Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration
Blindness and vision loss contribute to irreversible retinal degeneration, and cellular therapy for retinal cell replacement has the potential to treat individuals who have lost light sensitive photoreceptors in the retina. Retinal cells are well characterized in function, and are a subject of inter...
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Elsevier
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/81092/1/DENTAL.pdf http://psasir.upm.edu.my/id/eprint/81092/ https://www.sciencedirect.com/science/article/pii/S1011134419304944 |
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my.upm.eprints.810922020-11-06T19:10:18Z http://psasir.upm.edu.my/id/eprint/81092/ Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration Subbiah, Suresh Kumar Teh, Seoh Wei Koh, Avin Ee Hwan Alsaeedi, Hiba Amer Lam, Chen Shen Abd Rashid, Munirah Harun, Mohd Hairul Nizam Mohd Saleh, Muhamad Fakhri Luu, Chi D. Ng, Min Hwei Mohd Isa, Hazlita Leow, Sue Ngein Then, Kong Yong Bastion, Mae-Lynn Catherine Mok, Pooi Ling Muthuvenkatachalam, Bala Sundaram Samrot, Anthony V. Swamy, K. B. Nandakumar, Jaikumar Blindness and vision loss contribute to irreversible retinal degeneration, and cellular therapy for retinal cell replacement has the potential to treat individuals who have lost light sensitive photoreceptors in the retina. Retinal cells are well characterized in function, and are a subject of interest in cellular replacement therapy of photoreceptors and the retinal pigment epithelium. However, retinal cell transplantation is limited by various factors, including the choice of potential stem cell source that can show variability in plasticity as well as host tissue integration. Dental pulp is one such source that contains an abundance of stem cells. In this study we used dental pulp-derived mesenchymal stem cells (DPSCs) to mitigate sodium iodate (NaIO3) insult in a rat model of retinal degeneration. Sprague-Dawley rats were first given an intravitreal injection of 3 × 105 DPSCs as well as a single systemic administration of NaIO3 (40 mg/kg). Electroretinography (ERG) was performed for the next two months and was followed-up by histological analysis. The ERG recordings showed protection of DPSC-treated retinas within 4 weeks, which was statistically significant (* P ≤ .05) compared to the control. Retinal thickness of the control was also found to be thinner (*** P ≤ .001). The DPSCs were found integrated in the photoreceptor layer through immunohistochemical staining. Our findings showed that DPSCs have the potential to moderate retinal degeneration. In conclusion, DPSCs are a potential source of stem cells in the field of eye stem cell therapy due to its protective effects against retinal degeneration. Elsevier 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/81092/1/DENTAL.pdf Subbiah, Suresh Kumar and Teh, Seoh Wei and Koh, Avin Ee Hwan and Alsaeedi, Hiba Amer and Lam, Chen Shen and Abd Rashid, Munirah and Harun, Mohd Hairul Nizam and Mohd Saleh, Muhamad Fakhri and Luu, Chi D. and Ng, Min Hwei and Mohd Isa, Hazlita and Leow, Sue Ngein and Then, Kong Yong and Bastion, Mae-Lynn Catherine and Mok, Pooi Ling and Muthuvenkatachalam, Bala Sundaram and Samrot, Anthony V. and Swamy, K. B. and Nandakumar, Jaikumar (2019) Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration. Journal of Photochemistry and Photobiology, B-Biology, 198. pp. 1-8. ISSN 1011-1344 https://www.sciencedirect.com/science/article/pii/S1011134419304944 10.1016/j.jphotobiol.2019.111561 |
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Blindness and vision loss contribute to irreversible retinal degeneration, and cellular therapy for retinal cell replacement has the potential to treat individuals who have lost light sensitive photoreceptors in the retina. Retinal cells are well characterized in function, and are a subject of interest in cellular replacement therapy of photoreceptors and the retinal pigment epithelium. However, retinal cell transplantation is limited by various factors, including the choice of potential stem cell source that can show variability in plasticity as well as host tissue integration. Dental pulp is one such source that contains an abundance of stem cells. In this study we used dental pulp-derived mesenchymal stem cells (DPSCs) to mitigate sodium iodate (NaIO3) insult in a rat model of retinal degeneration. Sprague-Dawley rats were first given an intravitreal injection of 3 × 105 DPSCs as well as a single systemic administration of NaIO3 (40 mg/kg). Electroretinography (ERG) was performed for the next two months and was followed-up by histological analysis. The ERG recordings showed protection of DPSC-treated retinas within 4 weeks, which was statistically significant (* P ≤ .05) compared to the control. Retinal thickness of the control was also found to be thinner (*** P ≤ .001). The DPSCs were found integrated in the photoreceptor layer through immunohistochemical staining. Our findings showed that DPSCs have the potential to moderate retinal degeneration. In conclusion, DPSCs are a potential source of stem cells in the field of eye stem cell therapy due to its protective effects against retinal degeneration. |
| format |
Article |
| author |
Subbiah, Suresh Kumar Teh, Seoh Wei Koh, Avin Ee Hwan Alsaeedi, Hiba Amer Lam, Chen Shen Abd Rashid, Munirah Harun, Mohd Hairul Nizam Mohd Saleh, Muhamad Fakhri Luu, Chi D. Ng, Min Hwei Mohd Isa, Hazlita Leow, Sue Ngein Then, Kong Yong Bastion, Mae-Lynn Catherine Mok, Pooi Ling Muthuvenkatachalam, Bala Sundaram Samrot, Anthony V. Swamy, K. B. Nandakumar, Jaikumar |
| spellingShingle |
Subbiah, Suresh Kumar Teh, Seoh Wei Koh, Avin Ee Hwan Alsaeedi, Hiba Amer Lam, Chen Shen Abd Rashid, Munirah Harun, Mohd Hairul Nizam Mohd Saleh, Muhamad Fakhri Luu, Chi D. Ng, Min Hwei Mohd Isa, Hazlita Leow, Sue Ngein Then, Kong Yong Bastion, Mae-Lynn Catherine Mok, Pooi Ling Muthuvenkatachalam, Bala Sundaram Samrot, Anthony V. Swamy, K. B. Nandakumar, Jaikumar Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration |
| author_facet |
Subbiah, Suresh Kumar Teh, Seoh Wei Koh, Avin Ee Hwan Alsaeedi, Hiba Amer Lam, Chen Shen Abd Rashid, Munirah Harun, Mohd Hairul Nizam Mohd Saleh, Muhamad Fakhri Luu, Chi D. Ng, Min Hwei Mohd Isa, Hazlita Leow, Sue Ngein Then, Kong Yong Bastion, Mae-Lynn Catherine Mok, Pooi Ling Muthuvenkatachalam, Bala Sundaram Samrot, Anthony V. Swamy, K. B. Nandakumar, Jaikumar |
| author_sort |
Subbiah, Suresh Kumar |
| title |
Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration |
| title_short |
Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration |
| title_full |
Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration |
| title_fullStr |
Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration |
| title_full_unstemmed |
Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration |
| title_sort |
dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration |
| publisher |
Elsevier |
| publishDate |
2019 |
| url |
http://psasir.upm.edu.my/id/eprint/81092/1/DENTAL.pdf http://psasir.upm.edu.my/id/eprint/81092/ https://www.sciencedirect.com/science/article/pii/S1011134419304944 |
| _version_ |
1683232245302689792 |
| score |
13.211869 |