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Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium

Aim : Cholinesterase (ChE) inhibition and histopathological features of brain tissues of Clarias gariepinus were investigated with a purpose to develop biomarkers for monitoring heavy metals. Methodology : The effects of cadmium toxicity on were assessed by ChE inhibition and characterisation of ChE...

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Main Authors: Fadzil, Nadia Izati, Ahmad, Siti Aqlima, Yasid, Nur Adeela, Sabullah, Mohd Khalizan, Mohd Daud, Hassan, Khalid, Ariff, Padrilah, Siti Nadzirah, Shukor, Mohd Yunus
Format: Article
Language:English
Published: Triveni 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80897/1/CADMIUM.pdf
http://psasir.upm.edu.my/id/eprint/80897/
http://www.jeb.co.in/index.php?page=issue_toc&issue=201903_mar19
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spelling my.upm.eprints.808972020-10-15T22:59:23Z http://psasir.upm.edu.my/id/eprint/80897/ Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium Fadzil, Nadia Izati Ahmad, Siti Aqlima Yasid, Nur Adeela Sabullah, Mohd Khalizan Mohd Daud, Hassan Khalid, Ariff Padrilah, Siti Nadzirah Shukor, Mohd Yunus Aim : Cholinesterase (ChE) inhibition and histopathological features of brain tissues of Clarias gariepinus were investigated with a purpose to develop biomarkers for monitoring heavy metals. Methodology : The effects of cadmium toxicity on were assessed by ChE inhibition and characterisation of ChE by affinity chromatography and histological variations in brain tissues were studied by H&E staining under light microscope. Results : The purified enzyme gave 10.58 fold purification with the recovery of 12% with specific activity of 159.729 U-1 mg . The Michaelis-Menten constant (K ) m and V value obtained was 1.985 mM max -1 -1 and 0.4479 µmol min mg , respectively. The enzyme showed the ability to hydrolyse acetylthiocholine iodide (ATC) at a faster rate compared to other two synthetic substrates, propionylcholine iodide (PTC) and butyrylthiocholine iodide (BTC). ChE showed highest activity at 35°C in Tris-HCl pH 7.5. The metal inhibition study also found the maximal inhibitory concentration (IC ) 50 -1 value for cadmium to be 0.6517 mg l . Histological study revealed decrease in the number of cells, degeneration of Purkinje cells, increased aggregation area and cells necrosis. Interpretation : Inhibition of cholinesterase activity and degeneration of Purkinje cells observed in the present study can be used as a tool for further developing a biomarker for detecting heavy metals in the aquatic ecosystems. C. gariepinus Triveni 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80897/1/CADMIUM.pdf Fadzil, Nadia Izati and Ahmad, Siti Aqlima and Yasid, Nur Adeela and Sabullah, Mohd Khalizan and Mohd Daud, Hassan and Khalid, Ariff and Padrilah, Siti Nadzirah and Shukor, Mohd Yunus (2019) Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium. Journal of Environmental Biology, 40 (22). pp. 133-142. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=issue_toc&issue=201903_mar19 10.22438/jeb/40/2/MRN-815
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Aim : Cholinesterase (ChE) inhibition and histopathological features of brain tissues of Clarias gariepinus were investigated with a purpose to develop biomarkers for monitoring heavy metals. Methodology : The effects of cadmium toxicity on were assessed by ChE inhibition and characterisation of ChE by affinity chromatography and histological variations in brain tissues were studied by H&E staining under light microscope. Results : The purified enzyme gave 10.58 fold purification with the recovery of 12% with specific activity of 159.729 U-1 mg . The Michaelis-Menten constant (K ) m and V value obtained was 1.985 mM max -1 -1 and 0.4479 µmol min mg , respectively. The enzyme showed the ability to hydrolyse acetylthiocholine iodide (ATC) at a faster rate compared to other two synthetic substrates, propionylcholine iodide (PTC) and butyrylthiocholine iodide (BTC). ChE showed highest activity at 35°C in Tris-HCl pH 7.5. The metal inhibition study also found the maximal inhibitory concentration (IC ) 50 -1 value for cadmium to be 0.6517 mg l . Histological study revealed decrease in the number of cells, degeneration of Purkinje cells, increased aggregation area and cells necrosis. Interpretation : Inhibition of cholinesterase activity and degeneration of Purkinje cells observed in the present study can be used as a tool for further developing a biomarker for detecting heavy metals in the aquatic ecosystems. C. gariepinus
format Article
author Fadzil, Nadia Izati
Ahmad, Siti Aqlima
Yasid, Nur Adeela
Sabullah, Mohd Khalizan
Mohd Daud, Hassan
Khalid, Ariff
Padrilah, Siti Nadzirah
Shukor, Mohd Yunus
spellingShingle Fadzil, Nadia Izati
Ahmad, Siti Aqlima
Yasid, Nur Adeela
Sabullah, Mohd Khalizan
Mohd Daud, Hassan
Khalid, Ariff
Padrilah, Siti Nadzirah
Shukor, Mohd Yunus
Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium
author_facet Fadzil, Nadia Izati
Ahmad, Siti Aqlima
Yasid, Nur Adeela
Sabullah, Mohd Khalizan
Mohd Daud, Hassan
Khalid, Ariff
Padrilah, Siti Nadzirah
Shukor, Mohd Yunus
author_sort Fadzil, Nadia Izati
title Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium
title_short Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium
title_full Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium
title_fullStr Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium
title_full_unstemmed Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium
title_sort characterisation of cholinesterase and histopathological features of brain of clarias gariepinus following exposure to cadmium
publisher Triveni
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80897/1/CADMIUM.pdf
http://psasir.upm.edu.my/id/eprint/80897/
http://www.jeb.co.in/index.php?page=issue_toc&issue=201903_mar19
_version_ 1681490828472614912
score 13.211869

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