than 50% (approximately 37-50%). Metal ions such as mercury, zinc, chromium and copper showed exponential decay type inhibition curves with calculated half maximal inhibitory concentration; IC50 in the ascending sensitivity order 0.005, 0.595, 0.687 and 1.329 mgL-1, respectively. Field trial works exhibited that the acetylcholinesterase was applicable in sensing heavy metals pollution from the river which closed to the industrial and agricultural sites at near real-time and verified using ICP-OES. This study proves the potential use of acetylcholinesterase sourced from M. albus as a biomonitoring tool to assess the contamination level of the river.
format Article
author Muhammad Khalidi, Siti Aishah
Sabullah, Mohd Khalizan
Abdul Sani, Suraya
Abd Shukor, Mohd Yunus
Basirun, Ain Aqilah
Abd Gafar, Aa'ishah
Mohd Jaafar, Izazy Nur
Nordin, Noreen
spellingShingle Muhammad Khalidi, Siti Aishah
Sabullah, Mohd Khalizan
Abdul Sani, Suraya
Abd Shukor, Mohd Yunus
Basirun, Ain Aqilah
Abd Gafar, Aa'ishah
Mohd Jaafar, Izazy Nur
Nordin, Noreen
Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring
author_facet Muhammad Khalidi, Siti Aishah
Sabullah, Mohd Khalizan
Abdul Sani, Suraya
Abd Shukor, Mohd Yunus
Basirun, Ain Aqilah
Abd Gafar, Aa'ishah
Mohd Jaafar, Izazy Nur
Nordin, Noreen
author_sort Muhammad Khalidi, Siti Aishah
title Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring
title_short Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring
title_full Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring
title_fullStr Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring
title_full_unstemmed Acetylcholine receptor-based biosensor derived from Asian swamp Eel, monopterus albus for heavy metals biomonitoring
title_sort acetylcholine receptor-based biosensor derived from asian swamp eel, monopterus albus for heavy metals biomonitoring
publisher Universiti Putra Malaysia Press
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/81111/1/DNA.pdf
http://psasir.upm.edu.my/id/eprint/81111/
http://www.pertanika.upm.edu.my/pjst/browse/special-issue?article=JST(S)-0560-2020
_version_ 1709668987903672320
score 13.211869

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